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7 protocols using cuprous chloride

1

Synthesis and Antimicrobial Evaluation of POcQASs

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Tetrakis(triphenylphosphine)palladium(0) (Pd(PEt3)4) (CAS: 14221-01-3), copper iodide (CuI) (CAS: 7681-65-4), 1,3,5-tribromobenzene (C6H3Br3) (CAS: 626-39-1), triphenylphosphine (PPh3) (CAS: 603-35-0), (trimethylsilyl)acetylene (C5H10Si) (CAS: 1066-54-2), dichloromethane (DCM) (CAS: 75-09-2), anhydrous magnesium sulfate (MgSO4) (CAS: 139939-75-6), sodium bicarbonate (NaHCO3) (CAS: 144-55-8), petroleum ether (PE) (CAS: 8032-32-4), ethyl acetate (EA) (CAS: 141-78-6), tetrabutylammonium fluoride (TBAF), solution in THF (1M) (CAS: 429-41-4), N-hexane (C6H14) (CAS: 110-54-3), dichlorobis(triethylphosphine)-palladium(II) (Pd(PEt3)2Cl2) (CAS: 28425-04-9), cuprous chloride (CuCl) (CAS: 7758-89-6), triethylamine (NEt3) (CAS: 121-44-8), and N,N-dimethyloctylamine (C10H23N) (CAS: 7378-99-6) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other raw materials used for the preparation of monomers and S-POcQASs were the same as those used for our previous preparation of L-POcQASs. E. coli (ATCC25922) and S. aureus (ATCC25923) were obtained from the first affiliated hospital of Harbin Medical University (Harbin, China).
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2

Synthesis of Nanocomposite Materials

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Anhydrous
ethanol, zinc
acetate dihydrate, sodium hydroxide, cuprous chloride, and iron chloride
were purchased from Sigma-Aldrich. Polyethylene glycol (PEG) 400,
ethylene glycol, MB, neutral red, methyl red, and basic red were obtained
from Saen Chemical Technology (Shanghai) Co., Ltd. Polyacrylamide
(PAM), azobisisobutyronitrile (AIBN), epichlorohydrin (ECH), vinyltriethoxysilane
(VTEO), and 3-aminopropyl triethoxysilane (APTES) were purchased from
Shanghai Aladdin Biotechnology Co., Ltd. Each reagent and solvent
adopted in this experiment was analytically pure, which was utilized
as received.
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3

Fabrication and Characterization of Fused-Silica Microfluidic Devices

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Fused-silica capillaries (50-μm id, 360-μm od and 20-μm polyimide coating) were purchased from Biotaq Inc. (Silver Springs, MD, U.S.) and Polymicro Technologies (Phoenix, AZ, U.S.). UV-grade fused-silica wafers (25.4 × 9.0 × 1.0 mm) were purchased from Laser Optex Inc. (Beijin, China). Silica micro-particles (Nucleosil, 7-μm diameter and 91.0-m2 g−1 surface area, and YMC Gel, 10-μm diameter and 288-m2 g−1 surface area) were obtained from Macherey-Nagel (Duren, Germany) and YMC Co. (Kioto, Japan) respectively. Piperazine-N, N′-bis(3-propanesulfonic acid) (PIPPS) was purchased from GFS Chemicals (Columbus, OH, U.S.). Nacryloyl-aminoethanol, N-acryloyl-aminopropanol, 2-hydroxyethyl methacrylate, α-bromoisobutyryl bromide, tris[2-(dimethylamino)ethyl]amine (Me6TREN), bipyridine (bpy), allyl alcohol, platinum(0)-1,3-divinyl-1,1,3,3-tetramethyldisiloxane (Karstedt’s catalyst, ~2 % Pt in xylene), hexachloroplatinic acid, cuprous chloride, 2,5-di-tert-butylhydroquinone, potassium cyanide, EDTA, 3-(N-morpholino)propanesulfonic acid (MOPS), methylcellulose, Ampholytes pH 3-10 and pH 6–8 (40% aqueous solutions) and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, U.S.). Analytical grade solvents were obtained from various vendors; they were freshly distilled from sodium shavings before use.
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4

Synthesis of Methylene-bisanthranilic Acid

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Methylene-bisanthranilic acid, N-methylpyrrolidone (NMP), and cuprous chloride were purchased from Sigma-Aldrich (Schnelldorf, Germany) and used without additional purification
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5

Synthesis of Copper Sulfide Nanoparticles

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Sodium hypophosphite monohydrate (NaH2PO2•H2O, 99 %) and elemental sulfur (S, 99.98 %) were purchased from Uni-Chem. Cuprous chloride (CuCl, 99.99 %), trioctylphosphine oxide (TOPO, 90 %), oleylamine (OLA, 70 %), trioctylphosphine (TOP, 97 %), Nafion ® 117 solution (5 wt.%), acetonitrile (99 %), and ethyl acetate (99 %) were purchased from Sigma Aldrich.
All chemicals and solvents were used as received unless stated otherwise.
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6

Synthesis and Characterization of Surface-Functionalized Nanoparticles

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2-Acrylamido-2-methylpropanesulfonic acid (AMPS, >99%), triethylamine (TEA, >99%), methylbenzene and ethanol were purchased from Kelong Co., Ltd. (Chengdu, China). 3-Aminopropyltriethoxysilane (APTES, >96%) and 2-bromoisobutyryl bromide (BIBB, >98%) were purchased from Aladdin Industrial Co. (Shanghai, China). 1,1,4,7,10,10-Hexamethyl-triethylenetetramine (HMDETA, >97%) was purchased from best-reagent Chemical Reagent Co. (Chengdu, China). Cuprous chloride, cupric chloride and Pb(ii) ion standard solution (1000 mg L−1) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All chemical reagents and solvents were of analytical grade and used without further purification. Deionized water used in the following experiments was purified using an ultrapure reverse osmosis system (Chengdu Ultrapure Technology Co. China).
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7

Phytochemical Isolation and Characterization

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Berberine Cl, isocorydine HCl, cupric sulfate pentahydrate (CuSO4 ∙ 5H2O), cuprous chloride (CuCl), DMSO, ferric chloride hexahydrate (FeCl3 ∙ 6H2O), ferrous sulfate heptahydrate (FeSO4 ∙ 7H2O), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt (ferrozine), hydroxylamine hydrochloride (hydroxylamine), hematoxylin, hydrochloric acid (HCl), BCS, sodium chloride (NaCl), sodium acetate, acetic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), HEPES sodium salt and trientine were purchased from Sigma-Aldrich (Czech Republic), while deferoxamine was from Novartis (Switzerland), boldine was from Carl Roth (Germany), glaucine was from Cayman Chemical (Ann Arbor, MI, USA) and galanthamine hydrobromide was from Changsha Organic Herb (Changsha, China). Chlidanthine and galanthamine were isolated from Chlidanthus fragrans herb [30 (link)], haemanthamine, lycorine and galanthine from Zephyranthes robusta (Herb. ex Sweet) [31 (link)], bulbocapnine, corydine, sinoacutine, canadine, corydaline, scoulerine, tetrahydropalmatine, allocryptopine and corycavamine from Corydalis cava (L.) [32 (link)], californidine iodide, eschscholtzine and protopine from Eschscholia californica [33 (link)], platycerine from Argemona platyceras [34 (link)] and sinactine, stylopine, cryptopine, fumaricine and parfumine from Fumaria officinalis L. [35 (link)].
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