Cbd d3

Manufactured by Cerilliant

Sourced in United States

CBD-d3 is a deuterated cannabidiol reference standard manufactured by Cerilliant. It is intended for use in analytical and research applications.

Automatically generated - may contain errors

Lab products found in correlation

Thc d3, by Cerilliant (13 mentions) Cbn d3, by Cerilliant (10 mentions) Methanol, by Thermo Fisher Scientific (4 mentions) Lc1100, by Agilent Technologies (4 mentions) Eclipse xdb c18 column, by Agilent Technologies (3 mentions) Thc d3, by Agilent Technologies (3 mentions) Formic acid, by Thermo Fisher Scientific (3 mentions) 11 oh thc, by Cerilliant (3 mentions) 11 oh thc d3, by Cerilliant (3 mentions) Thc cooh d9, by Cerilliant (2 mentions) Cbd d3, by Agilent Technologies (2 mentions) Msd6140 single quadrodpole, by Agilent Technologies (2 mentions) Thca a, by Cerilliant (2 mentions) Ammonium formate, by Thermo Fisher Scientific (2 mentions) Lc ms grade water, by Avantor (2 mentions) Acetonitrile, by Avantor (2 mentions) 2 propanol, by Thermo Fisher Scientific (2 mentions) Thccooh, by Cerilliant (2 mentions) δ9 thc, by Cerilliant (2 mentions) Thc oh, by Cerilliant (1 mentions)

18 protocols using cbd d3

Quantifying Cannabidiol in Canine and Feline Serum

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CBD was extracted from canine and feline serum using a combination of protein precipitation and liquid–liquid extraction using n-hexane as previously described [19 (link)], with minor modifications for microflow ultra-high pressure liquid chromatography (UHPLC). Briefly, 0.05 mL of canine and feline serum was subjected to protein precipitation in the presence of ice-cold acetonitrile (200 μL; 80% final concentration in distilled water), spiked with deuterated CBD as the internal standard (0.06 mg/mL, CBD-d3 Cerilliant, Round Rock, TX, USA). 0.2 mL of water was added to each sample prior to the addition of 1 mL of hexane to enhance liquid–liquid phase separation. Hexane extract was removed and dried under laboratory nitrogen. Prior to liquid chromatography–mass spectrometry (LC–MS) analysis, samples were resuspended in 0.06 mL of 100% acetonitrile. A standard curve using the CBD analytical standard was prepared in canine and feline serum non-exposed to CBD and extracted as above. Cannabidiol concentration in serum was quantified using a chromatographically coupled triple-quadrupole mass spectrometer (UHPLC–QQQ-MS) using similar methods as previously described [20 (link)].

Deabold K.A., Schwark W.S., Wolf L, & Wakshlag J.J. (2019). Single-Dose Pharmacokinetics and Preliminary Safety Assessment with Use of CBD-Rich Hemp Nutraceutical in Healthy Dogs and Cats. Animals : an Open Access Journal from MDPI, 9(10), 832.

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Quantification of Cannabinoids in Serum

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Reference standards for THC-d3, THC-OH, THC-OH-d3, THC-COOH-d9, CBD, CBD-d3, CBDA, CBN-d3, CBNA, CBG, CBGA, CBC, CBCA, CBL, THCV, THCVA, CBDV and CBDVA were purchased from Cerilliant (Round Rock, TX, USA), CBG-d9 and CBC-d9 from Cayman Chemical Co. (Ann Arbor, MI, USA) via LGC Standards (Wesel, Germany), CBN, THC and THC-COOH from LGC Standards and THCAA from THC Pharm (Frankfurt a. M., Germany). All standards were certified reference material and had a purity of at least 99.0%, except for THC-OH-d3, THCAA, CBC, CBC-d9, CBCA, CBL, THCVA, CBDV and CBDVA (97.7%), THC-d3 (96.7%), and CBG-d9 (95.0%). All chemicals were obtained from Carl Roth (Karlsruhe, Germany), except for methanol (from Fisher Scientific (Loughborough, UK)) and acetonitrile (from AppliChem (Darmstadt, Germany)). Chemicals used in LC-MS/MS measurement processes were LC-MS grade. All other chemicals were HPLC grade, except for acetone and dichloromethane, which had a purity of 99.8% and 99.5%, respectively. Blank human serum used for calibration was obtained from voluntary healthy blood donors via blood donation service and was tested to be drug-free.

Scheunemann A., Elsner K., Germerott T., Groppa S., Hess C., Miederer I., Poplawski A, & Röhrich J. (2021). Identification of Potential Distinguishing Markers for the Use of Cannabis-Based Medicines or Street Cannabis in Serum Samples. Metabolites, 11(5), 316.

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Quantification of Plasma THC Levels

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Blood samples were collected (~500 ul) via jugular needle insertion under anesthesia with an isoflurane/oxygen vapor mixture (isoflurane 5% induction, 1-3% maintenance) 35 minutes post-initiation of vapor inhalation. Plasma THC content was quantified using fast liquid chromatography/mass spectrometry (LC/MS) adapted from (Irimia et al., 2015 (link); Lacroix and Saussereau, 2012 ; Nguyen et al., 2017 (link)). 5 μL of plasma were mixed with 50 μL of deuterated internal standard (100 ng/mL CBD-d3 and THC-d3; Cerilliant), and cannabinoids were extracted into 300 μL acetonitrile and 600 μL of chloroform and then dried. Samples were reconstituted in 100 μL of an acetonitrile/methanol/water (2:1:1) mixture. Separation was performed on an Agilent LC1100 using an Eclipse XDB-C18 column (3.5um, 2.1mm x 100mm) using gradient elution with water and methanol, both with 0.2 % formic acid (300 μL/min; 73-90%). Cannabinoids were quantified using an Agilent MSD6140 single quadrupole using electrospray ionization and selected ion monitoring [CBD (m/z=315.2), CBD-d3 (m/z=318.3), THC (m/z=315.2) and THC-d3 (m/z=318.3)]. Calibration curves were conducted for each assay at a concentration range of 0-200 ng/mL and observed correlation coefficients were 0.999.

Nguyen J.D., Grant Y., Kerr T.M., Gutierrez A., Cole M, & Taffe M.A. (2018). Tolerance to hypothermic and antinoceptive effects of Δ9-tetrahydrocannabinol (THC) vapor inhalation in rats. Pharmacology, biochemistry, and behavior, 172, 33-38.

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Quantifying Plasma THC Levels by LC/MS

Cited 1 time

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Blood samples were collected (~500 μl) via jugular needle insertion following anesthesia with an isoflurane/oxygen vapor mixture (isoflurane 5% induction). Plasma THC content was quantified using fast liquid chromatography/mass spectrometry (LC/MS) adapted from (Irimia, Polis, Stouffer, & Parsons, 2015 (link); Lacroix & Saussereau, 2012 (link); Nguyen et al., 2018 (link)). 50 μl of plasma were mixed with 50 μl of deuterated internal standard (100 ng/ml CBD-d3 and THC-d3; Cerilliant), and cannabinoids were extracted into 300 μL acetonitrile and 600 μl of chloroform and then dried. Samples were reconstituted in 100 μl of an acetonitrile/methanol/water (2:1:1). Separation was performed on an Agilent LC1100 using an Eclipse XDB-C18 column (3.5um, 2.1mm × 100mm) using gradient elution with water and methanol, both with 0.2 % formic acid (300 μl/min; 73–90%). Cannabinoids were quantified using an Agilent MSD6140 single quadrodpole using electrospray ionization and selected ion monitoring [CBD (m/z=315.2), CBD-d3 (m/z=318.2), THC (m/z=315.2) and THC-d3 (m/z=318.2)]. Calibration curves were conducted daily for each assay at a concentration range of 0–200 ng/mL and observed correlation coefficients were 0.999.

Taffe M.A., Creehan K.M., Vandewater S.A., Kerr T.M, & Cole M. (2020). Effects of Δ9-tetrahydrocannabinol (THC) vapor inhalation in Sprague-Dawley and Wistar rats. Experimental and clinical psychopharmacology, 29(1), 1-13.

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Quantification of Cannabinoids in Biological Matrices

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THC, CBD, THC-d3 and CBD-d3 standards were acquired from Cerilliant (Round Rock, TX, USA). Methanol and acetonitrile—Optima® LC/MS Grade, ammonium formate and formic acid were purchased from Fisher Scientific (Waltham, MA, USA). Ultrapure water was obtained using a Direct-Q 3UV system of Millipore (Burlington, MA, USA). Whatman 903® paper was acquired from GE Healthcare Life Sciences (Marlborough, MA, USA) and WAX-S tips (300µL Hamilton 2 mg WAX + 10 mg salt) were purchased from DPX technologies (Columbia, SC, USA).

Gorziza R., Cox J., Limberger R.P, & Arroyo-Mora L.E. (2021). Study of Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) extraction FROM dried oral fluid spots (DOFS) and LC–MS/MS detection. Journal of Cannabis Research, 3, 30.

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Certified Reference Material Protocol

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Certified reference material for THC, CBD, THCA-A, CBDA, CBN, THC-d3, CBD-d3, and CBN-d3 was purchased from Cerilliant Corporation (Round Rock, TX). All standards were analytical grade and were provided as either 1 mg/mL or 100 μg/mL (THC-d3, CBD-d3, CBN-d3) solution in methanol or acetonitrile. All solvents used were HPLC grade and purchased from Sigma-Aldrich (Missouri, USA).

Lindsay C.M., Abel W.D., Jones-Edwards E.E., Brown P.D., Bernard K.K, & Taylor T.T. (2021). Form and content of Jamaican cannabis edibles. Journal of Cannabis Research, 3, 29.

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Quantification of Plasma THC Levels

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Blood samples were collected (~300–500 μl) via jugular needle insertion following anesthesia with an isoflurane/oxygen vapor mixture (isoflurane 5% induction) or via implanted catheter for groups prepared for i.v. sampling. Plasma THC content was quantified using fast liquid chromatography/mass spectrometry (LC/MS) adapted from (Irimia, Polis, Stouffer, & Parsons, 2015; Lacroix & Saussereau, 2012; Nguyen et al., 2018 (link)). 50 μl of plasma were mixed with 50 μl of deuterated internal standard (100 ng/ml CBD-d3 and THC-d3; Cerilliant), and cannabinoids were extracted into 300 μL acetonitrile and 600 μl of chloroform and then dried. Samples were reconstituted in 100 μl of an acetonitrile/methanol/water (2:1:1). Separation was performed on an Agilent LC1100 using an Eclipse XDB-C18 column (3.5um, 2.1mm × 100mm) using gradient elution with water and methanol, both with 0.2 % formic acid (300 μl/min; 73–90%). Cannabinoids were quantified using an Agilent MSD6140 single quadrodpole using electrospray ionization and selected ion monitoring [CBD (m/z=315.2), CBD-d3 (m/z=318.2), THC (m/z=315.2) and THC-d3 (m/z=318.2)]. Calibration curves were conducted daily for each assay at a concentration range of 0–200 ng/mL and observed correlation coefficients were 0.999.

Nguyen J.D., Creehan K.M., Grant Y., Vandewater S.A., Kerr T.M, & Taffe M.A. (2020). Explication of CB1 receptor contributions to the hypothermic effects of Δ9-tetrahydrocannabinol (THC) when delivered by vapor inhalation or parenteral injection in rats. Drug and alcohol dependence, 214, 108166.

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Cannabinoid Standardization and Analysis

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CBN (1 mg/mL in methanol, certified reference material), CBN-d₃ (100 μg/mL in methanol, certified reference material), CBD (1 mg/mL in methanol, certified reference material), and CBD-d₃ (100 μg/mL in methanol, certified reference material) were bought from Cerilliant; acetonitrile (HPLC gradient grade), methanol (HPLC gradient grade), n-hexane (HPLC grade), n-heptane (HPLC grade), and formic acid (98–100%) were obtained from VWR Chemicals; dichloromethane (HPLC grade) from Carl Roth, triethylamine from ACROS Organics, Fast Blue Salt B (FBS, dye content ~ 95%) from Sigma-Aldrich, Chromabond SiOH (1 ml/100 mg), Chromabond C₁₈ ec (1 ml/ 100 mg) as well as TLC (thin-layer chromatography) plates (silica gel 60, ALUGRAM Xtra SIL G UV₂₅₄ and octadecyl-modified silica, ALUGRAM RP-18 W/UV₂₅₄) from Macherey-Nagel, TLC plates (silica gel 60 without fluorescent indicator on aluminum sheets), and HPTLC (high-performance thin-layer chromatography) plates (silica gel 60 F₂₅₄ MS-grade for matrix-assisted laser desorption/ionization (MALDI) and silica gel 60 F₂₅₄ on glass plates) were purchased from Merck KGaA and analytical sea sand from Grüssig GmbH. Distilled diethyl ether and acetone were produced with a rotary evaporator from BÜCHI.

Schmidt T., Kramell A.E., Oehler F., Kluge R., Demske D., Tarasov P.E, & Csuk R. (2020). Identification and quantification of cannabinol as a biomarker for local hemp retting in an ancient sedimentary record by HPTLC-ESI-MS. Analytical and Bioanalytical Chemistry, 412(11), 2633-2644.

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Certified Reference Materials for Cannabinoid Analysis

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Certified reference material
stock solutions of native THC, COOH-THC, OH-THC, CBD, and CBN and
their isotopically labeled counterparts, THC-d₃, COOH-THC-d₃, OH-THC-d₃, CBD-d₃, and CBN-d₃, were purchased from Cerilliant Corporation
(Round Rock, TX). Native COOH-CBD was purchased from Toronto Research
Chemicals (North York, ON, Canada). Acetonitrile (HPLC grade), methanol
(HPLC grade), formic acid (≥99.5%), ammonium formate (≥99%),
and phosphate-buffered saline were purchased from Fisher Scientific
(Pittsburgh, PA). Water (HPLC grade) was purchased from JT Baker (Phillipsburg,
NJ).

Brosius C.R., Caron K.T., Sosnoff C.S., Blount B.C, & Wang L. (2021). Rapid Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method to Measure Cannabinoids in Bronchoalveolar-Lavage Fluid of Patients with e-Cigarette, or Vaping, Product Use-Associated Lung Injury. ACS Omega, 7(1), 443-452.

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Cannabinoid Quantification Using LC-MS

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We purchased high-purity methanol (≥99.9%), 2-propanol (≥99.9%), formic acid (≥99.5%), and ammonium formate (≥99%) from Fisher Scientific (Fair Lawn, NJ). We purchased liquid chromatography–mass spectrometry (LC–MS) grade water (J.T. Baker, ≥99.9%) and acetonitrile (Burdick & Jackson, ≥99.9%) from VWR (Radnor, PA). We purchased ammonium sulfate, sodium hydroxide (NaOH), ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA-Na₂), and 0.5 M dibutylammonium acetate from Sigma-Aldrich Laboratories, Inc. (St. Louis, MO). We purchased native and isotopically labeled standards, including THC, THC-d₃, CBD, CBD-d₃, CBN, and CBN-d₃ from Cerilliant (Round Rock, TX). We bought the SPE (C18, 100 mg) column and 96-well plate from Phenomenex (Torrance, CA). All chemicals were used without further purification.

Wei B., McGuffey J.E., Blount B.C, & Wang L. (2016). Sensitive Quantification of Cannabinoids in Milk by Alkaline Saponification–Solid Phase Extraction Combined with Isotope Dilution UPLC–MS/MS. ACS Omega, 1(6), 1307-1313.

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