Anti mybbp1a

Western blots were performed as previously described elsewhere. Membranes were incubated with the following primary antibodies: anti‐MYBBP1A (Proteintech #14524‐AP, Rosemont, IL, USA ), anti‐PGC1α (Abcam #ab54481, Cambridge, UK), anti‐SGLT1 (Abcam #ab14685), anti‐p38 MAPK (Cell Signaling #9212, Danvers, MA, USA), and anti‐phospho‐p38 MAPK (T180/Y182) (Cell Signaling #9215). α‐Tubulin (Sigma #T9026) was used as a loading control. Horseradish peroxidase‐labeled rabbit anti‐mouse (Abcam #ab 97046) and goat anti‐rabbit (Abcam #ab 97051) secondary antibodies were used. The proteins were detected using an ECL detection system (Amersham Biosciences) and Bio‐Rad ChemiDoc XRST (Berkeley, CA, USA).

Western blots were performed as previously described elsewhere. Membranes were incubated with the following primary antibodies: anti-MYBBP1A (Proteintech #14524-AP), anti-c-MYB (Millipore #05-175, Billerica, MA, USA), anti-pVHL (Santa Cruz # sc-5575, Santa Cruz, CA, USA), anti-p53 (Santa Cruz #sc-6243), anti-acetyl-p53 (Lys 382) (Cell Signaling #2525, Danvers, MA, USA). a-Tubulin (1:10,000, Sigma #T9026) was used as a loading control. Horseradish peroxidase-labeled rabbit anti-mouse (Abcam # ab 97046) and goat anti-rabbit (Abcam # ab 97051) secondary antibodies were used. The proteins were detected using an ECL detection system (Amersham Biosciences, GE Healthcare, Buckinghamshire, UK) and Bio-Rad ChemiDoc XRST (Hercules, CA, USA).