Isotype matched control igg

Manufactured by Thermo Fisher Scientific

Isotype-matched control IgG is a laboratory reagent used as a control in immunological experiments. It is a purified immunoglobulin (IgG) that matches the isotype of the primary antibody being used in the experiment, but does not bind to the target antigen.

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Lab products found in correlation

Anti f4 80, by Thermo Fisher Scientific (2 mentions) F4 80, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Isotype‐matched IgG Isotype control IgG Isotype controls Isotype IgG Control IgG

3 protocols using isotype matched control igg

Immunohistochemical Analysis of Skin and Lung Tissue

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We analyzed 4 μm sections of FFPE skin biopsy samples from patients with SSc, healthy individuals, or from mouse skin tissue and lungs by IHC, as previously described (65 (link)). Slides were incubated with anti-mouse CD38 (Abcam; catalog ab230153), anti-mouse or human ASMA (MilliporeSigma; catalog A2547), F4/80 (Cell Signaling; catalog 70076T), anti-human MARCO (Abcam; catalog ab231046), anti-mouse MARCO (Abcam; catalog ab256822), and mouse CD68 (Abcam; catalog ab125212) Abs or isotype-matched control IgG (eBioscience), followed by HRP-conjugated secondary Ab, which was visualized with diaminobenzidine substrate and counterstained with hematoxylin. Immunopositive cells were counted in 5 randomly selected high-power fields (original magnification, ×40) per biopsy specimen by an observer in a blinded manner. One-way ANOVA was used for analysis of statistical significance.

Xu D., Bhattacharyya S., Wang W., Ifergan I., Wong M.Y., Procissi D., Yeldandi A., Bale S., Marangoni R.G., Horbinski C., Miller S.D, & Varga J. (2022). PLG nanoparticles target fibroblasts and MARCO+ monocytes to reverse multiorgan fibrosis. JCI Insight, 7(5), e151037.

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Arthritis Evaluation in CD11c-Flip-KO Mice

Cited 1 time

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CD11c-Flip-KO and the littermates were assessed for the spontaneous development of arthritis on a weekly basis from 4 weeks to 36 weeks of age. The clinical severity score was defined as the sum (maximum=28) of joint swelling/inflammation (0–3 each of 4 paws/ankles), joint deformity (including toe flexion, contraction and shortening, 0–3 of each of 4 paws/ankles) and the grip strength (on a 3 mm diameter of wires, 0–4/mouse)^{57 (link)}. The clinical incidence was defined as any mouse with clinically observed joint swelling/inflammation ≥ 1. The histologic analysis was performed utilizing paraffin embedded sections of interphalangeal joints of the back and front paws, ankles, and knees subjected to hematoxylin and eosin (H&E) staining⁵⁸. H&E ankle sections were evaluated by a blinded pathologist for inflammation (0–5), bone erosion (0–5), pannus formation (0–5) and median synovial lining thickness, neutrophil infiltration, and cartilage destruction^{58 , 59 (link)}. Macrophages in ankles were identified by immunohistochemistry staining by the Northwestern University Mouse Histology & Phenotyping Laboratory employing anti-F4/80 (eBioscience, cat. no. 14-4801, 1:1000) or isotype matched control IgG (eBioscience, cat. no. 16-4321) antibodies.

Huang Q.Q., Perlman H., Birkett R., Doyle R., Fang D., Haines G.K., Robinson W., Datta S., Huang Z., Li Q.Z., Phee H, & Pope R.M. (2015). CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nature Communications, 6, 7086.

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Spontaneous Arthritis Development in CD11c-Flip-KO Mice

Cited 2 times

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CD11c-Flip-KO and the littermates were assessed for the spontaneous development of arthritis on a weekly basis from 4 to 36 weeks of age. The clinical severity score was defined as the sum (maximum=28) of joint swelling/inflammation (0–3 each of four paws/ankles), joint deformity (including toe flexion, contraction and shortening, 0–3 of each of four paws/ankles) and the grip strength (on a 3-mm diameter of wires, 0–4 per mouse)57 (link). The clinical incidence was defined as any mouse with clinically observed joint swelling/inflammation ≥1. The histologic analysis was performed utilizing paraffin-embedded sections of interphalangeal joints of the back and front paws, ankles and knees subjected to haematoxylin and eosin (H&E) staining58 (link). H&E ankle sections were evaluated by a blinded pathologist for inflammation (0–5), bone erosion (0–5), pannus formation (0–5) and median synovial lining thickness, neutrophil infiltration and cartilage destruction58 (link)59 (link). Macrophages in ankles were identified with immunohistochemistry staining by the Northwestern University Mouse Histology & Phenotyping Laboratory employing anti-F4/80 (eBioscience, cat. no. 14–4801, 1:1,000) or isotype-matched control IgG (eBioscience, cat. no. 16–4321) antibodies.

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