Sodium selenite, indomethacin, β-cyclodextrin (β-CD), Pluronic® F127 (PF-127), soybean lecithin, bovine serum albumin (BSA), carrageenan and glutathione (GSH) were purchased from Sigma (St Louis, MO, USA). Polyethylene glycol (PEG 6000) was purchased from Adwic, EL-Nasr Pharmaceutical Chemicals (Cairo, Egypt). All other chemicals and solvents used were of analytical grade.
Soybean lecithin
Manufactured by Merck Group
Sourced in United States, Germany
Soybean lecithin is a natural emulsifier derived from soybeans. It is a complex mixture of acetylated phosphatides, consisting chiefly of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Soybean lecithin functions as a surfactant, emulsifier, and stabilizer in various food, pharmaceutical, and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Cholesterol, by Merck Group (3 mentions)
Dodab, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Sodium deoxycholate, by Merck Group (2 mentions)
Stearic acid, by Merck Group (2 mentions)
N hydroxysuccinimide, by Merck Group (2 mentions)
Agar agar, by Merck Group (2 mentions)
Sodium alginate, by Merck Group (2 mentions)
Glycerol, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Carrageenan, by Merck Group (1 mentions)
β cyclodextrin β cd, by Merck Group (1 mentions)
Pluronic f127 pf127, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Luria bertani broth, by BD (1 mentions)
25 protocols using soybean lecithin
1
Formulation and Evaluation of Selenite Nanoparticles
2
Protein Purification Protocol
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Sucrose, tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), soybean lecithin (L-α-phosphadidylcholine ≥30%, P3644), imidazole, ampicillin, sodium chloride (NaCl), and monosodium phosphate (NaH2PO4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luria-Bertani (LB) broth was obtained from Becton, Dickinson and Company (Franklin Lakes, NJ, USA). Ni-NTA Superflow resin was acquired from Qiagen Inc. (Valencia, CA, USA).
3
In Vitro and In Vivo Anticancer Evaluation
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Hydrogen chloride, MTT, PBS, soybean lecithin, cholesterol, sodium hydroxide, mannitol, H&E, dialysis bag (cut-off of 10,000 Da), ethanol (EtOH), xylazine, ketamine hydrochloride, and Cispt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Roswell Park Memorial Institute (RPMI)-1640 medium, penicillin and streptomycin antibiotics, and fetal bovine serum (FBS) were purchased from Gibco (Waltham, MA, USA). BBN was purchased from J&K Scientific Co., Ltd. (Beijing, China). PEG2000 was purchased from Kimiagaran Emrooz Chemical Ind. (Arak, Iran). Female Wistar rats (8 weeks, 250 g) and bladder carcinoma cell line (HTB-9) were supplied by Pasteur Institute of Iran, Tehran. All other materials were of analytical grade. Deionized water was used throughout the study.
4
Synthesis and Characterization of Polymeric Nanoparticles
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MMA, PDDA, azobisisobutyronitrile (AIBN), NaCl, CTAB, DODAB, soybean lecithin, chloroform, ethanol, dichloromethane, and Mueller–Hinton agar (MHA) were purchased from Sigma-Aldrich (Darmstadt, Germany) and used without further purification. The composition of soybean lecithin includes several fatty acids and phospholipids [31 (link),32 (link)]. Silicon (100) wafers were from Silicon Quest (Santa Clara, CA, USA) with a native oxide layer approximately 2 nm thick and used as substrates for casting the dispersions. These Si wafers with a native SiO2 layer were cut into small pieces of ca 1 cm2, cleaned with acetone, and dried under a N2 stream; they are smooth substrates for the coatings. The syntheses in 1 mM NaCl solution prepared with Milli-Q water yielded NPs dispersions by emulsion polymerization that underwent dialysis for purification using a cellulose acetate dialysis bag with molecular weight cut-off around 12,400 g/mol. All other reagents were analytical grade and used without further purification.
5
Formulation and Characterization of Lipid-based Nanocarriers
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VRC, DGG and soybean lecithin were procured from Sigma Aldrich (St, Louis, MO, USA). CO was purchased from Avanti Polar Lipids, Inc. (Birmingham, AL, USA). Tween 80 was generously gifted by the Saudi Drugs and Medical Instruments Company, Jeddah, Saudi Arabia. Solvents of a grade suitable for high-performance liquid chromatography were collected from the Merck Group (Darmstadt, Germany). All other reagents and chemicals used were of analytical grade.
6
Quercetin Nanoformulation and Characterization
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Quercetin (QER), methanol (HPLC grade), soybean lecithin, sodium deoxycholate (SDC), poloxamer 407 (PF407), chloroform (HPLC grade), poloxamer 188 (PF188), and carbopol 971P (CP971P), were procured from Sigma-Aldrich (St. Louis, MO, USA). A primary polyclonal rabbit antibody against the c-fos protein was obtained from Cambridge Research Biochemicals (Cambridge, UK), CRB OA-11-823, with a 0.1% Triton dilution in PBS at 1:10,000). A secondary anti-rabbit antibody (1:400) was obtained from Vector Labs (Burlingame, CA, USA). All other ingredients and chemicals utilized were of analytical quality.
7
Synthesis and Characterization of Multifunctional Nanoparticles
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Stearic acid, lauric acid, soybean lecithin, sodium hyaluronate, N-hydroxysuccinimide (NHS), sodium periodate (NaIO4), and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDCI) were purchased from Sigma-Aldrich (America). DSPE-mPEG2000 was obtained from Advanced Vehicle Technology Pharmaceutical Tech Co., Ltd (Shanghai, China). RGX-104 was provided by RayStarBio (Hangzhou, China). Methyl 3-mercapto propionate, dopamine hydrochloride, gamma polyglutamic acid (PGA), β-mercaptoethanol and 2-(N-Morpholino)ethanesulfonic acid (MES) were purchased from Macklin (Shanghai, China). Recombinant mouse GM-CSF and IL-4 were obtained from Peprotech (Rocky Hill, NJ, USA). Fetal Bovine Serum was purchased from ExCell Bio (cat: FSP500; Shanghai, China) and Haixing Bioscience (cat: FBP-C520; Suzhou, China). Mouse IL-6, TGF-β, and IL-10 ELISA kits were acquired from Biolegend (America), and mouse IFN-γ ELISA kits were acquired from Invitrogen (America). Anti-CD3-PE-Cy7, anti-CD4-FTIC, anti-CD4-APC, anti-CD8α-APC, anti-CD8α-FITC, anti-CD69-PE, anti–IFN–γ-PE, anti-Granzyme B-Pacific Blue, anti-CD44-FITC, anti-CD62L-APC, anti-DX5-PE, anti-CD11c-FITC, anti-CD11c-PE, anti-CD103-APC, anti-CD11b-PE, anti-CD11b-FITC, anti-F4/80-PE, anti-CD80-APC, anti-CD86-PE-Cy7, anti-CD206-APC, anti-Gr-1-PE, anti-CD25-FITC, anti-Foxp3-APC, anti-CD45-APC were purchased from Biolegend (America).
8
Recombinant Phospholipase D Expression
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E. coli BL21(DE3) and pET-28a(+) (Novagen, China) were applied for gene expression. The recombinant plasmid pET28a(+)-PLD was constructed and preserved in our laboratory. E. coli BL21(DE3) were cultured in a Luria-Bertani medium comprising (g/L): tryptone 10, yeast extract 5 and NaCl 10, pH 7.2. Soybean lecithin(Sigma, China) and L- serine(Aladdin, China) were used as substrates for the synthesis of PS. Phosphatidylserine(Sigma, China) were purchased to be the standard. All other chemicals and reagents used were of analytical grade.
9
Iron Chelation and Antioxidant Evaluation
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The standard iron chelating drug, deferoxamine (DFO) was obtained from Novartis International AG (Basel, Switzerland). Carbonyl iron, GA, soybean lecithin, ethanol, vitamin C, ferric chloride, ferrous chloride, linoleic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 2-azinobis (3-ethylbenzo-thiozoline-6-sulfonic acid) (ABTS), potassium ferricyanide and 30% hydrogen peroxide were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The assay kits for alanine transaminase (ALT) (C009-2-1), aspartate transaminase (AST) (C010-2-1), γ-glutamyl transferase (γ-GT) (C017-2-1), and superoxide dismutase (SOD) (A001-3-2), glutathione (GSH) (A006-2-1), malondialdehyde (MDA) (A003-1-1), total cholesterol (TC) (A111-1-1), total iron-binding capacity (TIBC) (A040-1-1), triglyceride (TG) (A110-1-1) were procured from Nanjing Jiancheng Biotechnology Co., Ltd. (Nanjing, China). Antibodies against transferrin receptor (TfR1) (ab84036), Ferritin (ab153976), ferritin light chain (FL) (ab109373) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (ab198306) were all purchased from Abcam Inc. (Cambridge, MA, USA).
10
Preparation of Giant Unilamellar Vesicles
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Giant Unilamellar Vesicles (GUVs) were used as models of cell membranes and were prepared using a procedure previously described [26 (link)]. First, by evaporation under a nitrogen flow, we prepared a film of 1 mM L-α-phosphatidylcholine (Soybean lecithin, from Sigma-Aldrich, St. Louis, MO, USA). After that, 20 µL of water was added to the film, and the resulted solution was kept at 45 °C for 45 min. Then, 3 mL of 0.1 M glucose solution was added, and the mixture was incubated at 37 °C for 2 h. Finally, GUVs solution was centrifuged at 14,000× g for 30 min at 20 °C to remove lipid aggregates and multilamellar vesicles (pellet).
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