Il 1α and il 1β duoset

The cell culture medium LHC-9, DMEM:F12 and Trypsin–EDTA were bought from Gibco, Thermo Fisher Scientific, Waltham, MA, USA. The cell culture flasks were obtained from Nunc A/S, Roskilde, Denmark, while the cell culture plates were from Corning, NY 14831 USA. PureCol collagen was from Advanced BioMatrix, Inc, CA, USA. The Cytotoxicity Detection Kit using lactate dehydrogenase (LDH) activity was bought from Merck KGaA, Darmstadt, Germany. AlamarBlue™ Cell Viability Reagent and the sandwich enzyme-linked immunosorbent assay (ELISA) cytosets for CXCL8 and IL-6 were purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, while IL-1α and IL-1β DuoSet were from R&D Systems, Inc, Minneapolis, MN, USA. The Endotoxin Quant kit were from Thermo Fisher Scientific, Waltham, MA USA. For measurements of acellular ROS 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), Dulbecco’s PBS; hydrogen peroxide (H₂O₂), dithiothreitol (DTT) and ultrapure H₂O were purchased from Merck KGaA, Darmstadt, Germany. Whatman TE 38 filter was from (Whatman, Maidstone, Kent, UK). Other chemicals were purchased from commercial sources at the highest purity available.

The cell culture medium LHC-9 and DMEM:F12, and Trypsin-EDTA, were bought from Gibco, Thermo Fisher Scientific, Waltham, MA USA. The cell culture flasks were obtained from Nunc A/S, Roskilde, Denmark, while the cell culture plates were from Corning, NY 14,831 USA. PureCol^™ collagen was from Advanced BioMatrix, Inc, CA, USA.The ELISA cytoset for CXCL8 was purchased from Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, while IL-1α and IL-1β DuoSet were from R&D Systems, Inc, Minneapolis, MN, USA. Cytotoxicity Detection Kit (lactate dehydrogenase; LDH) was obtained from Merck KGaA, Darmstadt, Germany. The RNA isolating kit; NukleoSpin RNA plus, was bought from MACHEREY-NAGEL, Duren, Germany. CH22319 and N-acetyl cystein (NAC) were bought from Merck KGaA, Darmstadt, Germany. High Capacity cDNA Archive Kit, TaqMan Universal PCR Mastermix, TaqMan Gene Expression Assays (CXCL8, Hs00174103_m1, IL-1α; Hs00174092_m1, IL-1β; Hs01555410_m1, TNF-α; Hs01113624_g1, HO-1; Hs01110250_m1, CYP1A1; Hs01054797_g1, CYP1B1; Hs02382916_s1, PAI-2 ; Hs01010736_m1, COX2; Hs00153133_m1 and GAPDH; Hs02758991_g1) were bought from Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA. Other chemicals were purchased from commercial sources at the highest purity available.