125i α bungarotoxin 125i α btx

[¹²⁵I]α-Bungarotoxin ([¹²⁵I]α-BTX) (210–250 Ci·mmol⁻¹) and the scintillation solution (Ultima Gold F) were purchased from PerkinElmer (Courtaboeuf, France). Ethyl-3-amino benzoate methanesulfonate, ethylene diamine tetraacetic acid (EDTA), acetylcholine chloride, tricaine, and other chemicals were from Sigma-Aldrich (Saint Quentin Fallavier, France), or other standard sources. The α-cobratoxin from Naja kaouthia was obtained by recombinant expression, refolded, and purified in our laboratory, as described previously (see [57 (link)]).

20-methyl spirolide G (≥97% purity), obtained from laboratory cultures of the dinoflagellate Alexandrium ostenfeldii, was purchased from Cifga (Lugo, Spain). [¹²⁵I]α−Bungarotoxin ([¹²⁵I]α−BTX) (210–250 Ci·mmol−1), and (±)[³H]epibatidine (55 Ci·mmol⁻¹), were purchased from PerkinElmer (Courtaboeuf, France). Isoflurane (AErrane^®) was purchased from Baxter S.A. (Lessines, Belgium). Other chemicals were obtained from Sigma-Aldrich (Saint Quentin Fallavier, France), or other standard sources. The α-toxin from Naja nigricollis was synthesized, refolded, and purified, as previously described [30 (link)]. Methyllycaconitine (MLA), and (±)epibatidine were purchased from Tocris Bioscience (Bristol, UK).