UV and IR spectra were measured on an UV-2401PC spectrometer (Shimadzu, Beijing, China) and IR Affinity-1 spectrometer (Shimadzu, Beijing, China), respectively. Optical rotations were performed on a Perkine Elmer 341 polarimeter, and CD spectra were measured with a chirascan circular dichroism spectrometer (Applied Photophysics, Surrey, UK). HR-ESIMS were determined with a Bruker maXis Q-TOF in positive/negative ion mode. The NMR spectra including (1D and 2D NMR) were recorded on a Bruker AC 500 MHz spectrometer using TMS as standard. All chemical shifts were assigned with δ-values. X-ray diffraction intensity data were collected on Agilent Xcalibur Nova single-crystal diffractometer using Cu Kα radiation. Column chromatography (CC) was performed on silica gel (200–300 mesh, 300–400 mesh), and Sephadex LH-20 (Amersham Biosciences, Sweden), respectively. TLC were carried out on silica gel GF254 (10–40 µm) plates (Qingdao Marine Chemical Factory, China). All solvents used were of analytical grade (Tianjin Fuyu Chemical and Industry Factory). Semipreparative HPLC (Agilent Technologies, 1260 infinity series) was performed using an ODS column (YMC-pack ODS-A, 10 × 250 mm, 5 µm, 1.5 mL/min).
Uv 2401pc spectrometer
Manufactured by Shimadzu
Sourced in Japan
The UV-2401PC spectrometer is a high-performance UV-Vis spectrophotometer designed for analytical applications. It features a wide wavelength range, high-resolution optics, and advanced data processing capabilities. The instrument is capable of performing accurate measurements of absorption, transmittance, and reflectance spectra across a variety of samples.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel, by Qingdao Marine Chemical (12 mentions)
Gf254, by Qingdao Marine Chemical (8 mentions)
Ft ir tensor 27 infrared spectrophotometer, by Bruker (7 mentions)
Sephadex lh 20, by Cytiva (5 mentions)
Mci gel, by Mitsubishi Chemical Corporation (5 mentions)
P 1020 polarimeter, by Jasco (4 mentions)
Sepa 300 polarimeter, by Horiba (4 mentions)
Mci gel chp 20p, by Mitsubishi (4 mentions)
Drx 600 spectrometer, by Bruker (4 mentions)
Xevo tq s, by Waters Corporation (3 mentions)
Autospec premier p776, by Waters Corporation (3 mentions)
Avance 3 600 mhz spectrometer, by Bruker (3 mentions)
Xevo tqs, by Agilent Technologies (3 mentions)
Drx 600, by Bruker (2 mentions)
Sb c18, by Agilent Technologies (2 mentions)
Drx 500 nmr, by Bruker (2 mentions)
P 1020 automatic digital polariscope, by Jasco (2 mentions)
G6230 tof mass spectrometer, by Agilent Technologies (2 mentions)
P 1020 digital, by Horiba (2 mentions)
Zorbax sb c18 column, by Agilent Technologies (2 mentions)
29 protocols using uv 2401pc spectrometer
1
Comprehensive Structural Elucidation Protocol
2
Optimizing UV Exposure for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optimal UV exposure time was determined using CellTiter-Blue® cell viability assay (Promega Corporation, USA). Cells were grown to 70–80% confluency in 100 mm culture plates and synchronized by overnight serum starvation. Viability assay was performed by adding dye in 1:5 ratio to the culture medium. Cells were incubated in the dark for ~4 hours and absorbance was recorded using UV-2401 PC spectrometer (Shimadzu). 570 and 600 nm were chosen as reference wavelengths, and blank samples consisted of only CellTiter-Blue reagent without cells. Culture medium background at 600 nm was subtracted from experimental values at 570 nm and plot of this difference against the UVR exposure time was generated. Three different time points where cells did not have any structural damage but had significant differences in the absorbance value of the reduced dye product were chosen for further experiments.
3
Spectroscopic Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 1D and 2D NMR spectra were recorded on a Bruker DPX 400 instrument (Bruker, Bremen, Germany) with tetramethylsilane as the internal standard and MeOH-d4 as the solvent. The HR-ESI-MS experiments were performed on a Waters Xevo G2-S QTOF (Waters Corporation, Milford, MA, USA). The semi-preparative HPLC procedure was performed on a Shimadzu LC-16P instrument with a RID-20A (Shimadzu Tokyo, Japan) and a reversed-phase C18 column (250 × 10 mm, 5 µm, Shim-pack GIST, Shimadzu Tokyo, Japan)). UV spectra were scanned with a SHIMADZU UV-2401PC spectrometer (Shimadzu Tokyo, Japan). Infrared spectra were performed on a VERTEX 70 spectrometer (Bruker, Bremen, Germany) using KBr particles. The rotational luminosity was measured on an Autopol VI instrument. Electron circular dichroism spectra were recorded on a LAAPD detector. Column chromatography was performed with silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China) and octadecyl silica gel (ODS) (50 µm, Merck, Darmstadt, Germany).
4
Comprehensive Analytical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were measured on a Jasco P-1020 polarimeter. UV spectra were detected on a Shimadzu UV-2401PC spectrometer. IR spectra were determined on a Bruker FT-IR Tensor-27 infrared spectrophotometer with KBr disks. 1D and 2D NMR spectra were recorded on DRX-500 spectrometers using TMS as an internal standard. Unless otherwise specified, chemical shifts (δ) were expressed in ppm with reference to the solvent signals. ESIMS and HR-EIMS analysis were carried out on Waters Xevo TQS and Waters AutoSpec Premier P776 mass spectrometers, respectively. Semi-preparative HPLC was performed on an Agile 1100 HPLC with a Zorbax SB-C18 (9.4 × 250 mm) column. Silica gel (100–200 and 200–300 mesh, Qingdao Marine Chemical Co., Ltd., PR China), and Amphichroic RP-18 gel (40–63 μm, Merck, Darmstadt, Germany) and MCI gel (75–150 μm, Mitsubishi Chemical Corporation, Tokyo, Japan) were used for column chromatography.
5
Compound 5 Protein Binding Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ground-state absorption spectra were recorded with a Shimadzu UV-2401PC Spectrometer. 1 cm path-length quartz cuvette was used and 250–450 nm wavelength range was scanned. Compound 5 absorption spectra as a function of BSA and HSA concentration were recorded by titrating (Banerjee et al. 2012 (link); Ray et al. 2012 (link)) compound 5 solution with concentrated protein solutions. Small dilution error in the titration experiment was ignored.
6
Analytical Techniques for Natural Product Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Column chromatography was undertaken on MCI gel CHP 20P (75–150 μm, Mitsubishi Chemical Industries, Tokyo, Japan), Silica gel (200–300 mesh, Qingdao Marine Chemical Inc., Qingdao, China), RP-18 (40–60 µm; Daiso Co., Tokyo, Japan), and Sephadex LH-20 (Amersham Pharmacia, Uppsala, Sweden). Optical rotations were measured on a Horiba SEPA-300 polarimeter (Horiba, Kyoto, Japan). UV spectra were obtained on a Shimadzu UV-2401PC spectrometer (Shimadzu Corporation, Tokyo, Japan). GC analysis was performed using an Agilent 6890N gas chromatography instrument (Agilent Technologies, Santa Clara, CA, USA). Semi-preparative or analytic HPLC was carried out using an Agilent 1200 liquid chromatograph (Agilent Technologies, Santa Clara, CA, USA) where the columns used were a YMC-Pack ODS-A (250 mm × 9.4 mm, i.d., 5 µm), or an Agilent Eclipse XDB-C18 (150 mm × 4.6 mm, i.d., 5 µm). NMR spectra were recorded at room temperature on an AV-400 or AV-600 spectrometer (Bruker, Karlsruhe, Germany) with TMS as an internal standard. ESIMS and HRESIMS data were collected by an Agilent G6230TOF MS spectrometer (Agilent Technologies, Santa Clara, CA, USA).
7
Purification and Characterization of Natural Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were performed on a JASCO P-1020 digital polarimeter. UV spectra were obtained on a Shimadzu UV-2401PC spectrometer. NMR spectra were recorded on a Bruker Avance III 600 MHz spectrometer, with TMS as an internal standard. ESIMS and HRESIMS were measured on an API QSTAR Pulsar 1 spectrometer. C-18 silica gel (40–60 μm; Daiso Co., Japan), MCI gel CHP 20P (75–150 μm, Mitsubishi Chemical Industries, Tokyo, Japan), Sephadex LH-20 (Amersham Pharmacia, Uppsala, Sweden), and silica gel (200-300 mesh; Qingdao Marine Chemical Inc., PR China) were used for column chromatography. silica gel GF254 (Qingdao Marine Chemical Inc., People’s Republic of China) was used for preparative TLC. Semi-preparative HPLC was carried out using an Agilent 1200 liquid chromatograph with a YMC-Pack ODS-A column (250 mm × 10 mm, i.d., 5 μm).
8
Spectroscopic Characterization of Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotations were obtained with a Jasco P-1020 Automatic Digital Polariscope. UV spectrum was measured with a Shi madzu UV2401PC spectrometer. IR spectra were obtained on a Bruker FT-IR Tensor-27 infrared spectrophotometer with KBr pellets. 1H, 13C, and 2D NMR spectra were recorded on a Bruker AM-400, Bruker DRX-500 NMR and Bruker DRX-600 spectrometer with TMS as internal standard. ESI-MS and HR-EI-MS analysis were carried out on Waters Xevo TQS and Waters AutoSpec Premier P776 mass spectrometers, respectively. Semi-preparative HPLC was performed on an Agilent 1100 HPLC with a ZORBAX SB-C18 (9.4 × 250 mm) column. Silica gel (100–200 and 200–300 mesh, Qingdao Marine Chemical Co. Ltd., P.R. China), Sephadex LH-20 (GE Healthcare Bio-Xciences AB), and MCI gel (75–150 μm, Mitsubishi Chemical Corporation, Tokyo, Japan) were used for column chromatography. Fractions were monitored by TLC (GF 254, Qingdao Marine Chemical Co. Ltd., P.R. China), and spots were visualized by 10 % H2SO4-ethanol reagent.
9
Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Opitical rotations were measured on an Autopol VI (Serial #91058) manufactured by Rudolph Research Analytical, Hackettstown, NJ, USA. UV spectra were recorded by a Shimadzu UV-2401PC spectrometer. CD spectra were recorded using a JASCO J-810 spectropolarimeter. NMR spectra were recorded on Bruker Avance III 600 spectrometers. Electrospray ionization (ESI)-high-resolution mass spectrometry (HRMS) were recorded on an Agilent G6230 TOF spectrometer. Single crystal X-ray crystallography was determined on SMART APEX II DUO X-ray single crystal diffractometer using Cu Kα radiation. Preparative HPLC was performed on a Waters 2489 series instrument with a UV/Visible detector, using a reversed-phase C18 column (Phenomenex, 250 mm × 21.2 mm, 5 μm). Chiral HPLC was carried out on an Agilent 1260 liquid chromatograph, utilizing chiral analytical columns [(R, R) WHELK-01 column, 4.6 mm × 250 mm, 10 μm, 100 A].
10
Characterizing SWCNT-based Transparent Conductive Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The
SWCNT-TCF transparency
was assessed by measuring the visible light transmittance (T %) at 550 nm in a Shimadzu UV-2401PC spectrometer. The
film thickness was calibrated with a Bruker Stylus Profiler model
Dektak XT.
The sheet resistance was measured in a two-probe
configuration using a Keithley 4200 unit provided with tungsten needles.
Two silver electrodes were painted along the longest sides of each
SWCNT-PET and SWNCT-PVDF film. Sheet resistance (Rs) values were calculated taking into account the film
geometry, given by the distance between the electrodes and their length.
The homogeneity of the films and the changes caused by the immersion
treatment were evaluated by Raman spectroscopy and microscopy techniques.
Raman spectra were acquired under ambient conditions using a LabRAM
HR Raman spectrometer (Horiba Jobin-Yvon) with a laser excitation
energy of 2.33 eV (532 nm). Raman maps of 400 data points were collected
with lateral steps of 1 μm, in both X and Y directions, on rectangular areas. Microscopic characterization
was conducted in a field emission scanning electron microscope (SEM)
model MERLIN (Carl Zeiss, Switzerland), whereas atomic force microscopy
(AFM) measurements were carried out in Multimode SPM equipment from
Veeco Instruments (Santa Barbara, U.S.).
SWCNT-TCF transparency
was assessed by measuring the visible light transmittance (T %) at 550 nm in a Shimadzu UV-2401PC spectrometer. The
film thickness was calibrated with a Bruker Stylus Profiler model
Dektak XT.
The sheet resistance was measured in a two-probe
configuration using a Keithley 4200 unit provided with tungsten needles.
Two silver electrodes were painted along the longest sides of each
SWCNT-PET and SWNCT-PVDF film. Sheet resistance (Rs) values were calculated taking into account the film
geometry, given by the distance between the electrodes and their length.
The homogeneity of the films and the changes caused by the immersion
treatment were evaluated by Raman spectroscopy and microscopy techniques.
Raman spectra were acquired under ambient conditions using a LabRAM
HR Raman spectrometer (Horiba Jobin-Yvon) with a laser excitation
energy of 2.33 eV (532 nm). Raman maps of 400 data points were collected
with lateral steps of 1 μm, in both X and Y directions, on rectangular areas. Microscopic characterization
was conducted in a field emission scanning electron microscope (SEM)
model MERLIN (Carl Zeiss, Switzerland), whereas atomic force microscopy
(AFM) measurements were carried out in Multimode SPM equipment from
Veeco Instruments (Santa Barbara, U.S.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!