Parafilm m

Manufactured by Amcor

Sourced in United States

Parafilm M is a self-sealing, flexible, thermoplastic film that can be used to temporarily seal and cover laboratory containers and equipment. It is designed to provide an air-tight and water-resistant barrier.

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Lab products found in correlation

Ct pro 3d, by Nikon (5 mentions) Xt h 225 st microfocus ct scanner, by Nikon (4 mentions) Stereo microscope model s6d, by Leica (2 mentions) Vgstudio max 2, by Volume Graphics (2 mentions) Multimetal target, by Nikon (2 mentions) Vgstudio max, by Volume Graphics (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Lsm 880, by Zeiss (2 mentions) Sca20, by Dataphysics (1 mentions) Hpm 010, by Oerlikon Balzers (1 mentions) Parafilm, by Amcor (1 mentions) Perfection v700 photo, by Epson (1 mentions) Analytical and hplc grade solvents, by Merck Group (1 mentions) Donepezil hcl, by Tokyo Chemical Industry (1 mentions) Prestoblue, by Thermo Fisher Scientific (1 mentions) Ms h pro, by Scilogex (1 mentions) Hcs cellmask deep red stain, by Thermo Fisher Scientific (1 mentions) Laminin 1, by Bio-Techne (1 mentions) Sorvall legend rt, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Parafilm (110 citations) Parafilm M® laboratory film (4 citations) Parafilm M film (2 citations)

62 protocols using parafilm m

Surface Tension and Contact Angle Measurement

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Static surface tension was determined using a Kruss K20S tensiometer. The equipment was calibrated with deionized water. After calibration, spray solutions were prepared and transferred to a receiving equipment placed in the tensiometer and were adjusted until the tensiometer sensor remained immersed in solution. The equipment platform gradually moved as the sensor separated from the solution surface. Surface tension was obtained in N m⁻¹ units after automatic calibration with deionized water.
Contact angle measurements were obtained with the Contact Angle System OCA 15-plus software (DataPhysics Instruments GmbH, Filderstadt, Germany) while automation and processing of computer images were conducted with the SCA20 software (DataPhysics Instruments GmbH, Filderstadt, Germany). Droplets were formed at the needle tip by an automatic triggering injector. Small volumes obtained through a precise syringe plunger movement produced 3 µL volume droplets which were deposited on a paraffin plastic film with a paper surface (Parafilm M, Bemis NA). Droplets were evaluated every second during a total of 60 s. Contact angle results were standardized at 10 s in all treatments for comparison purposes. Static surface tension and contact angle experimental design was complete random with four repetitions for each treatment.

Ferreira P.H., Thiesen L.V., Pelegrini G., Ramos M.F., Pinto M.M, & da Costa Ferreira M. (2020). Physicochemical properties, droplet size and volatility of dicamba with herbicides and adjuvants on tank-mixture. Scientific Reports, 10, 18833.

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Thiamethoxam Ingestion Assay for Diaphorina citri

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The ingestion assay was comprehensively described in Langdon and Rogers (2017) (link). Briefly, a 30% sucrose solution was used as the base artificial diet. Serial dilution was conducted to form eight doses of spiked diet using formulated Platinum 75SG (750 g thiamethoxam kg, Syngenta Crop Protection, Greensboro, NC). The caps of 5 ml snap-cap centrifuge tubes (Eppendorf Tubes, Hamburg, Germany, Cat. No.: 0030119401) were filled with 0.7 ml of each prepared dose. Parafilm M (Bemis, Neenah, WI, Cat. No.: PM-992) was stretched over each diet-filled cap. Four to six adult D. citri were loaded into each centrifuge tube and the diet-filled cap was reinstalled. Tubes were held upright in a tube tray at 27°C, 70% relative humidity, with a 14:10 (L:D) h photoperiod for 72 h. One replicate was defined as a single tube and 10 replicates were used for each dose. Between 40 and 60 adults were tested for each dose. After 72 h, insects were scored as alive (full function), moribund (insects lacking coordinated movement), or dead (no movement upon disturbing). Moribund insects were classified as dead for data analysis.

Langdon K.W., Schumann R., Stelinski L.L, & Rogers M.E. (2018). Influence of Tree Size and Application Rate on Expression of Thiamethoxam in Citrus and Its Efficacy Against Diaphorina citri (Hemiptera: Liviidae). Journal of Economic Entomology, 111(2), 770-779.

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Cryofixation and Freeze Substitution of RBL Cells

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RBL cells were maintained adherent on sapphire disks (3 mm in diameter, 0.05 mm in thickness) that were coated with poly-d-lysine and fibronectin. Each substrate was incubated with the disks overnight. The sapphire disks attached with cells were placed on Parafilm M (Bemis, Oshkosh, WI) until cryofixation, which was etched by needles. The disks were dipped in a cryoprotectant 1-hexadecane before being frozen in a high-pressure freezing machine (HPM 010; Balzers). After freezing, cells were dehydrated by freeze substitution in an acetone solution containing 1% osmium and 1% water as described (McDonald et al., 2010 (link)). The frozen samples were placed at −85°C for 72 h and then moved to −20°C. Samples were incubated for 24 h at −20°C and brought to− 4°C for 4 h. Finally, the samples were brought up to room temperature for 2 h and rinsed with dry acetone. Rinsed samples were embedded as follows: incubation in resin mixed of 25% Epon and 75% plastic for 1 h, 50% Epon and 50% acetone overnight, 75% Epon and 25% acetone for 1 h, and 100% Epon in vacuum for 4 h, and finally the 100% Epon was polymerized at 60°C for 48 h. The resin-containing cells were sectioned with ∼90-nm thickness and contrasted by aqueous 2% uranyl acetate for 5–20 min immediately before imaging under a Philips CM120 STEM system.

Woo S.S., James D.J, & Martin T.F. (2017). Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles. Molecular Biology of the Cell, 28(6), 792-808.

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Evaluating VOCs from P. oligandrum on N. benthamiana

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To investigate the effect of VOCs produced by P. oligandrum on seedling growth, five N. benthamiana seedlings of equal size were placed on one side of a bipartite petri plate containing Murashige and Skoog (MS) medium (0.8% agar, 1.5% sucrose [pH 5.7]), a 5-mm mycelial plug of actively growing P. oligandrum was placed on the other side of the petri plate containing V8 agar medium, and the plates without P. oligandrum were used as a control. The petri plates were sealed by wrapping them at least nine times with Parafilm (Parafilm M; Bemis, USA) to avoid the escape of VOCs and placed in an environmentally controlled chamber with 16-h-light and 8-h-dark cycles at 25°C. The seedling shoot length and dry weight were measured after 10 days. To measure the dry weight, plant material was dried in an oven until it reached a constant weight, which indicated that all moisture was removed. The experiment was repeated three times, each time with three replicates, and each replicate included five seedlings. Root morphological parameters were measured using a RhizoScan scanner (Epson Perfection V700 Photo; Epson, USA) and analyzed by WinRHIZO software (Regent Instruments Co., Canada) (78 (link)).

Sheikh T.M., Zhou D., Ali H., Hussain S., Wang N., Chen S., Zhao Y., Wen X., Wang X., Zhang J., Wang L., Deng S., Feng H., Raza W., Fu P., Peng H., Wei L, & Daly P. (2023). Volatile Organic Compounds Emitted by the Biocontrol Agent Pythium oligandrum Contribute to Ginger Plant Growth and Disease Resistance. Microbiology Spectrum, 11(4), e01510-23.

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Multifunctional Surface Coating Protocol

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Solutions of CuSO₄ (5 mM), THPTA (10 mM), MOI-N₃ (1
μM–0.5 mM), and p-DOPAmide
(10–50 mM) were prepared using one of the following buffers:
4-morpholineethanesulfonic acid (MES, pH 5.5), phosphate-buffered saline (PBS,
pH 7.4), or tris(hydroxymethyl)aminomethane (Tris, pH 8.5). These solutions were
combined in an Eppendorf vial to provide a master coating mixture (MCM). Unless
otherwise specified, all the buffers and reagent solutions were purged with
N₂ gas (for ~15 min). A specified volume of the MCM was
dropped onto a material in a tissue culture polystyrene plate (TCPS) or Petri
dish, which was then sealed with Parafilm M (Bemis) and gently agitated on a
shaker at 37 °C. After the coating is complete (typically within 30
min–4 h unless otherwise stated), the substrate was rinsed thoroughly
with Milli-Q water and dried under air and at room temperature (RT).
Planar solid materials used in this study include Ti/TiO₂,
Si/SiO₂, glass, polytetrafluoroethylene (PTFE), polyether ether
ketone (PEEK), polycarbonate (PC), silicone rubber (SiR), and a dime coin.
Two-dimensional porous or fibrous materials include a nylon foam and
polypropylene (PP) membrane. Three-dimensional objects include germanium pieces,
a plastic polyhedral dice, mini dinosaur toy, cherry tomato, lotus root, porous
Ti-based tissue scaffold, and Ti-based dental implant.

Jia Z., Hast K, & Izgu E.C. (2021). Catecholamine-Copper Redox as a Basis for Site-Specific Single-Step Functionalization of Material Surfaces. ACS applied materials & interfaces, 13(3), 4711-4722.

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Transwell Co-Culture Model for Macrophage-TCMK-1 Interaction

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The transwell co‐culture model was established based on the modification of a previously published method.²³ In the present model, TCMK‐1 cells and different phenotypes of macrophages were co‐cultured on the bottom and top surface of transwell microporous membrane, respectively. Briefly, a transwell insert with microporous membrane was gently wrapped around the edge using the sterilized parafilm (Parafilm M®, Bemis, USA) to build a parafilm fence. TCMK‐1 cells were then plated on bottom side of transwell insert membrane and were incubated for over 6 hours in medium at 37°C in 5% CO₂ to attach to the microporous membrane firmly. Next, the transwell insert was turned over and put back to allow TCMK‐1‐plated side to face down. Polarized macrophages were seeded onto the top side of membrane. Finally, the resultant transwell plate was incubated at 37°C in 5% CO₂ in ECM. Thus, TCMK‐1 cells formed the first layer on bottom side of the membrane, while macrophages formed the second layer on top side. The layers were isolated by the membrane, but the medium could diffuse freely across the membrane.

Juan C., Mao Y., Cao Q., Chen Y., Zhou L., Li S., Chen H., Chen J., Zhou G, & Jin R. (2021). Exosome‐mediated pyroptosis of miR‐93‐TXNIP‐NLRP3 leads to functional difference between M1 and M2 macrophages in sepsis‐induced acute kidney injury. Journal of Cellular and Molecular Medicine, 25(10), 4786-4799.

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Targeted miRNA Delivery via Microbubbles

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SVEC4-10 cells were seeded into 35 × 10 mm Petri dishes (Sarstedt, Neumbrecht, Germany) and incubated to allow attachment overnight. Petri dishes were carefully filled with PBS with calcium and magnesium to the brim and taped shut using Parafilm M (Bemis, Neenah, WI). The Targ^MB-M₁₂₆, Targ^MB-A₁₂₆, or Targ^MB-S₁₂₆ was injected using a 30 G needle into the Petri dishes through the Parafilm M. The Petri dishes were placed with the bottom facing up and were gently shaken for 5 min. A washing step was performed to remove unbound MBs before ultrasound bursting was employed to destroy the bound MBs and enable the miR to enter the cells. For washing, the Parafilm M was removed, the supernatant was discarded, and fresh media were added. Ultrasound was performed on the cells using color Doppler mode for 30 s per field of view (4 fields of view per Petri dish) to destroy the MBs. Cells were incubated for 48 hr before microscopy.

Wang X., Searle A.K., Hohmann J.D., Liu A.L., Abraham M.K., Palasubramaniam J., Lim B., Yao Y., Wallert M., Yu E., Chen Y.C, & Peter K. (2018). Dual-Targeted Theranostic Delivery of miRs Arrests Abdominal Aortic Aneurysm Development. Molecular Therapy, 26(4), 1056-1065.

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Exfoliation and Colloidal Processing of α-ZrP

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All used materials are listed with CAS numbers, supplier and purity in the supporting information. Synthesis and purification of α-ZrP, exfoliation processes with amines and colloidal processing as well as the preparation of samples with polymer colloids are described in detail in the supporting information. Syntheses were performed on decimolar scale while samples for analysis and rheological characterization were prepared in quantities of 10 to 100 ml. For mixing only blade and magnetic stirring was applied if at all. All samples were stored under ambient conditions and not shielded against artificial lighting. Cuvettes for long time observation were additionally sealed with parafilm (Parafilm M, Bemis, Neenah, WI, USA). Samples for film formation studies were drawn manually with a frame (150 μm slit) on Kapton® foil (Kapton 150FN999, DuPont, Midland, MI, USA) under ambient conditions. If not noted otherwise, all volume fractions are calculated by taking into account the amine loss during the centrifugation and the freeze-drying step as well as the amount of physisorbed water in the obtained powder.

Lin X., Schmelter D., Imanian S, & Hintze-Bruening H. (2019). Hierarchically Ordered α-Zirconium Phosphate Platelets in Aqueous Phase with Empty Liquid. Scientific Reports, 9, 16389.

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Shock Wave Exposure Protocol for Cells

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Cells were treated with rLLB waves generated by a modified miniature manual Reddy-tube device [50 (link)] as shown in Figure 1. Briefly, the device consists of a syringe, two adapters, a shim, two Parafilm M membranes, and a T25 flask. A 10 mL syringe acts as the driving section (the length of the driving section is 3.4 cm). Two Parafilm M (Bemis) membranes act as septa, which are compressed by adapter 1 and the shim. They were connected to the T25 cell culture flask by adapter 2. The end of adapter 2 acts as the driven section (the length of the driven section is 1 cm). When the cell density reached 80%, we removed the medium and filled the T25 cell culture flask with phosphate buffer (PBS). A miniature manual Reddy-tube device was connected to the cell culture flask, and the syringe was pushed by a manually operated piston to compress the air in the driving section to break through the septum, generating a shock wave to the cells in the flask. This was repeated 10 times to simulate rLLB exposure. The time between each shock wave treatment was about 2 min due to the experimental operation. Peak overpressure duration was less than 1 ms. For the NC group, the Reddy-tube device was installed using the same procedure but without the addition of Parafilm, and no shock wave was generated.

Geng C., Wang X., Chen J., Sun N., Wang Y., Li Z., Han L., Hou S., Fan H., Li N, & Gong Y. (2023). Repetitive Low-Level Blast Exposure via Akt/NF-κB Signaling Pathway Mediates the M1 Polarization of Mouse Alveolar Macrophage MH-S Cells. International Journal of Molecular Sciences, 24(13), 10596.

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Plant Grafting for Genetic Studies

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Wedge grafting was performed on the stems of 3-week-old G. max, 4-week-old N. benthamiana, and 5-week-old Arabidopsis plants, as described previously [6 (link)]. N. benthamiana and G. max stems were cut and trimmed to a V-shape. The inflorescence stems of Arabidopsis were cut and split from the stem middle at the graft site. The V-shaped stem was inserted into a middle-split stem and fixed using wrapping film (Bemis Parafilm M) or clip to form a graft union. The scions were covered with water-sprayed plastic bags and grown in an incubator at 27°C under continuous light (30 μmol m⁻² s⁻¹) for 10 d. Afterward, plastic bags were removed from the scions, the grafts were transferred to a 23°C growth room with 30% relative humidity, and 100 μmol m⁻² s⁻¹ continuous illumination. CMV-infected grafts were grown in a 23°C incubator for 10 days. The other experimental conditions were the same as those mentioned above. The scion outgrowth length was measured from the scion top node to the bottom node of the scion outgrow part (Figure 1, B and C) and recorded every week from 7 DAG (days after grafting) to the next several weeks.

Huang C., Kurotani K.I., Tabata R., Mitsuda N., Sugita R., Tanoi K, & Notaguchi M. (2023). Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting. Horticulture Research, 10(6), uhad072.

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