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2 protocols using desethylamiodarone hydrochloride

1

Amiodarone Metabolism Profiling

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Dulbecco’s Modified Eagle’s Medium (DMEM), amiodarone hydrochloride, formic acid, dimethyl sulfoxide (DMSO), α-naphthoflavone, and ketoconazole were purchased from Sigma-Aldrich (St. Louis, MO). Desethylamiodarone hydrochloride, amiodarone-d4 hydrochloride, and desethylamiodarone-d4 hydrochloride were obtained from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Blasticidin S hydrochloride was purchased from Life Technologies (Grand Island, NY). Human microsomes were obtained from BioreclamationIVT (Baltimore, MD). Water and acetonitrile were LC–MS grade and obtained from Fisher Scientific (Pittsburgh, PA).
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2

Amiodarone Hydrochloride Synthesis and Analysis

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Amiodarone hydrochloride and benzyldimethylphenyl ammonium chloride were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 2,5-Dihydroxybenzoic acid (DHB) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dilauroyl phosphatidylcholine was purchased from Wako Pure Chemical Co. Ltd. (Osaka, Japan). DesethylAmiodarone hydrochloride was purchased from Toronto Research Chemicals (Toronto, ONT, Canada). All other chemicals were available as analytical or high grade.
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