Dabrafenib

BMS-345541 was purchased from Sigma (Saint-Quentin-Fallavier, France). Vemurafenib, dabrafenib and trametinib were purchased from Euromedex (Souffelweyersheim, France) and TNFα was from PeproTech (Neuilly-Sur-Seine, France). Antibodies against CD271 was purchased from BD Biosciences (Franklin Lakes, NJ, USA), anti-ERK(D-2); anti-cleaved-PARP, anti-cleaved caspase-3, anti-pERK, anti-caspase-3, anti-JARID1B and TNFα blocking antibody were from Cell Signaling Technology Inc. (Beverly, MA, USA). Anti-IKK and anti-IκBα monoclonal antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-ABCB5 monoclonal antibody was purchased from Novus Biologicals (Abingdon, UK). Annexin V was purchased from Roche Diagnostic corporation (Indianapolis, IN, USA).

Trypan blue, Dulbecco's modified Eagle's medium, RPMI, trypsin, and siRNA were purchased from Life Technologies. Annexin V and DAPI were purchased from Roche Applied Science. Dabrafenib and PLX4032 were purchased from Euromedex. Antibodies against HSP90, E2F1, E2F4, Rb, and phospho-Rb were from Santa Cruz Biotechnology (TEBU, Le Perray en Yvelines, France). Antibodies against PARP, pro-caspase-3, p38, phospho-p38, MDM2, Chk2, p-Chk2, p21, and p27 were from BD Bioscience (Pont de Claix, France) and anti-H2AX was from Merck Millipore (Fontenay-sous-Bois, France).
The E2F1 siRNA number 1 corresponds to a Smart pool (Dharmacon ref: L-003259-00-0005) and the siRNA number 2 was purchassed to Ambion (Ref: 4390824).

6-(4-Chlorophenyl)imidazo [2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl) oxime (CITCO), clotrimazol, Pregnenolone 16 alpha-carbonitrile (PCN) and SR12813 were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Dabrafenib was purchased from Euromedex (Souffelweyersheim, France). SPA70 was purchased from Axon (Groningen, The Netherlands). Drugs were solubilized in DMSO at 10 mM and stored at −20 °C.