Cells were seeded in 6-well plates and treated with PAMD, 5-Fu or saline for 24 h. The cells were harvested, washed with phosphate-buffered saline (PBS), and fixed with 70% ethanol at −20°C overnight. After an additional washing, cells were incubated with RNase A (20 μg/mL) at 37°C for 30 min, stained with propidium iodide (100 μg/mL; Sigma-Aldrich) for 10 min, and analyzed with flow cytometry (BD FACSC autoTM II).
Facsc auto 2
Manufactured by BD
The BD FACSC autoTM II is a flow cytometer designed for automated cell analysis and sorting. It provides accurate and reproducible data by automating sample handling and flow cytometry processes. The core function of the BD FACSC autoTM II is to perform high-throughput cell analysis and sorting.
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Lab products found in correlation
5 protocols using facsc auto 2
1
Cell Cycle Analysis by Flow Cytometry
2
Apoptosis Evaluation of PAMD and 5-Fu
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Cells were seeded in 6-well plates and treated with PAMD, 5-Fu or saline for 24 h. Apoptosis was determined with the Annexin-V: FITC Apoptosis Detection Kit I (BD Biosciences) according to the manufacturer's protocol. Briefly, the saline control and drug-treated tumor cells were collected via centrifugation and washed once with PBS. The cells were subsequently stained with fluorescein and propidium iodide for 15 min at room temperature and analyzed with flow cytometry (BD FACSC autoTM II).
3
Cell Cycle Analysis by Flow Cytometry
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Cells were seeded in 6-well plates and treated with various doses of FW-04-806 or vehicle (DMSO) for 24 h. The cells were harvested, washed with phosphate-buffered saline (PBS), and fixed with 70% ethanol at −20°C overnight. After an additional washing, cells were incubated with RNase A (20 μg/mL) at 37°C for 30 min, stained with propidium iodide (100 μg/mL; Sigma Aldrich) for 10 min, and analyzed with flow cytometry (BD FACSC autoTM II).
4
Cell Cycle Analysis by Flow Cytometry
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Cells were seeded in 6-well plates and treated with various concentrations of FM807 for 24 h. Cells were harvested, washed with phosphate-buffered saline (PBS), and fixed in 70% ethanol overnight at −20°C. After additional washing, cells were incubated with RNase A (20 μg/mL) at 37°C for 30 min, stained with propidium iodide (100 μg/mL; Sigma Aldrich) for 10 min, and analyzed with flow cytometry (BD FACSC autoTM II).
5
Cell Cycle Analysis by Flow Cytometry
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Cells (4×10 5 cells/well) were seeded in 6-well plates and treated with various doses of SKF-96365, DES or 2-APB for 48 hours. The cells were harvested, and washed with phosphate-buffered saline (PBS), and then fixed with 70% ethanol at -20℃ overnight. After an additional washing, cells were incubated with RNase A (20 µg/mL) at 37℃ for 30 min, stained with propidium iodide (100 µg/mL; Sigma Aldrich) for 10 min, and analyzed with flow cytometry (BD FACSC auto TM II).
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