Caffeine (99%), CGAs (99%), trigonelline (99%), and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Titan Technology Co., Ltd. (Shanghai, China) provided 2-octanol and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS). Sigma–Aldrich Chemical Co., Ltd. (Shanghai, China) provided Folin-phenol (2M).
Folin phenol
Manufactured by Merck Group
Sourced in China
Folin-phenol is a reagent used in colorimetric assays to determine the total protein content of a sample. It reacts with the amino acid residues in proteins, producing a blue-colored complex that can be measured spectrophotometrically. The intensity of the color is proportional to the protein concentration in the sample.
Automatically generated - may contain errors
Lab products found in correlation
1 1 diphenyl 2 picrylhydrazyl radical, by Yuanye Bio-Technology (1 mentions)
Caffeine, by Yuanye Bio-Technology (1 mentions)
Trigonelline, by Yuanye Bio-Technology (1 mentions)
Chlorophyll a, by Merck Group (1 mentions)
Ninhydrin, by Shanghai Chemical Reagent (1 mentions)
Chlorophyll b, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Zinc acetate, by Merck Group (1 mentions)
Potassium ferrocyanide, by Merck Group (1 mentions)
Galacturonic acid, by Merck Group (1 mentions)
Sulfuric acid, by Sinopharm (1 mentions)
Sodium potassium tartrate, by Merck Group (1 mentions)
Anhydrous ethanol, by Sinopharm (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
N hexane, by Merck Group (1 mentions)
Phenol, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
4 protocols using folin phenol
1
Antioxidant Compound Characterization
2
Quantitative Analysis of Pigments
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The main chemicals used in this study included ninhydrin, anthrone, concentrated sulfuric acid, and acetone (AR, China national pharmaceutical group, Shanghai chemical reagent Co., Ltd.); Folinphenol (AR, Sigma, USA); methanol and ether (AR, Fisher Scientific, Ireland); chlorophyll a, and chlorophyll b (standard products, Sigma-Aldrich, USA); lutein and β-carotene (standard products, Shanghai Yuanye Biotechnology Co., Ltd.).
3
Comprehensive Analytical Protocols for Fruit and Vegetable Characterization
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All solvents and chemicals listed below were analytical grade and purchased from Sinopharm Chemical Reagent Beijing Co., Ltd (Beijing, China): anhydrous ethanol (C2H5OH), sulfuric acid (H2SO4), carbazole (C12H9N), sodium hydroxide (NaOH), ɑ‐naphthol (C10H8O), oxalic acid (C2H2O4), sodium bicarbonate (NaHCO3), sodium chloride (NaCl), n‐hexane (CH3(CH2)4CH3), acetone (C3H6O), BHT, folin‐phenol, sodium carbonate (Na2CO3), methanol (CH3OH), sodium nitrite (NaNO2), aluminum hydrate nitrate (Al(NO3)3.9H2O), fructose (C6H12O6), sucrose (C12H22O11), hydrochloric acid (HCl), phenol (C6H5OH), methyl red (C15H15N3O2), zinc acetate (Zn(CH3COO)2), potassium ferrocyanide (K4Fe(CN)6.3H2O), potassium sodium tartrate (C4H4O6KNa·4H2O), 3,5‐dinitrosalicylic acid (C7H4N2O7).
Standards of galacturonic acid, rutin, gallic acid, L(＋)‐ascorbic acid, glucose, sucrose, and fructose (purity﹥99%) were of HPLC grade and obtained from the Sigma‐Aldrich (St. Louis, MO, USA.).
Standards of galacturonic acid, rutin, gallic acid, L(＋)‐ascorbic acid, glucose, sucrose, and fructose (purity﹥99%) were of HPLC grade and obtained from the Sigma‐Aldrich (St. Louis, MO, USA.).
4
Bacillus Strain Isolation and Characterization
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Defatted SBM was purchased from Shandong Koufu Oils & Grains Co., Ltd. (Binzhou, China). Folin–phenol, phytic acid, sulfosalicylic acid, cetyltrimethylammonium bromide, sodium dodecyl sulfate, amylase, and ethylenediaminetetraacetic acid were purchased from Sigma–Aldrich (St. Louis, MO, United States). All other chemicals were of analytical grade locally.
Bacillus sp. strains named: B-1, B-2, B-3, B-4, B-5, B-6, B-7, B-8, B-9, B-10, B-11, G4, M1, M-2, M3, M4, M10, 46, W-18, Lipro-1, Lipro-2, J-4, DB-7-6, ED-3-7, Y4, N-2, 2-27, N-11, 20, and 57 were isolated and stored in our laboratory. All strains were microscopically examined using Gram- and Schaeffer–Fulton stains to confirm that they were Bacillus spp.
Bacillus sp. strains named: B-1, B-2, B-3, B-4, B-5, B-6, B-7, B-8, B-9, B-10, B-11, G4, M1, M-2, M3, M4, M10, 46, W-18, Lipro-1, Lipro-2, J-4, DB-7-6, ED-3-7, Y4, N-2, 2-27, N-11, 20, and 57 were isolated and stored in our laboratory. All strains were microscopically examined using Gram- and Schaeffer–Fulton stains to confirm that they were Bacillus spp.
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