Mx1 cre, by Jackson ImmunoResearch - Product details

1

Genetic Manipulation of Dpy30 and P21 in Mice

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All animal procedures were approved by the Institutional Animal Care and Use Committee at the University of Alabama at Birmingham. All mice were maintained under specific pathogen-free conditions and housed in individually ventilated cages. Dpy30^+/− mice were generated in our laboratory as reported previously (Yang et al., 2016 (link)) and were crossed to Mx1-Cre (Jackson Laboratory, JAX 003556) and Vav-Cre (Jackson Laboratory, JAX 008610) mice to produce Cre; Dpy30^+/− Mice. These mice were further crossed with Dpy30^F/F mice to produce Cre; Dpy30^F/+ and Cre; Dpy30^F/− littermates for experimental use. All transplant recipient mice were C57Bl/6J and CD45.1⁺, and purchased from Charles River Laboratories. P21^−/− (129S2-Cdkn1atm1Tyj/J) mice (Jackson Laboratory, JAX 003263) were further crossed with Mx1-Cre and Dpy30^F/F to generate the littermates of Dpy30^F/F; P21^+/−; Mx1-Cre and Dpy30^F/F; P21^−/−; Mx1-Cre.

Yang Z., Shah K., Khodadadi-Jamayran A, & Jiang H. (2019). Control of Hematopoietic Stem and Progenitor Cell Function through Epigenetic Regulation of Energy Metabolism and Genome Integrity. Stem Cell Reports, 13(1), 61-75.

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2

Transgenic Mouse Models of E2A-PBX1 Leukemia

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Conditional E2A-PBX1 transgenic mice were reported previously (5 (link)). Transgenic CD19.Cre (Jackson laboratory, (6 (link))), Mb1.Cre (provided by Dr. David Allman, University of Pennsylvania, Philadelphia, PA, USA; and by Dr. Michael Reth, University of Freiburg, Germany (7 (link))) and Mx1.Cre (The Jackson Laboratory (8 (link))) mice were intercrossed to generate E2A-PBX1/CD19.Cre, E2A-PBX1/Mb1.Cre and E2A-PBX1/Mx1.Cre mice respectively, on a C57BL/6 background. Leukemia cells derived from E2A-PBX1/CD19.Cre and E2A-PBX1/Mx1.Cre mice, which were preBCR+ as seen by cytoplasmic μ chain, were used for in vitro and in vivo experiments. Leukemia cells derived from E2A-PBX1/Mb1.Cre mice, which were preBCR-, were used for in vitro experiments. Disease-free survival was defined when showing signs of illness including general lymphadenopathy, lethargy, weight loss and shivering. Moribund mice were euthanized.

Duque-Afonso J., Lin C.H., Han K., Wei M.C., Feng J., Kurzer J., Schneidawind C., Wong S.H., Bassik M.C, & Cleary M.L. (2016). E2A-PBX1 remodels oncogenic signaling networks in B-cell precursor acute lymphoid leukemia. Cancer research, 76(23), 6937-6949.

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3

Conditional Deletion of TRAF6 and IKKβ in Mice

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All mice were bred, housed, and handled in the Association for Assessment and Accreditation of Laboratory Animal Care-accredited animal facility of Cincinnati Children’s Hospital Medical Center (CCHMC) or Memorial Sloan Kettering Cancer Center (MSKCC). Animal care was in strict compliance with the institutional guidelines established by CCHMC and MSKCC, the Guide for the Care and Use of Animals, and the Association for Assessment and Accreditation of Laboratory Animal Care International. TRAF6^fl/fl mice (C57BL/6) were a kind gift from Dr. Yongwon Choi (University of Pennsylvania) (Han et al., 2013 (link)). Traf6^fl/fl mice were crossed with Mx1Cre (Jackson Laboratory, 003556) and VavCre mice (Jackson Laboratory, 008610) for inducible or conditional deletion of TRAF6 (Traf6^fl/fl;Mx1Cre) and (Traf6^fl/fl; VavCre), respectively. To delete TRAF6, Cre transgene expression in Traf6^fl/fl;Mx1Cre mice was induced by pIpC. IKKβ^fl/fl mice were previously described and a kind gift from Dr. Albert Baldwin (University of North Carolina) (Mankan et al., 2011 (link)). To delete IKKβ in hematopoietic cells, IKKβ^fl/fl mice were crossed with VavCre mice. BM cells were obtained by crushing the femur, tibia, and pelvic bone, and maintained in RPMI1640 with 10% fetal bovine serum.

Fang J., Muto T., Kleppe M., Bolanos L.C., Hueneman K.M., Walker C.S., Sampson L., Wellendorf A.M., Chetal K., Choi K., Salomonis N., Choi Y., Zheng Y., Cancelas J.A., Levine R.L, & Starczynowski D.T. (2018). TRAF6 Mediates Basal Activation of NF-κB Necessary for Hematopoietic Stem Cell Homeostasis. Cell reports, 22(5), 1250-1262.

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Conditional Knockout Mouse Models

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Mice were maintained in a pure C57BL/6 background and were kept under specific-pathogen-free conditions in the transgenic mouse facility of Baylor College of Medicine (BCM) (22–24 °C, 30%−70% humidity with 12h dark/12h light cycle). Sel1l^flox/flox, Ire1α^flox/flox, Xbp1^flox/flox and Mpl^−/− mice were described previously^{28 (link)–31}. The floxed mice were crossed with either Mx1-Cre (The Jackson Laboratory, 005673) or Vav-iCre (The Jackson Laboratory, 008610) mice to generate Sel1l^flox/flox:Mx1-Cre, Sel1l^flox/flox:Vav-iCre, Ire1α^flox/flox:Vav-iCre, or Xbp1^flox/flox:Vav-iCre mice. The Sel1l^flox/flox:Ire1α^flox/flox:Vav-iCre and Sel1l^flox/flox:Xbp1^flox/flox:Vav-iCre mice were generated by crossing Sel1l^flox/flox:Vav-iCre mice with Ire1α^flox/flox or Xbp1^flox/flox mice respectively. 8-week-old Sel1l^flox/flox:Mx1-Cre mice were intraperitoneally injected with poly(I:C) (GE) every other day (1 μg/g body weight) for three times. poly(I:C)-injected Sel1l^flox/flox littermates were used as controls. Both male and female mice were used. For transplantation assays, female eight-to-twelve-week-old C57BL/6-Ly5.1 (CD45.1⁺) mice (Charles river, 564) were used. All procedures were approved by the BCM Institutional Animal Care and Use Committee. The study is compliant with all relevant ethical regulations regarding animal research.

Xu L., Liu X., Peng F., Zhang W., Zheng L., Ding Y., Gu T., Lv K., Wang J., Ortinau L., Hu T., Shi X., Shi G., Shang G., Sun S., Iwawaki T., Ji Y., Li W., Rosen J.M., Zhang X.H., Park D., Adoro S., Catic A., Tong W., Qi L., Nakada D, & Chen X. (2020). Protein Quality Control Through Endoplasmic Reticulum-Associated Degradation Maintains Hematopoietic Stem Cell Identity and Niche Interactions. Nature cell biology, 22(10), 1162-1169.

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5

Conditional Knockout Mouse Models

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Mice were maintained in a pure C57BL/6 background and were kept under specific-pathogen-free conditions in the transgenic mouse facility of Baylor College of Medicine (BCM) (22–24 °C, 30%−70% humidity with 12h dark/12h light cycle). Sel1l^flox/flox, Ire1α^flox/flox, Xbp1^flox/flox and Mpl^−/− mice were described previously^{28 (link)–31}. The floxed mice were crossed with either Mx1-Cre (The Jackson Laboratory, 005673) or Vav-iCre (The Jackson Laboratory, 008610) mice to generate Sel1l^flox/flox:Mx1-Cre, Sel1l^flox/flox:Vav-iCre, Ire1α^flox/flox:Vav-iCre, or Xbp1^flox/flox:Vav-iCre mice. The Sel1l^flox/flox:Ire1α^flox/flox:Vav-iCre and Sel1l^flox/flox:Xbp1^flox/flox:Vav-iCre mice were generated by crossing Sel1l^flox/flox:Vav-iCre mice with Ire1α^flox/flox or Xbp1^flox/flox mice respectively. 8-week-old Sel1l^flox/flox:Mx1-Cre mice were intraperitoneally injected with poly(I:C) (GE) every other day (1 μg/g body weight) for three times. poly(I:C)-injected Sel1l^flox/flox littermates were used as controls. Both male and female mice were used. For transplantation assays, female eight-to-twelve-week-old C57BL/6-Ly5.1 (CD45.1⁺) mice (Charles river, 564) were used. All procedures were approved by the BCM Institutional Animal Care and Use Committee. The study is compliant with all relevant ethical regulations regarding animal research.

Xu L., Liu X., Peng F., Zhang W., Zheng L., Ding Y., Gu T., Lv K., Wang J., Ortinau L., Hu T., Shi X., Shi G., Shang G., Sun S., Iwawaki T., Ji Y., Li W., Rosen J.M., Zhang X.H., Park D., Adoro S., Catic A., Tong W., Qi L., Nakada D, & Chen X. (2020). Protein Quality Control Through Endoplasmic Reticulum-Associated Degradation Maintains Hematopoietic Stem Cell Identity and Niche Interactions. Nature cell biology, 22(10), 1162-1169.

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6

Conditional Deletion of TRAF6 and IKKβ in Mice

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All mice were bred, housed, and handled in the Association for Assessment and Accreditation of Laboratory Animal Care-accredited animal facility of Cincinnati Children’s Hospital Medical Center (CCHMC) or Memorial Sloan Kettering Cancer Center (MSKCC). Animal care was in strict compliance with the institutional guidelines established by CCHMC and MSKCC, the Guide for the Care and Use of Animals, and the Association for Assessment and Accreditation of Laboratory Animal Care International. TRAF6^fl/fl mice (C57BL/6) were a kind gift from Dr. Yongwon Choi (University of Pennsylvania) (Han et al., 2013 (link)). Traf6^fl/fl mice were crossed with Mx1Cre (Jackson Laboratory, 003556) and VavCre mice (Jackson Laboratory, 008610) for inducible or conditional deletion of TRAF6 (Traf6^fl/fl;Mx1Cre) and (Traf6^fl/fl; VavCre), respectively. To delete TRAF6, Cre transgene expression in Traf6^fl/fl;Mx1Cre mice was induced by pIpC. IKKβ^fl/fl mice were previously described and a kind gift from Dr. Albert Baldwin (University of North Carolina) (Mankan et al., 2011 (link)). To delete IKKβ in hematopoietic cells, IKKβ^fl/fl mice were crossed with VavCre mice. BM cells were obtained by crushing the femur, tibia, and pelvic bone, and maintained in RPMI1640 with 10% fetal bovine serum.

Fang J., Muto T., Kleppe M., Bolanos L.C., Hueneman K.M., Walker C.S., Sampson L., Wellendorf A.M., Chetal K., Choi K., Salomonis N., Choi Y., Zheng Y., Cancelas J.A., Levine R.L, & Starczynowski D.T. (2018). TRAF6 Mediates Basal Activation of NF-κB Necessary for Hematopoietic Stem Cell Homeostasis. Cell reports, 22(5), 1250-1262.

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7

Genetically Modified Mouse Models

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All animal experiments in this study were performed under an animal experimental protocol approved by the UMKC IACUC. Sh3bp2^KI/KI mice were reported previously.^{(19 (link))} c-Fos-deficient (c-Fos^−/−, #002293), Rankl^fl/fl (#018978), EIIa-Cre (#003724), Mx1-Cre (#003556), and Csf1r^fl/fl (#021212) mice were obtained from the Jackson laboratory. RANKL-deficient (Rankl^−/−) mice were created by crossing Rankl^fl/fl mice with EIIa-Cre mice. All mice were crossed and created on the mix background of C57BL/6 and 129X1/SvJ under specific pathogen free conditions. Csf1r^fl/fl mice with Mx1-Cre were injected with poly(I:C) (250 mg/body, GE Healthcare) intraperitoneally three times with 2-day intervals at 1 week old to induce Cre expression. Csf1r^fl/fl mice without Mx1-Cre were also injected with poly(I:C) as controls. There were no significant gender differences in phenotypes, thus samples from both males and females were pooled for statistical analysis.

Kittaka M., Mayahara K., Mukai T., Yoshimoto T., Yoshitaka T., Gorski J.P, & Ueki Y. (2017). Cherubism mice also deficient in c-Fos exhibit inflammatory bone destruction executed by macrophages that express MMP14 despite the absence of TRAP+ osteoclasts. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(1), 167-181.

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8

Mitochondrial Function in Mouse Development

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C57BL/6 Uqcrfs1 (RISP^{fl/fl)48 (link)} and C57BL/6 Tfam^{fl/fl,48 (link)} mice were described previously. Vav-iCre (Stock #008610) and Mx-1 Cre (Stock #003556) mice were obtained from the Jackson laboratory. Recipients in transplantation assays were adult C57BL/6 CD45.1 mice (Jackson Laboratory, stock#002014). L-buthionine-sulfoximine (Sigma) at 2 or 20 mM and N-acetylcysteine (Sigma) at 1 mg/mL were administered in drinking water to 8.5 d.p.c (days post coitum) pregnant females until the day fetuses were extracted. Poly I:C (GE) was injected intraperitoneally every other day for five days at a dose of 12mg/kg. All animal procedures were approved by Institutional Animal Care and Use Committee (IACUC) at Northwestern University.

Ansó E., Weinberg S.E., Diebold L.P., Thompson B.J., Malinge S., Schumacker P.T., Liu X., Zhang Y., Shao Z., Steadman M., Marsh K.M., Xu J., Crispino J.D, & Chandel N.S. (2017). The mitochondrial respiratory chain is essential for haematopoietic stem cell function. Nature cell biology, 19(6), 614-625.

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9

Conditional Mouse Models for Hematologic Malignancies

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All animals were housed at MSKCC and at Foundation for Biomedical Research and Innovation (FBRI, Japan) using a 12-hour light/12-hour dark cycle and with ambient temperature maintained at 72°F ± 2°F (∼21.5°C ± 1°C) with 30% to 70% humidity. All animal procedures were completed in accordance with the Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committees at MSKCC and FBRI. All mouse experiments were performed in accordance with a protocol approved by the MSKCC (11-12-029) and FBRI (18-06) Institutional Animal Care and Use Committee. Mx1-Cre and Sf3b1^K700E mice were obtained from The Jackson Laboratory and were previously generated, respectively.¹⁴ (link) inv(3)(3q21q26) mouse strain (RBRC09508) was provided by RIKEN BRC through the National BioResource Project of the MEXT/AMED, Japan.⁷ (link) All of the primers and polymerase chain reaction (PCR) conditions are listed in supplemental Table 1.

Tanaka A., Nakano T.A., Nomura M., Yamazaki H., Bewersdorf J.P., Mulet-Lazaro R., Hogg S., Liu B., Penson A., Yokoyama A., Zang W., Havermans M., Koizumi M., Hayashi Y., Cho H., Kanai A., Lee S.C., Xiao M., Koike Y., Zhang Y., Fukumoto M., Aoyama Y., Konuma T., Kunimoto H., Inaba T., Nakajima H., Honda H., Kawamoto H., Delwel R., Abdel-Wahab O, & Inoue D. (2022). Aberrant EVI1 splicing contributes to EVI1-rearranged leukemia. Blood, 140(8), 875-888.

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10

Genetic Mouse Models for Autophagy Research

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All animal procedures were approved by the Columbia University Institutional Animal Care and Use Committee, and are in accordance with the “Guide for the Care and Use of Laboratory Animals” by the National Institutes of Health. Mice were maintained on a 12h dark/light cycle with free access to food and water unless otherwise indicated. For all experiments, 8–10 week old mice were used unless otherwise indicated. Mx1-Cre, Albumin-Cre, Vav1-Cre and EIIa-Cre mice were obtained from Jackson. MHC-Cre mice have been described (Agah et al., 1997 (link)). GFP-LC3 mice (Mizushima et al., 2004 (link)) were obtained from RIKEN, Japan. Floxed Atg7 mice (Komatsu et al., 2005 (link)) and Alb-CreERT² mice (Schuler et al., 2004 (link)) have been described. Alb-CreERT² activity was induced by i.p. injection of 0.1 mg tamoxifen for five consecutive days.

Huebener P., Gwak G.Y., Pradere J.P., Quinzii C.M., Friedman R., Lin C.S., Trent C.M., Mederacke I., Zhao E., Dapito D.H., Lin Y., Goldberg I.J., Czaja M.J, & Schwabe R.F. (2014). High Mobility Group Box 1 is Dispensable for Autophagy, Mitochondrial Quality Control and Organ Function in Vivo. Cell metabolism, 19(3), 539-547.

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Mx1 cre

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39 protocols using mx1 cre

Genetic Manipulation of Dpy30 and P21 in Mice

Transgenic Mouse Models of E2A-PBX1 Leukemia

Conditional Deletion of TRAF6 and IKKβ in Mice

Conditional Knockout Mouse Models

Conditional Knockout Mouse Models

Conditional Deletion of TRAF6 and IKKβ in Mice

Genetically Modified Mouse Models

Mitochondrial Function in Mouse Development

Conditional Mouse Models for Hematologic Malignancies

Genetic Mouse Models for Autophagy Research

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