Sodium phenylbutyrate 4 pba

Mouse monoclonal antibodies against GAPDH and actin, rabbit polyclonal antibodies against PTEN, PERK, phosphorylated elF2 and CHOP were from Cell Signaling Technologies. β-tubulin III antibody, thapsigargin (TP) and sodium phenylbutyrate (4-PBA) were from Sigma-Aldrich. Anti-Phosphorylated PERK antibody was obtained from Santa Cruz Biotechnology. Aβ₁₋₄₂ was purchased from China Peptide. The mimic or inhibitors of miR-200 family and siPTEN/control siRNAs were synthesized by Life Technologies with sequences shown in Table 1.

Human PDAC cell lines CFPAC-1, SW1990, and BxPC3 were purchased from the Committee of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), and L3.7–2 and MIA-PaCa2 were obtained from the American Type Culture Collection (ATCC). SW1990, BxPC3, and L3.7–2 cells were cultured in Roswell Park Memorial Institute Medium (RPMI) 1640 containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S). MIA-PaCa2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS and 1% P/S. All cells were incubated at 37 °C and 5% CO₂ in humidified air.
The reagents used in the experiments were as follow: Ro5-3335 (#4694, TOCRIS), gemcitabine (#1,288,463, Sigma-Aldrich, St. Louis, MO), sodium phenylbutyrate (4-PBA, #1716–12-7, Sigma-Aldrich, St. Louis, MO), thapsigargin (S7895, Selleck) and GSK2606414 (#1,337,531–36-8, MedChemExpress).