pH (SIST ISO 10523:2008; pH/Conductivity meter SevenMulti, Mettler Toledo), Eh (SIST DIN 38404-6:2000; T 90-MB, Mettler Toledo), EC (SIST EN 27888:1998; pH/Conductivity meter SevenMulti, Mettler Toledo) and Cr(VI) concentrations (SIST ISO 11083:1996; Cary 1E (UV VIS), Varian) were determined in all of the batch leachate and pH-dependent leachate samples. The metals were analysed by either ICP-MS (SIST EN ISO 17294-2, 2005; Agilent 7500ce, Agilent Technologies Inc.) or ICP-OES (ISO 11885:2007, ICP-OES OPTIMA 2000 DV, Perkin Elmer). Additionally, the anion content (Cl−, F−, SO42−, NO3−) (ISO 10304-1:2007; IC, 761 COMPACT IC, Metrohm) and DOC (SIST ISO 8245:2000; Multi N/C 2100 S (CHD), Analytik Jena)) were determined.
Sevenmulti
Manufactured by Mettler Toledo
Sourced in Switzerland, United States, Poland, China, France
The SevenMulti is a versatile multi-parameter meter from Mettler Toledo. It measures various parameters such as pH, conductivity, dissolved oxygen, and ion concentration. The SevenMulti is designed for use in laboratories and can handle a wide range of applications.
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Lab products found in correlation
Densito 30px, by Mettler Toledo (3 mentions)
Zetasizer nano z, by Malvern Panalytical (2 mentions)
Inlab routine pro, by Mettler Toledo (2 mentions)
Dimethylsulfoxid, by Merck Group (1 mentions)
Potato dextrose agar (pda), by Avantor (1 mentions)
Mueller hinton broth (mhb), by Avantor (1 mentions)
U 2001 spectrophotometer, by Hitachi (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Up50h, by Hielscher (1 mentions)
Minolta chromameter cr 310, by Konica Minolta (1 mentions)
Hp 8453, by Agilent Technologies (1 mentions)
Cary 60 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Precisely clarus500, by PerkinElmer (1 mentions)
Jsm 6701f field emission scanning electron microscope, by JEOL (1 mentions)
Autosorb iq2, by Anton Paar (1 mentions)
Inlab expert pro ism, by Mettler Toledo (1 mentions)
Bid2010 plus, by Shimadzu (1 mentions)
4 methylvaleric acid, by Merck Group (1 mentions)
Trace tr wax, by Thermo Fisher Scientific (1 mentions)
49 protocols using sevenmulti
1
Comprehensive Characterization of Batch Leachates
2
Feline Urinary pH Measurement and Analysis
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The urinary pH of the cats was measured at 07.00 hours (fasting pH) and 12.00 hours (postprandial pH). The measurements were performed with an electronic pH meter (Seven Multi, Mettler-Toledo GmbH, Schwerzenbach, Switzerland).
For the analysis of urine composition, the urine was defrosted at room temperature. After measuring the total urine volume of each cat, a sample of 12 ml was taken for further analysis and the remainder frozen at -80°C. The pH of the sample was adjusted to a pH of 2 using hydrochloric acid (37%) (pH-meter Seven Multi, Mettler-Toledo). The added amount of hydrochloric acid was documented. The urine was filtered (Syringe Filter, Bulk, SFCA (surfactant-free cellulose acetate), 0,2μm, 25mm non-sterile, Thermo Scientific, Rochester, NY, USA) prior to the further analysis [23 (link)].
For the analysis of urine composition, the urine was defrosted at room temperature. After measuring the total urine volume of each cat, a sample of 12 ml was taken for further analysis and the remainder frozen at -80°C. The pH of the sample was adjusted to a pH of 2 using hydrochloric acid (37%) (pH-meter Seven Multi, Mettler-Toledo). The added amount of hydrochloric acid was documented. The urine was filtered (Syringe Filter, Bulk, SFCA (surfactant-free cellulose acetate), 0,2μm, 25mm non-sterile, Thermo Scientific, Rochester, NY, USA) prior to the further analysis [23 (link)].
3
Antimicrobial Potential of Natural Compounds
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Curcumin, dimethylsulfoxid (DMSO), lysogeny broth (LB) agar, phenalenone, and RPMI1640 medium were received from Merck KGaA (Darmstadt, Germany). Potato dextrose agar (PDA) and Mueller–Hinton broth (MHB) were purchased from VWR International (Vienna, Austria). Rose Bengal (RB) was received from TCI Europe (Zwijndrecht, Belgium). Hypericum perforatum extract (ethanol) was prepared from the pharmaceutical drug “Johanniskraut 600 mg forte” (Apomedica, Graz, Austria) by dissolving the filling of the film-coated tablet in ethanol after mechanical removal of the lactose. The 96-well plates (flat bottom) were bought from SARSTEDT (Nümbrecht, Germany).
The U-2001 spectrophotometer for adjusting the McFarland standard was from Hitachi (Chiyoda, Japan). For measurement of the 96-well plates, a Tecan Sunrise Remote Plate Reader (Tecan, Männedorf, Switzerland) was used. The adjustment of pH values was carried out with the pH-meter Mettler Toledo SevenMulti (Mettler-Toledo GmbH, Vienna, Austria).
The U-2001 spectrophotometer for adjusting the McFarland standard was from Hitachi (Chiyoda, Japan). For measurement of the 96-well plates, a Tecan Sunrise Remote Plate Reader (Tecan, Männedorf, Switzerland) was used. The adjustment of pH values was carried out with the pH-meter Mettler Toledo SevenMulti (Mettler-Toledo GmbH, Vienna, Austria).
4
Macroalgal Extract Production Protocol
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Macroalgal extract was produced according to the methodology described by Michalak et al. [49 (link)]. Dry C. glomerata biomass (4 g) was suspended in an aqueous solution (80 mL). The mixture was subjected to the ultrasound homogenizer for 30 min working at parameters: 50 W, ultrasonic frequency 30 kHz, amplitude 100% (UP 50H, Hielscher Ultrasonics, Teltow, Germany). Then, the mixture was centrifuged for 10 min at a speed rate of 4000 rpm. The obtained supernatant was treated as 100% (E100) macroalgal extract. The pH of the extract was measured in 4 replications with a pH meter (Mettler Toledo SevenMulti (Mettler-Toledo, Warsaw, Poland)). This concentration was used to prepare 20% (E20), 40% (E40), 60% (E60), and 80% (E80) extracts. Post-extraction residue was at first air-dried, then, milled using a grinding mill, and collected for further use as a biosorbent.
5
Egg Yolk and Albumen pH and Color
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The pH of egg yolk and albumen were measured by using a digital pH meter Mettler Toledo SevenMulti (Mettler-Toledo GmbH, Greifensee, Switzerland). For measurement of yolk color according to CIE (1986) L* a* b* color system a Minolta Chroma Meter CR-310 (Minolta Camera Co. Ltd, Osaka, Japan) was used. The L* value indicates the lightness, representing dark to light (0-100). The a* (redness) value indicates the degree of the redgreen color, with a higher positive a* value indicating more red color. The b* (yellowness) value indicates the degree of the yellow-blue color, with a higher positive b* value indicating more yellow color. Subjective yolk color was determined using the Roche Yolk Color Fan (DSM Nutritional Products, Kaiseraugst, Switzerland) by one person.
6
Homogenization and pH Measurement
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Samples (10 g) were homogenized in a blender with 90 mL of distilled water. The mixture was filtered through a filter paper (150, Hangzhou Special Paper Industry Co., Ltd., Fuyang China) and was then measured with a pH meter (SevenMulti, Mettler-Toledo, Schwerzenbach, Switzerland). The average of the triplicate measurements was obtained.
7
Hydrolytic Stability and pKa Determination
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Physicochemical studies: hydrolytic stability and pKa determination:
UV spectra were recorded with HP8453 (Agilent) temperature controlled spectrophotometer.
The pHs of the solutions were measured at room temperature (temperature probe) with a combined pH electrode with Seven Multi (Mettler Toledo) pHmeter.
UV spectra were recorded with HP8453 (Agilent) temperature controlled spectrophotometer.
The pHs of the solutions were measured at room temperature (temperature probe) with a combined pH electrode with Seven Multi (Mettler Toledo) pHmeter.
8
pH Stability Assessment of MBGs and Ag-MBGs
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The assessment of pH stability of MBGs and Ag-MBGs was carried out in a simulated body fluids (SBF) according to Yan et al. 25) with some modifications. Briefly, SBF was prepared according to Kokubo 26) . Then, 100 mg of MBGs or Ag-MBGs powders was immersed in 100 mL of SBF solution contained in a polyethylene bottle covered with a tight lid. The bottles were placed at temperature of 37ºC. The pH value of the SBF over time was tested by a pH meter (SevenMulti, Mettler-Toledo, Switzerland).
9
Water Quality Monitoring in Aquaculture
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During the experimental period, water samples were taken regularly from three sampling points using a pipette for analysis of different parameters. Three sampling points were set up in the system. Sampling point 1 was set in the two breeding tanks. The position of sampling point 2 was in the outlet of the combined electrolysis unit. Another sampling point was in the buffer pool. Sampling interval was 30 min. Total ammonia nitrogen (TAN), NO3−-N and NO2−-N concentrations were analyzed using a Cary 60 UV-vis spectrophotometer (Agilent Technologies, America) according to standard methods (SEPA, 2002). The pH and oxidation reduction potential (ORP) were measured with pH/ORP/Conductivity meter (Seven-Multi, Mettler-Toledo Instruments Co., Ltd., China). Residual chlorine was analyzed using a pocket chlorimeter (RC-3F, Dai li co., Ltd) according to DPD (N,N-diethyl-p-phenylenediamine) method.
10
Measuring pH, Conductivity, and Redox Potential
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The pH, σ, and Eh of the MVN and illite-PstHO were measured using three probes (Inlab Expert Pro pH, Inlab Redox, and Inlab 731, respectively) on a Mettler Toledo SevenMulti™ (Mettler Toledo, Columbus, OH, USA) [53 ].
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