Electrically heated chamber

Manufactured by Okolab

The Electrically heated chamber is a laboratory equipment designed to provide a controlled temperature environment for various applications. It operates using electric heating elements to maintain the desired temperature within the enclosed chamber. The core function of this product is to create a stable and consistent temperature-controlled setting for experiments, testing, or other temperature-sensitive processes.

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Lab products found in correlation

Eclipse ti e, by Nikon (2 mentions) Cfi apo tirf 100 oil, by Nikon (1 mentions) Cfi apo tirf 100, by Nikon (1 mentions)

2 protocols using electrically heated chamber

Single-Molecule Microscopy of CRISPR-Cas9

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All single-molecule assays were carried out on an inverted microscope (Nikon Eclipse Ti-E) fitted with a CFI Apo TIRF 100× oil-immersion objective (N.A. 1.49, Nikon). The temperature was maintained at 31.2 °C by an electrically heated chamber (Okolab). dsDNA was visualized every 10 s for 30 min by exciting the Sytox Orange. with a 568-nm laser (Coherent, Sapphire 568–200 CW) at 80 mW/cm². The red fluorescently labeled gRNA was excited at 80 mW/cm² with a 647-nm laser (Coherent, Obis 647–100 CW). The AF488–dCas9 was visualized with a 488-nm laser (Coherent, Sapphire 488–200 CW) at 140 mW/cm². The signals were spectrally separated using appropriate filter sets (Chroma) and fluorescence signals collected on an Evolve 512 Delta EMCCD (Photometics). Typically, nine fields of view were selected for imaging. Single-molecule experimental results were derived from at least three or four technical replicates for each experimental condition.

Schauer G.D., Spenkelink L.M., Lewis J.S., Yurieva O., Mueller S.H., van Oijen A.M, & O’Donnell M.E. (2020). Replisome bypass of a protein-based R-loop block by Pif1. Proceedings of the National Academy of Sciences of the United States of America, 117(48), 30354-30361.

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Single-Molecule Live-Cell Imaging

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All single-molecule assays were carried out on an inverted microscope (Nikon Eclipse Ti-E) fitted with a CFI Apo TIRF 100 × oil-immersion objective (NA 1.49, Nikon). The temperature was maintained at 31.2°C by an electrically heated chamber (Okolab). dsDNA was visualized every 10 s for 30 min by exciting the S.O. with a 568-nm laser (Coherent, Sapphire 568–200 CW) at 80 mW/cm². The red fluorescently labeled proteins were excited at 80 mW/cm² (800 W/cm² during a FRAP pulse) with a 647-nm laser (Coherent, Obis 647–100 CW). The AF488–Pol ε was visualized with a 488-nm laser (Coherent, Sapphire 488–200 CW) at 140 mW/cm². The signals were spectrally separated using appropriate filter sets (Chroma) and fluorescence signals collected on an Evolve 512 Delta EMCCD (Photometics). Typically, nine fields of view (five for the FRAP experiments) were selected for imaging. Single-molecule experimental results were derived from at least three or four technical replicates for each experimental condition.

Lewis J.S., Spenkelink L.M., Schauer G.D., Yurieva O., Mueller S.H., Natarajan V., Kaur G., Maher C., Kay C., O’Donnell M.E, & van Oijen A.M. (2019). Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication. Molecular cell, 77(1), 17-25.e5.

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