Lab chart pro software v8

Experiments were performed using standard organ bath technique as described previously.^{25 (link)} Freshly excised distal colon was quickly placed in a Petri dish containing physiological Krebs’ solution. The colonic segment marked by Indian ink was cut into a 5-mm ring. The colonic rings were then mounted between 2 small metal hooks attached to force displacement transducers in a muscle strip myograph bath (Model 820 MS; Danish Myo Technology, Aarhus, Denmark) containing 7 mL of physiological Krebs’ solution (oxygenated with 95% O₂ and 5% CO₂) maintained at 37 °C. Then, the rings were stretched to give a basal tension of 0.5 g and were equilibrated for 60 min in Krebs’ solution changed at every 20 min. Force contraction of the circular smooth muscle was recorded and analyzed by using a Power Lab 16/35 data acquisition system (ADInstruments, NSW, Australia) and a computer via Lab Chart Pro Software v8.1.16 (ADInstruments, NSW, Australia). Tissue viability and integrity were checked by eliciting contraction response to 60 mM KCl. Colon segments were stimulated with pulse trains of 10-50 V for 30 s, with the pulse duration of 300 µs, at a frequency of 5 Hz by using a CS4+ constant voltage stimulator with Myo Pulse software (Danish Myo Technology, Aarhus, Denmark).

Experiments were performed using the standard organ bath technique as described previously^{23 (link),24 (link)}. Freshly excised colon was quickly placed in a Petri dish containing physiological Krebs solution. The colonic segment marked by Indian ink was cut into a 5 mm ring and mounted between two small metal hooks attached to force displacement transducers in a muscle strip myograph bath (Model 820 MS; Danish Myo Technology, Aarhus, Denmark) containing 7 ml of physiological Krebs solution (oxygenated with 95% O2 and 5% CO2) maintained at 37 °C. The rings were gently stretched to give a basal tension of 0.5 g and were equilibrated for 60 min in Krebs solution changed every 20 min. Colon segments were stimulated with pulse trains of 10–50 V for 30 s, with pulse duration of 300 µs, at a frequency of 5 Hz using a CS4+ constant voltage stimulator with Myo Pulse software (Danish Myo Technology, Aarhus, Denmark). Force contraction of the circular smooth muscle was recorded and analyzed using a Power Lab 16/35 data acquisition system (ADInstruments, NSW, Australia) and Lab Chart Pro Software v8.1.16 (ADInstruments). Acetylcholine (ACh, 100 µM, Sigma) was added to the organ bath to measure maximum contraction. Tissue viability and integrity were checked by eliciting contraction response to 60 mM KCl at the end of the study.