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3 protocols using bml 210

1

Biochemical Inhibitors for Cell Signaling

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Okadaic Acid (10011490), TMP269 (17738), MC 1568 (16265), BML-210 (10005019), MK-2206(11593) were purchased from Cayman. DT-061 (S8774) was purchased from SELLECK CHEMICALS. 14-3-3 Antagonist I, 2–5 (100081) was purchased from Sigma.
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2

Mechanisms of Cytoskeletal Regulation

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The following chemicals were used: Ionomycin (Cayman, 10004974), BAPTA (Invitrogen, B6769), Thapsigargin (Invitrogen, T7459), Paclitaxel (VWR, T104-0005), Colchicine (Sigma, C9754), Nocodazole (Sigma, M1404), C646 (Cayman, 328968-36-1), VIVIT (Cayman, 249537-73-3), BML 210 (Cayman, 537034-17-6), Fluo-4 AM (Introgen, F14201), SMIFH2 (Tocris, 4401), MG-132 (Sellekchem, S2619) and Chloroquine (Sigma, C6628).
The following primary antibodies were used for immunoblotting and co-immunoprecipitation: GEF-H1 (Cell Signaling, 4076S), acetylated a-tubulin (Santa Cruz, sc-23950), a-tubulin (Sigma, T6074), a-tubulin (Santa Cruz, sc-5274), GAPDH (Santa Cruz, sc-20357), Phospho Myosin Light Chain 2 (Cell Signaling, 3674S), Myosin Light Chain 2 (Cell Signaling, 8505S), mDia1 (Cell Signaling, 5486S), rabbit IgG (Santa Cruz, sc-2027). The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare, NA931).
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3

Mechanisms of Cytoskeletal Regulation

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The following chemicals were used: Ionomycin (Cayman, 10004974), BAPTA (Invitrogen, B6769), Thapsigargin (Invitrogen, T7459), Paclitaxel (VWR, T104-0005), Colchicine (Sigma, C9754), Nocodazole (Sigma, M1404), C646 (Cayman, 328968-36-1), VIVIT (Cayman, 249537-73-3), BML 210 (Cayman, 537034-17-6), Fluo-4 AM (Introgen, F14201), SMIFH2 (Tocris, 4401), MG-132 (Sellekchem, S2619) and Chloroquine (Sigma, C6628).
The following primary antibodies were used for immunoblotting and co-immunoprecipitation: GEF-H1 (Cell Signaling, 4076S), acetylated a-tubulin (Santa Cruz, sc-23950), a-tubulin (Sigma, T6074), a-tubulin (Santa Cruz, sc-5274), GAPDH (Santa Cruz, sc-20357), Phospho Myosin Light Chain 2 (Cell Signaling, 3674S), Myosin Light Chain 2 (Cell Signaling, 8505S), mDia1 (Cell Signaling, 5486S), rabbit IgG (Santa Cruz, sc-2027). The following secondary antibodies were used for immunoblotting: ECL rabbit HRP-linked IgG (GE Healthcare, NA934) and ECL mouse HRP-linked IgG (GE Healthcare, NA931).
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