Rpmi 1640 glutamax supplement

RPMI 1640 Glutamax Supplement (Thermo Fisher Scientific, Waltham, MA, United States), 10% Human Serum (BIOWEST, Riverside, MO, United States), 1mM Na pyruvate (Thermo Fisher Scientific, Waltham, MA, United States), 10mM/ml Hepes (Thermo Fisher Scientific, Waltham, MA, United States), 1X MEM Non-Essential Amino Acids (Thermo Fisher Scientific, Waltham, MA, United States), 0.1% β-mercaptoethanol and 1% penicillin-streptomycin (Bioconcept, Allschwil/BL, Switzerland).

Jurkat (DSMZ, ACC 282) and DND41 (DSMZ, ACC 525) cells were cultured in RPMI 1640 GlutaMAX supplement (Thermo Fisher, 61870044) with 10% FBS. Cells were maintained in suspension culture at a density between 0.3 × 10⁶ and 1 × 10⁶. Cells were split at a ratio of 1:2 to 1:4 every 2–4 d and were frozen in 70% RPMI medium, 20% FBS and 10% dimethylsulfoxide. PEER (ACC6, DSMZ), PER-117 (a gift from U. Kees, Perth), MOLT-16 (ACC29, DSMZ), RPMI-8402 (ACC290, DSMZ), LOUCY (ACC394, DSMZ), TALL-1 (ACC521, DSMZ), ALL-SIL (ACC511, DSMZ), NALM-6 (ACC128, DSMZ), NALM-16 (ACC680, DSMZ), MHH-CALL-2 (ACC341, DSMZ), MHH-CALL-4 (ACC337, DSMZ) and MUTZ5 (ACC490, DSMZ) were maintained in RPMI 1640 medium containing 10% or 20% FBS (HyClone), penicillin/streptomycin (100 U ml⁻¹) and glutamine (100 µM) at 37 °C, 5% CO₂. Cell identity was confirmed by short tandem repeat profiling using a PowerPlex Fusion System (Promega). All cell lines were confirmed as free from Mycoplasma spp. using the Universal Mycoplasma Detection kit (American Type Culture Collection).