Zymosan (tlrl-zyn), Pam3CSK4 (tlrl-pms) were purchased from Invivogen. DPI(D2926), NAC(A9165), Cycloheximide(C7698), DHE(D7008), Amplex red and peroxidase were purchased from Sigma. DCFH-DA and pHrodo Red Zymosan A Bioparticles conjugate (P35364) were obtained from Molecular probe. For western blot analysis, specific antibodies for p-IKKα/β (2697), IKKβ (8943), p-JNK (4668), JNK (9258), p-NF-κB p65 (3033), NF-κB p65 (4764), p-Erk1/2 (9101), Erk1/2 (9102), p-p38 (4631), p38 (9212), p-IκBα (2859), IκBα (4812) and p67phox (3923) were purchased from Cell Signaling Technology. Antibodies for FAF1 (sc-393965), p47phox (sc-14015), IKKα (sc-7606), β-actin (sc-47778), GST (sc-138) were purchased from Santa Cruz Biotechnology. Anti-p67phox antibody (ab109366) was purchased from abcam. Anti-V5 antibody (46–0705) was purchased from Invitrogen life technology. The anti-FAF1 monoclonal antibody was kindly provided by Dr. Eunhee Kim (Chungnam National University, Daejeon, Korea).
Anti v5 antibody 46 0705
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-V5 antibody (46–0705) is a laboratory product used for the detection and purification of proteins tagged with the V5 epitope. It is a mouse monoclonal antibody that specifically binds to the V5 tag, allowing researchers to identify and isolate V5-tagged proteins from various samples.
Automatically generated - may contain errors
Lab products found in correlation
Dcfh da, by Thermo Fisher Scientific (1 mentions)
Plus reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine, by Thermo Fisher Scientific (1 mentions)
Ab9049, by Abcam (1 mentions)
Ab7315, by Abcam (1 mentions)
Sinefungin, by Merck Group (1 mentions)
Actin a2066, by Merck Group (1 mentions)
Gapdh sc 166574, by Santa Cruz Biotechnology (1 mentions)
R pfi 2, by Merck Group (1 mentions)
Ab16048, by Abcam (1 mentions)
2 protocols using anti v5 antibody 46 0705
1
Zymosan and Pam3CSK4 Induced Signaling
2
Antibodies and Reagents for Cellular Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies against SRF (sc-335), MyoD (sc-304), Set7/9 (sc-390823), MCK (sc-15164), and GAPDH (sc-166574) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Antibodies against Flag (F1804), HA (H9658), GFP (G1544), and actin (A2066) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against histone H3 (trimethyl K4, ab8580), histone H3 (di-methyl K36, ab9049), pan-methyl lysine (ab7315), and lamin B1 (ab16048) were from Abcam (Cambridge, UK). Anti-KDM2B/JHDM1B antibody (09-864) was from EMD Millipore Corp (Billerica, MA, USA). Anti-MHC antibody (MF-20) was from DSHB (Iowa City, IA, USA). Anti-V5 antibody (46-0705) was from Invitrogen (Carlsbad, CA, USA). SRF-derived peptides (1~5, K154, K163, K165, K165A) were synthesized by Peptron (Daejeon, South Korea). Sinefungin and (R)-PFI-2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). C2C12 cells were transfected using lipofectamine and Plus reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's directions. Each recombinant expression vector was transiently transfected into HEK293T cells with PEI reagents according to the manufacturer's instructions. The siRNA against KDM2B was obtained from Bioneer (Daejeon, South Korea).
Antibodies against Flag (F1804), HA (H9658), GFP (G1544), and actin (A2066) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against histone H3 (trimethyl K4, ab8580), histone H3 (di-methyl K36, ab9049), pan-methyl lysine (ab7315), and lamin B1 (ab16048) were from Abcam (Cambridge, UK). Anti-KDM2B/JHDM1B antibody (09-864) was from EMD Millipore Corp (Billerica, MA, USA). Anti-MHC antibody (MF-20) was from DSHB (Iowa City, IA, USA). Anti-V5 antibody (46-0705) was from Invitrogen (Carlsbad, CA, USA). SRF-derived peptides (1~5, K154, K163, K165, K165A) were synthesized by Peptron (Daejeon, South Korea). Sinefungin and (R)-PFI-2 were purchased from Sigma-Aldrich (St. Louis, MO, USA). C2C12 cells were transfected using lipofectamine and Plus reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's directions. Each recombinant expression vector was transiently transfected into HEK293T cells with PEI reagents according to the manufacturer's instructions. The siRNA against KDM2B was obtained from Bioneer (Daejeon, South Korea).
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