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Mini beadbeater 8k cell disrupter

Manufactured by Biospec
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The Mini-Beadbeater-8K Cell Disrupter is a laboratory equipment designed to homogenize and disrupt a variety of biological samples. It uses high-speed agitation with small beads to efficiently break down cell walls and membranes. The device can process up to 8 samples simultaneously.

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Lab products found in correlation

Qubit fluorometer, by Thermo Fisher Scientific (3 mentions) Nanodrop lite, by Thermo Fisher Scientific (3 mentions) Zirconia silica bead, by Biospec (2 mentions) Phenol chloroform iaa, by Thermo Fisher Scientific (2 mentions) Glass bead, by Biospec (2 mentions) Taq polymerase, by Takara Bio (1 mentions) Dntp mixture, by Takara Bio (1 mentions) Phenol chloroform isoamyl alcohol, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Takara Bio (1 mentions) Ex taq buffer, by Takara Bio (1 mentions)

Spelling variants of this product

Mini-Beadbeater (545 citations) Mini-beadbeater cell disrupter (4 citations)

5 protocols using mini beadbeater 8k cell disrupter

1

Bacterial DNA Extraction from Cecal and Fecal Samples

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Bacterial DNA was extracted from cecal and fecal contents following the method previously described and in accordance with standardized Earth Microbiome Project protocols (www.earthmicrobiome.org/emp-standard-protocols/). In brief, tissues were dissolved in an extraction buffer (50 mg tissue/ml buffer, 50 mM Tris (pH 7.4), 100 mM EDTA (pH 8.0), 400 mM NaCl, 0.5% w/v SDS) containing 0.4 mg/ml of proteinase K. Upon the addition of glass beads with a diameter of 0.1 mm (500 µl/ml buffer, BioSpec Products), microbial cells were lysed using a Mini-Beadbeater-8K Cell Disrupter (BioSpec Products), followed by overnight incubation in a water bath at 55 °C. Total DNA was subsequently extracted using a Phenol:Chloroform:IAA mixture (25:24:1 v/v, pH 8.0, Ambion), following the manufacturer's guidelines. The DNA yield was quantified, and its quality was determined using a combination of Nanodrop Lite (Thermo Fisher) and Qubit® fluorometer (Invitrogen) assessments.
Saha P., Weigle I.Q., Slimmon N., Poli P.B., Patel P., Zhang X., Cao Y., Michalkiewicz J., Gomm A., Zhang C., Tanzi R.E., Dylla N., Al-Hendy A, & Sisodia S.S. (2024). Early modulation of the gut microbiome by female sex hormones alters amyloid pathology and microglial function. Scientific Reports, 14, 1827.
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2

Bacterial DNA Isolation and 16S rRNA Amplification

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Isolation of bacterial DNA was performed as previously described.23 In brief, mouse stool and cecal contents were collected and placed in 1mL of T.N.E.S. extraction buffer. After addition of 0.1-mm-diameter zirconia/silica beads (BioSpec Products, Bartlesville, OK, USA), samples were disrupted using a Mini-Beadbeater-8k Cell Disrupter (BioSpec Products) and incubated overnight at 55°C. Supernatants were then extracted with an equal volume of Phenol:Chloroform:Isoamylalcohol (25:24:1; Ambion, Austin, TX, USA) and DNA precipitated using an equal volume of 100% ethanol. The resulting pellet was centrifuged (13,000 rpm for 5 min), washed with 70% ethanol, dried, and reconstituted in nuclease-free water.
Polymerase chain reaction was performed as follows: 5µL of 10× Ex Taq buffer containing 20mM MgCl2 (Takara, Tokyo, Japan), 4µL of 2.5mM dNTP Mixture (Takara), 1µL each of FAM-labeled forward (27F, 5'-AGA GTT TGA TCC TGG CTC AG-3') and reverse (1492R, GGT TAC CTT GTT ACG ACT-3') primer (10mM each), 0.25µL of Taq polymerase (Takara), 36.75µL nuclease-free water, and 2µL of DNA template. The PCR conditions were: 94°C for 5 min followed by 30 cycles of amplification consisting of denaturation at 94°C for 30 sec, annealing at 58°C for 1 min, and extension at 72°C for 1.5 min.
Huang E.Y., Inoue T., Leone V.A., Dalal S., Touw K., Wang Y., Musch M.W., Theriault B., Higuchi K., Donovan S., Gilbert J, & Chang E.B. (2015). Using Corticosteroids to Reshape the Gut Microbiome: Implications for Inflammatory Bowel Diseases. Inflammatory bowel diseases, 21(5), 963-972.
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3

Bacterial DNA Extraction from Gut Samples

Cited 1 time
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Extraction of bacterial DNA from cecal and fecal contents was performed as previously described44 (link) and conforms to standardized earth microbiome project protocols (www.earthmicrobiome.org/emp-standard-protocols/). Briefly, tissues were dissolved in extraction buffer (50 mg tissue/ml buffer, 50 mM Tris (pH 7.4), 100 mM EDTA (pH 8.0), 400 mM NaCl, 0.5% w/v SDS) containing proteinase K (0.4 mg/ml). After addition of 0.1-mm diameter glass beads (500 ul/ml buffer, BioSpec Products), microbial cells were lysed using a Mini-Beadbeater-8K Cell Disrupter (BioSpec Products) and overnight water bath incubation (55 °C). Total DNA was then extracted using Phenol:Chloroform:IAA (25:24:1 v/v, pH 8.0, Ambion) according to manufacturer’s protocols. DNA yield was quantified and quality ascertained by a combination of Nanodrop Lite (Thermo Fisher) and Qubit® fluorometer (Invitrogen) assessment.
Minter M.R., Zhang C., Leone V., Ringus D.L., Zhang X., Oyler-Castrillo P., Musch M.W., Liao F., Ward J.F., Holtzman D.M., Chang E.B., Tanzi R.E, & Sisodia S.S. (2016). Antibiotic-induced perturbations in gut microbial diversity influences neuro-inflammation and amyloidosis in a murine model of Alzheimer’s disease. Scientific Reports, 6, 30028.
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4

Bacterial DNA Extraction from Caecal and Faecal Samples

Cited 1 time
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Extraction of bacterial DNA from caecal and faecal contents was performed as previously described73 (link) and conforms to standardised earth microbiome project protocols (www.earthmicrobiome.org/emp-standard-protocols/). Briefly, tissues were dissolved in extraction buffer (50 mg tissue/ml buffer, 50 mM Tris (pH 7.4), 100 mM EDTA (pH 8.0), 400 mM NaCl, 0.5% w/v SDS) containing proteinase K (0.4 mg/ml). After addition of 0.1-mm diameter glass beads (500ul/ml buffer, BioSpec Products), microbial cells were lysed using a Mini-Beadbeater-8K Cell Disrupter (BioSpec Products) and overnight water bath incubation (55 °C). Total DNA was then extracted using Phenol:Chloroform:IAA (25:24:1 v/v, pH 8.0, Ambion) according to manufacturer’s protocols. DNA yield was quantified and quality ascertained by a combination of Nanodrop Lite (Thermo Fisher) and Qubit® fluorometer (Invitrogen) assessment.
Minter M.R., Hinterleitner R., Meisel M., Zhang C., Leone V., Zhang X., Oyler-Castrillo P., Zhang X., Musch M.W., Shen X., Jabri B., Chang E.B., Tanzi R.E, & Sisodia S.S. (2017). Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APPSWE/PS1ΔE9 murine model of Alzheimer’s disease. Scientific Reports, 7, 10411.
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5

Stool DNA Extraction Protocol

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For DNA extraction, 50 mg stool samples were dissolved in 1 mL lysis buffer (50 mmol/L Tris [pH 7.4], 100 mmol/L EDTA [pH 8.0], 400 mmol/L NaCl, 0.5% SDS; salts and chemical reagents from Thermo Fisher, Itasca, IL) containing 20 μL proteinase K (20 mg/mL). About 500 μL slurry of 0.1 mm diameter zirconia/silica beads (BioSpec Products, Bartlesville, OK) were added into the extraction tubes and a Mini‐Beadbeater‐8k Cell Disrupter (BioSpec) used to lyse the bacteria. After overnight incubation at 55°C, standard DNA extraction with phenol:chloroform:isoamyl alcohol and precipitation with ethanol were performed. Isolated DNA was dissolved in nuclease‐free water and stored at −80°C.
Hasebe T., Ueno N., Musch M.W., Nadimpalli A., Kaneko A., Kaifuchi N., Watanabe J., Yamamoto M., Kono T., Inaba Y., Fujiya M., Kohgo Y, & Chang E.B. (2016). Daikenchuto (TU‐100) shapes gut microbiota architecture and increases the production of ginsenoside metabolite compound K. Pharmacology Research & Perspectives, 4(1), e00215.
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