B6 cg sting1tm1.2camb j

Manufactured by Jackson ImmunoResearch

B6(Cg)-Sting1tm1.2Camb/J is a genetically modified mouse strain from Jackson ImmunoResearch. It carries a targeted mutation in the Sting1 gene.

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4 protocols using b6 cg sting1tm1.2camb j

Generation and Maintenance of Genetically Engineered Mouse Strains

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WT (C57BL6/J; RRID:IMSR_JAX:000664), IFNAR KO (B6.129S2-Ifnar1^tm1Agt/Mmjax, RRID:MGI:3703445), STING KO (B6(Cg)-Sting1^tm1.2Camb/J, RRID:IMSR_JAX:025805), and IL10 KO (B6.129P2-Il10tm1Cgn/J, RRID:IMSR_JAX:002251) strains of Mus musculus were obtained from The Jackson Laboratory. MyD88-TRIF double KO mice were generated from the two strains MyD88 KO (B6.129P2(SJL)-Myd88^tm1.1Defr/J, RRID:IMSR_JAX:009088) and TRIF KO (C57BL/6J-Ticam1^Lps2/J, RRID:IMSR_JAX:005037). All KO mouse experiments used only homozygous animals. All mice were housed and maintained in specific pathogen–free conditions at the Seattle Children’s Research Institute (SCRI). All experiments were approved by the Institutional Animal Care and Use Committee and then performed in compliance with the relevant protocols. Healthy eight- to 14-week-old female mice without any previous procedure history were used for all experiments and were age-matched within each experiment.

Olson G.S., Murray T.A., Jahn A.N., Mai D., Diercks A.H., Gold E.S, & Aderem A. (2021). Type I interferon decreases macrophage energy metabolism during mycobacterial infection. Cell reports, 35(9), 109195.

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Conditional Knockout Mouse Models for Immunology Research

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C57BL/6J WT mice, B6(Cg)-Sting1^tm1.2Camb/J (STING^–/–) mice, B6.129S7-Rag1^tm1Mom/J (Rag1^–/–) mice, B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (Cd4^cre) mice, B6.129-Prdm1^tm1clme/J (Prdm1^fl/fl) mice, B6.Cg-Tg(Prdm1-EYFP)1Mnz/J (Blimp1-eYFP) mice, and B6.129S2-Ifnar1^tm1Agt/Mmjax (IFNAR^–/–) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). CD4^creBlimp1^fl/fl mice were generated by crossing Cd4^cre mice with Prdm1^fl/fl mice. All the above mice were maintained in the specific pathogen-free animal facility, and GF C57BL/6 mice were bred and maintained in the GF mouse facility of the University of Texas Medical Branch. All the mouse experiments in this study were reviewed and approved by the Institutional Animal Care and Use Committee.

Yang W., Yu T., Zhou G., Yao S., Wakamiya M., Hu H., Paessler S., Sun J, & Cong Y. (2023). Intrinsic STING Switches off Pathogenetic Programs of Th1 Cells to Inhibit Colitis. Cellular and Molecular Gastroenterology and Hepatology, 15(5), 1161-1179.

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Generating cGAS-Deficient Mice for Colon Cancer Research

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cGas^−/− mice were generated as previously reported (48 (link)). WT C57BL/6J, B6.SJL-Ptprc^aPepc^b/BoyJ, B6(Cg)-Sting1tm1.2Camb/J, C57BL/6J-Sting1gt/J and Lgr5-EGFP-IRES-creERT2 mice were purchased from the Jackson Laboratory. The Apc(min/+) and IFNAR^−/− mice were kindly provided, respectively, by Joshua Mendell and David Farrar, University of Texas Southwestern, Dallas, TX. All of these strains were maintained on C57BL6/J background and bred in a specific pathogen-free facility. To rule out the contribution of microbiota in influencing colon inflammation and cancer development, WT and cGas^−/− mice were cohoused for at least 2 wk for all experiments; in some cases, where indicated, the mice were cohoused throughout the duration of the experiments. All study protocols were approved by the Institutional Care and Use Committee of the University of Texas Southwestern Medical Center and were conducted in accordance with the Institutional Animal Care and Use Committee guidelines and the NIH Guide for the Care and Use of Laboratory Animals (49 ). All the experiments were conducted with sex and age-matched mice and both male and female mice were included.

Hu S., Fang Y., Chen X., Cheng T., Zhao M., Du M., Li T., Li M., Zeng Z., Wei Y., Gu Z., Zhang C., Sun L, & Chen Z.J. (2021). cGAS restricts colon cancer development by protecting intestinal barrier integrity. Proceedings of the National Academy of Sciences of the United States of America, 118(23), e2105747118.

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Murine Models for Immunological Studies

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Female C57BL/6J (stock 000664), B6.Cg-Ptprc^aPepc^b Tg(TcrLCMV)1Aox/Ppmj (SMARTA-1; stock 030450), B6.129S(C)-Batf3^tm1Kmm/J (Batf3 knockout; stock 013755), B6(Cg)-Sting1^tm1.2Camb/J (Sting-1 knockout; stock 025805), B6.129S6-Clec7a^tm1Gdb/J (Dectin-1 knockout; stock 012337), B6.129P2(SJL)-Myd88^tm1.1Defr/J (Myd88 knockout, stock 009088), C57BL/6J-Ticam1Lps2/J (Trif knockout, stock 005037), B6.129-Mavs^tm1Zjc/J (MAVS knockout, stock 008634), B6.Cg-Ifih1^tm1.1Cln/J (Mda5 knockout, stock 015812), B6.129S2-H2dlAb1-Ea/J (MHC class II knockout; stock 003584), Il17a^{tm1.1(icre)Stck}/J (IL-17a knockout; stock 016879), B6.129P2-Rorc^tm1Litt/J (RORγt knockout; stock 007571) and B6.129S2-Cd8a^tm1Mak/J (CD8a knockout; 002665) mice were obtained from the Jackson Laboratories. OT-II⁺ TCRα^−/− CD45.1^+/+(OT-II) mice were kindly provided by Arlene Sharpe (Harvard Medical School, Boston, USA). Animals were housed in specific pathogen free and BSL2 conditions at The University of Chicago, and all experiments were performed in accordance with the US National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by The University of Chicago Institutional Animal Care and Use Committee.

Pandey S., Gruenbaum A., Kanashova T., Mertins P., Cluzel P, & Chevrier N. (2020). Pairwise Stimulations of Pathogen-Sensing Pathways Predict Immune Responses to Multi-Adjuvant Combinations. Cell systems, 11(5), 495-508.e10.

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