Celltiter fluor cell viability

FXR-bla HEK293 cells, components of individual thaw media, growth media, assay media, beta-lactamase assay reagents, and coactivator assays for CAR, FXR, and LXRα were purchased from Life Technologies (Carlsbad, CA, USA). CAR-luc cells were generated as described previously (Lynch et al., 2015 (link)). LXRα-luc and PXR-luc assays were acquired from Indigo Biosciences (State College, PA, USA) and Puracyp (Carlsbad, CA, USA), respectively. Compound solutions were prepared from NCATS’ in-house compound collections (e.g., Tox21 10K library). Powder compounds were purchased from SelleckChem (Houston, TX, USA) and Sigma-Aldrich (St. Louise, MO, USA) and sample quality control (QC) analysis was measured on Agilent LC/UV/MS system (Santa Clara, CA, USA) using a standard gradient of 4-100% acetonitrile in the presence of 0.05% trifluoroacetic acid over seven minutes on a 3 micron Luna C18 (3×75 mm) column. All 1536-well compound storage and assay plates were obtained from Greiner Bio One USA (Monroe, NC, USA). CellTiter-Fluor cell viability, CellTiter-Glo cell viability, and One-Glo luciferase assay reagents were acquired from Promega (Madison, WI, USA).