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8wg16 ab817

Manufactured by Abcam
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The 8WG16 (ab817) is a laboratory equipment product offered by Abcam. It serves as a core component in various experimental setups. The product specification and technical details are available upon request.

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Lab products found in correlation

Normal mouse igg, by Merck Group (2 mentions) Sonicator, by Qsonica (2 mentions) Formaldehyde, by Merck Group (2 mentions) Dynabeads protein g, by Thermo Fisher Scientific (2 mentions) Abi 7500, by Thermo Fisher Scientific (2 mentions) Sybr green, by Bio-Rad (2 mentions)

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Ab817 (55 citations)

2 protocols using 8wg16 ab817

1

Chromatin Immunoprecipitation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were essentially performed as previously described51 (link) with modifications: 5×106 cells were fixed with formaldehyde (1% final volume concertration, Sigma), 10 min at room temperature. Fixation was stopped by addition of 1/10 volume 1.25M glycine and incubated for 5min at room temperature. The sonication step was performed in a Qsonica sonicator (5min, 20s on, 20s off), and 200μg of protein-chromatin complex was used for each immunoprecipitation. Antibody-protein complex was captured with preblocked dynabeads protein G (Invitrogen). ChIP DNA was analyzed by qPCR with SYBR Green (Biorad) in ABI-7500 (Applied Biosystems) using the primers specified in Supplemental Table S4. The antibodies used are as followed: 8WG16 (ab817, Abcam), normal mouse IgG (12-371, Millipore).
Jiang Y., Yan B., Lai W., Shi Y., Xiao D., Jia J., Liu S., Li H., Lu J., Li Z., Chen L., Chen X., Sun L., Muegge K., Cao Y, & Tao Y. (2015). Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene, 34(50), 6079-6091.
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2

Chromatin Immunoprecipitation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were essentially performed as previously described51 (link) with modifications: 5×106 cells were fixed with formaldehyde (1% final volume concertration, Sigma), 10 min at room temperature. Fixation was stopped by addition of 1/10 volume 1.25M glycine and incubated for 5min at room temperature. The sonication step was performed in a Qsonica sonicator (5min, 20s on, 20s off), and 200μg of protein-chromatin complex was used for each immunoprecipitation. Antibody-protein complex was captured with preblocked dynabeads protein G (Invitrogen). ChIP DNA was analyzed by qPCR with SYBR Green (Biorad) in ABI-7500 (Applied Biosystems) using the primers specified in Supplemental Table S4. The antibodies used are as followed: 8WG16 (ab817, Abcam), normal mouse IgG (12-371, Millipore).
Jiang Y., Yan B., Lai W., Shi Y., Xiao D., Jia J., Liu S., Li H., Lu J., Li Z., Chen L., Chen X., Sun L., Muegge K., Cao Y, & Tao Y. (2015). Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene, 34(50), 6079-6091.
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