At each time point, the coupons in a single rod were removed and rinsed in sterile distilled water. The coupons were blotted dried on a paper towel biofilm side up. The coupon was placed, film side up, in a 24 well plate containing 370 uL of 0.01% CV in water solution for 15 min at room temperature (RT). The coupons were rinsed in distilled water, blotted dried, and put into a well containing 7 glass beads with the film side down. To the same well, 350 μL 95% ethanol was added and incubated for 30 min at RT. After incubation, 50 μL crystal violet infused ethanol was diluted with 100 μL 95% ethanol in a 96-well plate. This is repeated two more times for a total of three 1:3 dilutions per coupon. The optical densities of the solutions were measured using the Cary 50 UV-Vis Spectrometer microplate reader at 600 nm.
Cary 50 uv vis spectrometer microplate reader
Manufactured by Agilent Technologies
The Cary 50 UV-Vis Spectrometer is a microplate reader that measures the absorbance of samples in microplates. It provides accurate and reliable data for a variety of applications, including quantitative analysis, kinetic studies, and endpoint measurements.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cary 50 uv vis spectrometer microplate reader
1
Biofilm Quantification via Crystal Violet
2
Biofilm Quantification via Crystal Violet
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At each time point, the coupons in a single rod were removed and rinsed in sterile distilled water. The coupons were blotted dried on a paper towel biofilm side up. The coupon was placed, film side up, in a 24 well plate containing 370 uL of 0.01% CV in water solution for 15 min at room temperature (RT). The coupons were rinsed in distilled water, blotted dried, and put into a well containing 7 glass beads with the film side down. To the same well, 350 μL 95% ethanol was added and incubated for 30 min at RT. After incubation, 50 μL crystal violet infused ethanol was diluted with 100 μL 95% ethanol in a 96-well plate. This is repeated two more times for a total of three 1:3 dilutions per coupon. The optical densities of the solutions were measured using the Cary 50 UV-Vis Spectrometer microplate reader at 600 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!