Water used was deionized (18 m Ω) with a MilliQ® Integral 5 system (Merck KGaA). Other commercially available chemicals were reagent grade or higher. Peptide synthesis was performed by New England Peptide, Inc. on solid phase and purified to ≥95%.
Milli q integral 5 system
Manufactured by Merck Group
Sourced in Germany, United States
The Milli-Q Integral 5 system is a water purification device designed to produce high-quality ultrapure water. It utilizes a combination of filtration, ion exchange, and UV treatment technologies to remove impurities and contaminants from the water supply. The system is capable of producing water with a high degree of purity, making it suitable for use in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (4 mentions)
Formic acid, by Merck Group (3 mentions)
Methanol, by Biosolve (2 mentions)
Ethanol, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Ammonium hexafluorophosphate, by Merck Group (2 mentions)
Nanopure water, by Merck Group (2 mentions)
β glucuronidase escherichia coli, by Roche (2 mentions)
Ampicillin sodium salt, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
L glutathione reduced, by Merck Group (1 mentions)
Potato dextrose agar (pda), by Scharlab (1 mentions)
Low molecular weight chitosan, by Merck Group (1 mentions)
Potato dextrose broth (pdb), by Scharlab (1 mentions)
Tryptic soy broth (tsb), by Scharlab (1 mentions)
L glutamate, by Merck Group (1 mentions)
19 protocols using milli q integral 5 system
1
Peptide Synthesis and Purification
2
Silver Nanoparticle Synthesis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silver nitrate (AgNO3, ≥99.0%), L-glutathione reduced (GSH, ≥98.0%), sodium hydroxide (NaOH, ≥98.0%), sodium borohydride (NaBH4, 99%), ethanol (EtOH, ≥99.5%), and ampicillin sodium salt were all purchased from Sigma-Aldrich (Saint Louis, MO, USA). Ultrapure water with a resistivity of 18.2 MΩ × cm (Milli-Q Integral 5 system, Merck Millipore, Billerica, MA, USA) was uitlized in all experiments.
3
Electrochemical Detection of Heavy Metals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tris hydrochloride (Tris-HCl), potassium chloride (KCl), hydrochloric acid (HCl), low molecular weight chitosan (CS, MW = 50–190 kDa), potassium dichromate (K2Cr2O7), lead (II) nitrate Pb(NO3)2, zinc sulfate heptahydrate (ZnSO4·7H2O), potassium ferricyanide (K4Fe(CN)6), ferric chloride (FeCl3), tetrachloroauric(III) acid trihydrate, and sodium citrate were obtained from Sigma-Aldrich, Merck KGaA, Darmstadt, Germany. The multi-walled carbon nanotubes (MWCNT) were purchased from Carbon GmbH, Bayreuth, Germany. The electrochemical measurements for chromium and zinc were carried out in a solution containing 0.1 M HCl and 0.1 M KCl, and those for lead in a 0.1 M Tris-HCl buffer pH 5, respectively. All solutions used in the experiments were prepared using ultrapure water (18.25 MΩ·cm) obtained with a Milli-Q® Integral 5 system, Merck KGaA, Darmstadt, Germany.
Analytical grade HNO3 65%, HCl 37%, and HF 50% for the acid digestion of soil samples were obtained from Thermo Fisher Scientific, Waltham, MA, USA. Standard solutions for chromium, lead, and zinc were obtained from Merck, KGaA, Darmstadt, Germany. NaCl, as well as dehydrated culture media, namely PDA, TSA, PDB, TSB, Luria–Bertani, and Sabouraud, were obtained from Scharlau, Sentmenat, Spain.
Analytical grade HNO3 65%, HCl 37%, and HF 50% for the acid digestion of soil samples were obtained from Thermo Fisher Scientific, Waltham, MA, USA. Standard solutions for chromium, lead, and zinc were obtained from Merck, KGaA, Darmstadt, Germany. NaCl, as well as dehydrated culture media, namely PDA, TSA, PDB, TSB, Luria–Bertani, and Sabouraud, were obtained from Scharlau, Sentmenat, Spain.
4
Synthesis and Characterization of α-KG Derivatives
5
Analytical Standards for Sulfonic Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals were of analytical
grade and used as-received without further purification. Carbon dioxide
Premium (4.5) was used for SFC. Methanol, acetonitrile, and formic
acid were provided by Biosolve (Valkenswaard, Netherlands). A sodium
hypochlorite solution (6–14%) was provided by Th. Geyer GmbH
(Hamburg, Germany). Disodium hydrogen phosphate (≥99%), potassium
dihydrogen phosphate (≥99%), cysteine (≥99%), and glutathione
(99%) were purchased from Merck (Darmstadt, Germany). p-Phenolsulfonic acid hydrate (85%) was supplied by abcr GmbH (Karlsruhe,
Germany). Suwannee River Fulvic Acid (SRFA) was purchased from the
International Humic Substances Society (SRFA II; 2S101H). The in-house
synthesized and purified standards of chloromethanesulfonic acid (ClMSA),
bromomethanesulfonic acid (BrMSA), dichloromethanesulfonic acid (Cl2MSA), bromochloromethanesulfonic acid (BrClMSA), and dibromomethanesulfonic
acid (Br2MSA) were offered by D. Zahn.16 (link) Ultrapure water was obtained from a Merck Milli-Q Integral
5 system (Darmstadt, Germany).
grade and used as-received without further purification. Carbon dioxide
Premium (4.5) was used for SFC. Methanol, acetonitrile, and formic
acid were provided by Biosolve (Valkenswaard, Netherlands). A sodium
hypochlorite solution (6–14%) was provided by Th. Geyer GmbH
(Hamburg, Germany). Disodium hydrogen phosphate (≥99%), potassium
dihydrogen phosphate (≥99%), cysteine (≥99%), and glutathione
(99%) were purchased from Merck (Darmstadt, Germany). p-Phenolsulfonic acid hydrate (85%) was supplied by abcr GmbH (Karlsruhe,
Germany). Suwannee River Fulvic Acid (SRFA) was purchased from the
International Humic Substances Society (SRFA II; 2S101H). The in-house
synthesized and purified standards of chloromethanesulfonic acid (ClMSA),
bromomethanesulfonic acid (BrMSA), dichloromethanesulfonic acid (Cl2MSA), bromochloromethanesulfonic acid (BrClMSA), and dibromomethanesulfonic
acid (Br2MSA) were offered by D. Zahn.16 (link) Ultrapure water was obtained from a Merck Milli-Q Integral
5 system (Darmstadt, Germany).
6
Surface Wettability Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Static deionized water (Milli-Q Integral 5 System, Merck, Darmstadt, Germany) and diiodomethane (Fluka, Thermo Fisher Scientific, Waltham, MA, USA) contact angle measurements were carried out with a Drop Shape Analyzer-DSA100E system (Krüss, Hamburg, Germany) by the sessile drop method. Drops were photographed at 15 s after deposition. The results of contact angles, namely averaged values of at least three independent films, were analyzed for each of the conditions studied and two drops of each liquid were deposited per film.
7
PLA Particles for Antibacterial Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PLA particles PLA2003D, with a D-isomer content of 4.25%, were purchased from NatureWorks LLC (Blair, NE, USA). Chloroform and CTAB were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany) and magnesium particles (≤50 μm) are from Nitroparis (Castellón, Spain). For contact angle measurements, deionized water (Milli-Q Integral 5 System, Merck, Darmstadt, Germany) and diiodomethane (Fluka, Thermo Fisher Scientific, Waltham, MA, USA) were used. For bacterial assays, Tryptic Soy Broth (TSB) medium was purchased from BBL, Becton Dickinson and Company (Sparks, Franklin Lakes, NJ, USA) and viable biofilms were quantified using BacTiter-Glo™ Microbial Cell Viability Assay (Promega Corporation, Madison, WI, USA).
8
Analytical Methodology for Organic Matter
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and reagents used for mobile phase preparation such as methanol, formic acid, acetonitrile (ACN), and ammonium formate were LC‐MS grade and purchased from Sigma-Aldrich (Vienna, Austria). Before measurement, eluents were prepared in LC‐MS grade water containing suitable additives (0.1% v/v formic acid; 10 mol L−1 ammonium formate, pH adjusted to 3.0 with formic acid). HPLC-grade water (MQW) used for the HPLC-TOF-MS was prepared using Milli-Q® IQ 7000 Ultrapure Lab Water System (Merck, Darmstadt, Germany) combined with a Milli-Q LC-Pak® Polisher. The dilution of the exudate samples for DI-FT-ICR-MS was done with methanol purchased from Biosolve, Valkenswaard, the Netherlands, and HPLC-grade water (MQW) generated from a MilliQ Integral 5 system (Merck, Darmstadt, Germany). The organic matter reference sample Suwannee River Fulvic Acid (SRFA II, International Humic Substances Society) was used for quality control during DI-FT-ICR-MS measurement. Details on the chemicals used to prepare soil column experiments can be found in the Supporting Information to Lohse et al. [40 (link)].
9
Supercritical Fluid Chromatography Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC/mass spectrometry (MS)‐grade methanol (MeOH), 2‐propanol (2PrOH) and 1 M ammonium formate (AF) aqueous solution, and analytical grade 28% aqueous ammonium (NH3) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Ultrapure water was produced using a Milli‐Q Integral 5 system (Merck, Darmstadt, Germany). The examined cathinone analogs were synthesized in the laboratory using established methods with some modifications,
25 ,
26 ,
27 and purified sufficiently for instrumental analysis. CO2 of >99.5% purity was purchased from Jyotou Gas (Tokyo, Japan).
Co‐solvents and the make‐up solvent used for SFC were prepared in our laboratory. Organic solvents (MeOH or 2PrOH) and additives (NH3 or AF) were mixed and filtered through a Millicup HV 0.45‐µm polyvinylidene difluoride filter (Merck) before use.
25 ,
26 ,
27 and purified sufficiently for instrumental analysis. CO2 of >99.5% purity was purchased from Jyotou Gas (Tokyo, Japan).
Co‐solvents and the make‐up solvent used for SFC were prepared in our laboratory. Organic solvents (MeOH or 2PrOH) and additives (NH3 or AF) were mixed and filtered through a Millicup HV 0.45‐µm polyvinylidene difluoride filter (Merck) before use.
10
Quantitative Analysis of Clenbuterol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clenbuterol hydrochloride (99.5% purity) and clenbuterol-d 9 (98.5% purity) as the internal standard (IS) were purchased from the FUJIFILM Wako Pure Chemical (Osaka, Japan). Clenbuterol hydrochloride was dissolved in Methanol to prepare the standard stock solution (100 μg/mL) and the solution was stored at -30ºC. Methanol, acetonitrile and formic acid of LC/MS grade were obtained from FUJIFILM Wako.
Anhydrous sodium sulfate, sodium chloride, acetonitrile, acetone and n-hexane of pesticide residue analysis grade and 1-propanol of guaranteed grade were also the products of FUJIFILM Wako. Water was purified by a Milli-Q Integral 5 system (Merck, Darmstadt, Germany).
Anhydrous sodium sulfate, sodium chloride, acetonitrile, acetone and n-hexane of pesticide residue analysis grade and 1-propanol of guaranteed grade were also the products of FUJIFILM Wako. Water was purified by a Milli-Q Integral 5 system (Merck, Darmstadt, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!