Annexin 5 apoptosis assay

Cells were stained with the Annexin V apoptosis assay according to the manufacturer’s protocol (Thermo Fisher Scientific Corporation, Carlsbad, CA, USA). Briefly, cells were detached and washed twice by PBS (without calcium and magnesium) and once in Annexin V binding buffer. Samples were resuspended in 100 μl Annexin V binding buffer and 5 μl Annexin V Alexa Fluor 488 and 1 μl PI was added before incubation in the dark for 15 min at RT. Four hundred microliter Annexin V binding buffer was then added to each sample and the samples were kept on ice and analysed immediately on the Accuri C6 (BD Biosciences) flow cytometer.

Cells were stained with the Annexin V apoptosis assay according to the manufacturer’s protocol (Thermo Fisher Scientific). Samples were analysed on Accuri C6 (BD Biosciences) flow cytometer.