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Mv4 11 cells

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The MV4-11 cell line is a human acute myeloid leukemia (AML) cell line derived from a patient with biphenotypic B myelomonocytic leukemia. The cells are suspension-growing and exhibit myeloid and monocytic characteristics.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (5 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Detection kit, by Lonza (2 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Euroclone (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Thp1 dual cells, by InvivoGen (1 mentions) Thp1 dual ko sting cells, by InvivoGen (1 mentions) Raw 264.7 cells, by American Type Culture Collection (1 mentions) Molm 13 cells, by American Type Culture Collection (1 mentions) M200 pro spectrophotometer, by Tecan (1 mentions) Celltiter glo reagent, by Promega (1 mentions) Enspire multimode plate reader, by PerkinElmer (1 mentions) Ciprofloxacin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Molm 13 cells, by Leibniz Institute DSMZ (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Penicillin streptomycin, by GenDEPOT (1 mentions)

22 protocols using mv4 11 cells

1

AML Cell Line Genetic Manipulation

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AML cells carrying the MLL-ENL translocation (plus IRES-GFP) and oncogenic N-RAS (Luciferase-IRES-N-RASG12D), referred in the text as AMLMLL, were developed as previously described (19 (link)). The retroviral plasmid with a p53-targeting shRNA was provided by Mariano Barbacid and transduced using standard protocols. MV4:11 cells were obtained from ATCC. Cells were cultured in standard conditions (5% CO2 and 20% O2) in RPMI-1640 (Euro Clone) medium supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) and 10 μg/mL peniciline/streptomycin (Pen/Strep; Life Technologies). ATRi (AZ20, synthesized by GVK-BIO), ATMi (AZD0156, Astra Zeneca) and HU (Sigma-Aldrich) were used as indicated.
Morgado-Palacin I., Day A., Murga M., Lafarga V., Anton M.E., Tubbs A., Chen H.T., Ergan A., Anderson R., Bhandoola A., Pike K.G., Barlaam B., Cadogan E., Wang X., Pierce A.J., Hubbard C., Armstrong S.A., Nussenzweig A, & Fernandez-Capetillo O. (2016). Targeting the kinase activities of ATR and ATM exhibits therapeutic potential in a mouse model of MLL-rearranged AML. Science signaling, 9(445), ra91.
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2

Culturing U937, MOLM-13, and MV4-11 Cell Lines

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U937 and MOLM-13 cells were kindly provided by Dr. Wendy Stocks’s lab at the University of Chicago. MV4-11 cells were purchased from ATCC. Cell lines were authenticated at the University of Arizona Cell Authentication Core. Cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium (Thermo Fisher, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin (Thermo Fisher). Primary cells (USC001) were grown in RPMI plus FBS (20%) and cytokine cocktails CC100 (Flt3L, SCF, IL-3 and IL-6).
Vaikari V.P., Park M., Keossayan L., MacKay J.A, & Alachkar H. (2020). Anti-CD99 scFv-ELP nanoworms for the treatment of acute myeloid leukemia. Nanomedicine : nanotechnology, biology, and medicine, 29, 102236.
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3

Diverse Cell Lines and PBMCs Culture

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Human monocyte THP-1 Dual cells (InvivoGen), THP1-Dual™ KO-STING Cells (InvivoGen) and THP1-Dual KI-hSTING-R232 cells (InvivoGen) were cultured in RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin, Pen-Strep, and 10 µg/ml of blasticidin, 100 μg/ml of Zeocin. Mouse RAW 264.7 cells (ATCC) were cultured in DMEM with 10% heat-inactivated fetal bovine serum and Pen-Strep. MV4-11 cells (ATCC) were cultured in ATCC-formulated Iscove's Modified Dulbecco's Medium with 10% heat-inactivated fetal bovine serum and Pen-Strep. MOLM-16 cells (DSMZ) were cultured in RPMI 1640 with 20% heat-inactivated fetal bovine serum. Frozen human peripheral blood mononuclear cells (PBMCs) and Cynomolgous monkey PBMCs were purchased from Stem Cell Technologies and iQ Biosciences respectively.
Song C., Liu D., Liu S., Li D., Horecny I., Zhang X., Li P., Chen L., Miller M., Chowdhury R., Issa M., Shen R., Yan Y., Zhang F., Zhang L., Zhang L., Bai C., Feng J., Zhuang L., Zhang R., Li J., Wilkinson H., Liu J, & Tao W. (2022). SHR1032, a novel STING agonist, stimulates anti-tumor immunity and directly induces AML apoptosis. Scientific Reports, 12, 8579.
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4

MLL Leukemia Patient Bone Marrow Samples

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Bone marrow samples were collected from four MLL leukemia patients at the time of their initial diagnosis, with informed consent obtained from patients treated at the First Affiliated Hospital of Sun Yat-sen University. Ethical approval for sample collection was granted by the Hospital’s Protection of Human Subjects Committee, and detailed clinicopathological characteristics of the patients can be found in Additional file 1: Table S1.
Human THP1 and MOLM-13 cells (ATCC, USA) were cultured in RPMI-1640 medium (HyClone, USA), while MV4-11 cells (ATCC, USA) were cultured in IMDM (HyClone, USA). All cell cultures were supplemented with 10% fetal bovine serum (HyClone, USA) and maintained at 37 °C in a 5% CO2 atmosphere. The primary cells cultured in IMDM (HyClone) supplemented with 20% FBS.
Chen T.Q., Huang H.J., Zhu S.X., Chen X.T., Pu K.J., Wang D., An Y., Lian J.Y., Sun Y.M., Chen Y.Q, & Wang W.T. (2024). Blockade of the lncRNA-DOT1L-LAMP5 axis enhances autophagy and promotes degradation of MLL fusion proteins. Experimental Hematology & Oncology, 13, 18.
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5

MV4-11 Cell Culture Protocol

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MV4-11 cells were purchased from ATCC and used for all in vitro experimentation. Cells were cultured in RPMI based media supplemented with 10% FBS, and kept at 37°C with 5% CO2. Cells were passaged every 2–3 days in order to stay within 1x105-1x106 cells/ml to maintain logarithmic growth.
Wallace J., Hu R., Mosbruger T.L., Dahlem T.J., Stephens W.Z., Rao D.S., Round J.L, & O’Connell R.M. (2016). Genome-Wide CRISPR-Cas9 Screen Identifies MicroRNAs That Regulate Myeloid Leukemia Cell Growth. PLoS ONE, 11(4), e0153689.
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6

Development of Ponatinib and Quizartinib Resistant Leukemia Cell Lines

Cited 1 time
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MOLM13 cells were obtained from the DSMZ (Braunschweig, Germany). MV4-11 cells were obtained from ATCC (Manassas, VA). All experiments with cell lines were performed within 6 months after thawing or obtaining from ATCC or DSMZ. Cell line authentication was performed by ATCC or DSMZ. The ATCC and DSMZ utilize short tandem repeat (STR) profiling for characterization and authentication of cell lines. MOLM13-TKIR and MV4-11-TKIR cells resistant to ponatinib and quizartinib were isolated by exposing the MOLM13 and MV4-11 cells, respectively, to the continuous presence of step-wise escalating levels of ponatinib, utilizing previously described methods (36 (link),37 (link)). Compared to the parental MOLM13 and MV4-11 cells, the TKIR cells were characterized for cell membrane expression of FLT3 and its intracellular signaling, as well for their the cell cycle and suspension culture growth.
Fiskus W., Sharma S., Qi J., Shah B., Devaraj S.G., Leveque C., Portier B.P., Iyer S., Bradner J.E, & Bhalla K.N. (2014). BET protein antagonist JQ1 is synergistically lethal with FLT3 tyrosine kinase inhibitor (TKI) and overcomes resistance to FLT3-TKI in AML cells expressing FLT-ITD. Molecular cancer therapeutics, 13(10), 2315-2327.
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7

Cell Line Authentication and Culture

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MV4–11 cells were purchased from ATCC. MOLM-13 and U937 cells, were kindly provided by Dr. Wendy Stock’s lab. All the cell lines were authenticated at the University of Arizona Cell Authentication Core. All cell lines were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium (Thermo Fisher, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin (Thermo Fisher, MA, USA).
Park M., Vaikari V.P., Lam A.T., Zhang Y., MacKay J.A, & Alachkar H. (2020). For submission at the Journal of controlled release Anti-FLT3 Nanoparticles for acute myeloid leukemia: Preclinical pharmacology and pharmacokinetics. Journal of controlled release : official journal of the Controlled Release Society, 324, 317-329.
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8

Cell Viability Assay for MV4-11 Cells

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MV4–11 cells were purchased from ATCC. The cells were grown according to ATCC protocol in Iscove’s Modified Deulbecco’s Medium with 10% Fetal Bovine Serum. Cells were grown in 37°C environments with 5% CO2. Cells were plated at 20k cells/well in 96-well clear U-bottom plates and pre-incubated for 24 hours. Addition of serially diluted inhibitor (in medium) was performed followed by 48 hours of additional incubation. Addition of CellTiter-Blue occurred to a final concentration of 0.125 mg/mL. The mixture was allowed to incubate until sufficient color changed occurred. Cell viability was measured as a function of resorfuin intensity using a Tecan M200 Pro spectrophotometer, 560 nm (ex.)/590 nm (em.). Data were normalized to control wells and background was removed. EC50 values were determined using GraphPad Prism’s “log(inhibitor) vs. normalized response – Variable slope” function.
McClure J.J., Zhang C., Inks E.S., Peterson Y., Li J, & Chou C.J. (2016). Development of Allosteric Hydrazide-Containing Class I Histone Deacetylase Inhibitors for Use in Acute Myeloid Leukemia. Journal of medicinal chemistry, 59(21), 9942-9959.
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9

Cell Growth Inhibition Assay

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MV4-11 cells (ATCC, Rockville, MD, USA) were cultured in IMDM supplemented with 10% FBS and 1% penicillin–streptomycin at 37 °C in a humidified incubator containing 5% CO2. In the cell growth assay, cells were seeded in 384-well plates at 1000 cells per well in 20 μL of culture medium and cultured for 12 h. Different concentrations of diluted compounds or DMSO control were added into the wells in a volume of 10 µL with the final concentrations from 5 nM to 100 μM and cultured for 120 h. Then, 25 μL CellTiter-GLO reagent (Promega, Madison, WI, USA) was added in each well and mixed on an orbital shaker for 10 min to induce cell lysis. The lysates were incubated for another 10 min and centrifuged for 1 min. Luminescence was measured on an Enspire Multimode Plate Reader (PerkinElmer, Waltham, MA, USA), according to the manufacturer’s instructions. Each concentration point was performed in triplicate. The fluorescence signals were normalized to the DMSO-treated cells, and the inhibitory curves and IC50 values were calculated by nonlinear regression and dose-response inhibition equation analysis using GraphPad Prism 5 software.
Zhang M.F., Luo X.Y., Zhang C., Wang C., Wu X.S., Xiang Q.P., Xu Y, & Zhang Y. (2022). Design, synthesis and pharmacological characterization of N-(3-ethylbenzo[d]isoxazol-5-yl) sulfonamide derivatives as BRD4 inhibitors against acute myeloid leukemia. Acta Pharmacologica Sinica, 43(10), 2735-2748.
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10

Culturing MV4-11 Cells for Research

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MV4-11 cells (ATCC, Manassas, VA, USA) were cultured at the Roswell Park Memorial Institute using RPMI 1640 medium (Gibco Life Technologies, New York, NY, USA) that was supplemented with 4.5 g/L glucose, 2 mM L-glutamine, 10% fetal bovine serum (FBS), and ciprofloxacin (10 µg/mL) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA).
Alkhatabi H.A., Zohny S.F., Shait Mohammed M.R., Choudhry H., Rehan M., Ahmad A., Ahmed F, & Khan M.I. (2022). Venetoclax-Resistant MV4-11 Leukemic Cells Activate PI3K/AKT Pathway for Metabolic Reprogramming and Redox Adaptation for Survival. Antioxidants, 11(3), 461.
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