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Stereo discovery v20 macro stereo

Manufactured by Zeiss
Sourced in Germany

The Stereo Discovery V20 Macro Stereo is a high-performance stereo microscope designed for detailed observation and analysis. It features a 20x optical zoom range, providing a wide field of view and high magnification capabilities. The microscope is equipped with advanced optics and illumination systems to deliver clear and sharp images.

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3 protocols using stereo discovery v20 macro stereo

1

Fluorescent Labeling of Cultured Cells

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Example 4

In a subset of studies, RAECs were labeled with DiI or DiO on the day of recellularization. Briefly, media was removed from a confluent plate of RAECs and replaced with DPBS containing 5 μM SP-DiIC18 or SP-DiOC18 (Invitrogen). After 5 minutes of incubation at 37° C., plates were transferred to a refrigerator and incubated for 15 minutes at 4° C. Plates were then washed once with PBS and allowed to recover for 2 hours at 37° C. in culture media before isolation and construct seeding. At the end of the experiment, constructs were removed from the bioreactor and imaged on a Stereo Discovery V20 Macro Stereo (Carl Zeiss Inc.), dissected, placed in Slowfade (Invitrogen) and imaged on a 510 Meta Confocal microscope (Carl Zeiss Inc.).

In separate studies, RAECs seeded constructs were labeled with Cell Tracker Green CMFDA (Invitrogen) on the last day of culture (Day 7), by removing the complete culture media and circulating serum free CMFDA containing DMEM (Cellgro) for 45 minutes at 37° C. CMFDA containing media was then replaced with complete MCDB-131 and the constructs were incubated for 45 minutes. CMFDA-labeled constructs were then removed from the bioreactor, dissected, placed in Slowfade (Invitrogen) and imaged on a 510 Meta Confocal microscope (Carl Zeiss Inc.).

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2

Fluorescent Labeling of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

In a subset of studies, RAECs were labeled with DiI or DiO on the day of recellularization. Briefly, media was removed from a confluent plate of RAECs and replaced with DPBS containing 5 μM SP-DiIC18 or SP-DiOC18 (Invitrogen). After 5 minutes of incubation at 37° C., plates were transferred to a refrigerator and incubated for 15 minutes at 4° C. Plates were then washed once with PBS and allowed to recover for 2 hours at 37° C. in culture media before isolation and construct seeding. At the end of the experiment, constructs were removed from the bioreactor and imaged on a Stereo Discovery V20 Macro Stereo (Carl Zeiss Inc.), dissected, placed in Slowfade (Invitrogen) and imaged on a 510 Meta Confocal microscope (Carl Zeiss Inc.).

In separate studies, RAECs seeded constructs were labeled with Cell Tracker Green CMFDA (Invitrogen) on the last day of culture (Day 7), by removing the complete culture media and circulating serum free CMFDA containing DMEM (Cellgro) for 45 minutes at 37° C. CMFDA containing media was then replaced with complete MCDB-131 and the constructs were incubated for 45 minutes. CMFDA-labeled constructs were then removed from the bioreactor, dissected, placed in Slowfade (Invitrogen) and imaged on a 510 Meta Confocal microscope (Carl Zeiss Inc.).

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3

Morphological Analysis of CA and EC

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For cell morphological observation, CA, EC, and organ morphological image was captured using Stereo Discovery V20 Macro Stereo (Carl Zeiss, Germany). For cell microscopy observation, fresh CA and EC blocks (<5 mm3) were transferred to a 1.5-ml Eppendorf tube immediately, and the samples were suspended in 0.5 ml of 1% (w/v) acetocarmine for 30 min. Then the CA and EC suspension was diluted with ddH2O, the suspension including CA and EC cells (<0.5 mm3) was transferred to a slide, and a cover glass was placed on the slide slightly. Microscopic observations were performed using an Axio Scope A1 microscope (Carl Zeiss, Germany). The cell diameter was calculated using 10 individual cells.
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