Pro bdnf

Manufactured by Aviscera Bioscience

Sourced in United States

Pro-BDNF is a laboratory product offered by Aviscera Bioscience. It is a precursor form of the brain-derived neurotrophic factor (BDNF) protein. Pro-BDNF is used in research applications to study the biological functions and signaling mechanisms of this important neuronal growth factor.

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Lab products found in correlation

Pro bdnf, by R&D Systems (2 mentions) Mmp 9, by R&D Systems (1 mentions) Sunrise absorbance microplate reader, by Tecan (1 mentions)

2 protocols using pro bdnf

Plasma Biomarker Quantification by ELISA

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Plasma concentrations of MMP-9, BDNF, and pro-BDNF were determined
by ELISA (MMP-9 and BDNF – R&D Systems Inc., Minneapolis, USA; pro-BDNF –
Aviscera Bioscience, Santa Clara, CA, USA) according to the manufacturer’s
protocol. A total of 30 μg/μl of protein from each plasma sample
was diluted 70-fold (MMP-9) or 20-fold (BDNF and pro-BDNF) with calibration
diluent from the assays and analyzed in duplicate. The optical density of wells
was measured at 450 nm using an automated microplate reader (Sunrise Microplate
Absorbance Reader).

Matusiak M., Oziębło D., Ołdak M., Rejmak E., Kaczmarek L., Skarżyński P.H, & Skarżyński H. (2022). Prospective cohort study reveals MMP-9, a neuroplasticity regulator, as a prediction marker of cochlear implantation outcome in prelingual deafness treatment. Molecular Neurobiology, 59(4), 2190-2203.

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Quantification of Plasma Biomarkers

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The plasma concentrations of MMP-9, BDNF, and pro-BDNF were measured using specific enzyme-linked immunosorbent assay (ELISA) kits (MMP-9 and BDNF—R&D Systems Inc., Minneapolis, MN, USA; pro-BDNF—Aviscera Bioscience, Santa Clara, CA, USA). ELISAs were performed according to the manufacturer’s protocol. A total of 30 μg/μL of protein from each plasma sample was diluted 70-fold (MMP-9) or 20-fold (BDNF and pro-BDNF) with calibration diluent from the assays and analyzed in duplicate. The optical density of wells was determined at 450 nm using an automated microplate reader (Sunrise Microplate Absorbance Reader, Tecan, Männedorf, Switzerland).

Matusiak M., Oziębło D., Ołdak M., Rejmak E., Kaczmarek L, & Skarżyński H. (2023). Longitudinal Changes in BDNF and MMP-9 Protein Plasma Levels in Children after Cochlear Implantation. International Journal of Molecular Sciences, 24(4), 3714.

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