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Ki67 bv786

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Ki67-BV786 is a fluorochrome-conjugated antibody that binds to the Ki67 nuclear antigen, which is a cellular marker for proliferation. This product is intended for flow cytometry applications.

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Lab products found in correlation

Cd4 apcef780, by Thermo Fisher Scientific (1 mentions) Lsrfortessa analyzer, by BD (1 mentions) Cd45ro fitc, by Thermo Fisher Scientific (1 mentions) Cd45ra percp cy5, by BioLegend (1 mentions) Cd8 bv605, by BioLegend (1 mentions) Cd28 pe cy7, by BioLegend (1 mentions) Cd38 apc, by Thermo Fisher Scientific (1 mentions) Live dead fixable blue dead cell stain, by Thermo Fisher Scientific (1 mentions) Cd3 v500, by BD (1 mentions) Zombie aqua fixable viability dye, by BioLegend (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Cd69 apc, by BD (1 mentions) Ccr7 bv711, by BioLegend (1 mentions) Cd14 bv650, by BioLegend (1 mentions) Tnf bv605, by BioLegend (1 mentions) Cd28 ecd, by Beckman Coulter (1 mentions) Ki67 fitc, by BD (1 mentions) Cd8 bv570, by BioLegend (1 mentions) Cd20 bv650, by BioLegend (1 mentions) Cd95 pe cy5, by BD (1 mentions)

3 protocols using ki67 bv786

1

Phenotypic Analysis of PBMCs

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Peripheral mononuclear cells (PBMCs) were isolated through Ficoll-Paque density gradient. 2×106 PBMCs were stained for phenotypic analysis with the following fluorochrome-conjugated
antibodies: CD3-V500 (BD Biosciences), CD4-APCeF780 (eBiosciences), CD8-BV605 (Biolegend), CD28-PE-Cy7 (Biolegend), CD38-APC (eBiosciences), CD45RO-FITC (eBiosciences), HLA-DR-AF700 (eBiosciences), CD45RA-PerCP-Cy5.5 (Biolegend). Viability was determined with Live/Dead fixable blue dead cell stain (Invitrogen). Intracellular stain was performed with FoxP3-Pacific Blue (eBiosciences) and Ki-67-BV786 (BD Biosciences) following the eBiosciences Fixation/Permeabilization protocol (Invitrogen). Samples were analyzed on a BD LSRFortessa analyzer (BD Biosciences). Flow cytometry data analysis was done with FlowJo V10, LLC Software.
Castro-Rojas C.M., Godarova A., Shi T., Hummel S.A., Shields A., Tremblay S., Alloway R.R., Jordan M.B., Woodle E.S, & Hildeman D.A. (2020). mTOR Inhibitor Therapy Diminishes Circulating CD8+ CD28− Effector Memory T Cells and Improves Allograft Inflammation in Belatacept-Refractory Renal Allograft Rejection. Transplantation, 104(5), 1058-1069.
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2

Cell Proliferation Analysis by Flow Cytometry

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Cells were seeded at 0.3 × 106 cells/well in 6-well plates, incubated for 16 h, and analysed using the BD LSRFortessa X-20 (BD Biosciences, CA, USA). Anti-human Fc block (1:50, BD Biosciences, CA, USA) was used to block non-specific Fc receptor binding. Zombie Aqua™ fixable viability dye (BioLegend, CA, USA) was used to distinguish live and dead cells. Cells were stained with Ki67-BV786 (BD Biosciences, 1:100, clone #B56) for analysis of cellular proliferation and immediately analysed. Single stained cells were used to set compensation controls, and fluorescence-minus one (FMO) controls were used to define population gates. Data was acquired using a BD LSRFortessa X-20 (BD Biosciences, CA, USA), and was analysed using FlowJo software v10.5.0 (LCC, OR, USA).
Karimnia N., Wilson A.L., Green E., Matthews A., Jobling T.W., Plebanski M., Bilandzic M, & Stephens A.N. (2021). Chemoresistance is mediated by ovarian cancer leader cells in vitro. Journal of Experimental & Clinical Cancer Research : CR, 40, 276.
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3

Comprehensive Lymphocyte Immunophenotyping

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The following antibodies were used for phenotyping and functional evaluation of lymphocytes in this study: CD107a-BV421 (Clone H4A3, Biolegend), CD4-PECy5.5 (Clone S3.5, Invitrogen), CD8-BV570 (Clone RPA-T8, Biolegend), CD14-BV650 (Clone M5E2, Biolegend), CD20-BV650 (Clone 2H7, Biolegend), CD16-BV650 (Clone 3G8, Biolegend), CD28-ECD (Clone CD28.2, Beckman Coulter), CD95-PECy5 (Clone Dx2, BD Biosciences), CD38-PE (Clone OKT10, NIH NHP Reagent Resource, University of Massachusetts, Boston, MA), CCR7- BV711 (Clone G043H7, Biolegend). CD3-APCCy7 (Clone SP34-2, BD Biosciences), granzyme B-AF700 (Clone GB11, BD Biosciences), T-bet-PECy7 (Clone 4B10, eBioscience), Ki67-FITC (Clone B56, BD Bioscience) or Ki67-BV786 (Clone B56, BD Bioscience), CD69-APC (Clone FN50, BD Bioscience) or CD69-BV605 (Clone FN50, Biolegend), Perforin-FITC (Clone pf344, MabTech), TNF-BV605 (Clone MAb11, Biolegend), IFNg-BV785 (Clone 4S.B3, Biolegend), Live Dead Fixable Aqua Dead Cell Stain (Molecular Probes).
Roberts E.R., Carnathan D.G., Li H., Shaw G.M., Silvestri G, & Betts M.R. (2016). Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathogens, 12(12), e1006135.
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