Cup horn sonicator

Manufactured by Bioventus

Sourced in United States

The cup-horn sonicator is a laboratory instrument designed to disrupt cells and homogenize samples through the application of ultrasonic waves. It functions by placing the sample container within a specialized cup-shaped chamber, where the ultrasonic energy is directed to the sample, enabling efficient sample processing.

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Lab products found in correlation

Circulating water bath, by Bioventus (2 mentions) Mammalian chip on chip protocol, by Agilent Technologies (1 mentions) Limulus amoebocyte lysate assay, by Cambrex (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Sonicator (21 citations)

5 protocols using cup horn sonicator

Histone Acetylation Profiling in Mammalian Cells

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90-8TLs were treated with vehicle or vorinostat for 6 hours and then cross-linked for 10 minutes with an 11% formaldehyde solution, and cell lysis was performed according to the Agilent mammalian ChIP-on-Chip protocol. Lysates were sonicated on ice for 45 minutes, 20 seconds on, 40 seconds off, in a Misonix Cup Horn Sonicator at 4˚C. Chromatin was immunoprecipitated overnight at 4˚C with acetylated histone H4 antibody (Active Motif) or rabbit IgG (Millipore), which had first been conjugated to protein G magnetic beads (Life Technologies 10004D).

Malone C.F., Emerson C., Ingraham R., Barbosa W., Guerra S., Yoon H., Liu L.L., Michor F., Haigis M., Macleod K.F., Maertens O, & Cichowski K. (2017). mTOR and HDAC inhibitors converge on the TXNIP/thioredoxin pathway to cause catastrophic oxidative stress and regression of RAS-driven tumors. Cancer discovery, 7(12), 1450-1463.

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Silica Particle Preparation for Cell Assays

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Acid washed crystalline silica (Min-U-Sil-5, mean particle diameter 1.5-2 μm) was obtained from Pennsylvania Glass Sand Corp (Pittsburgh, PA, USA). Silica was determined to have insignificant levels of endotoxin (LPS) by the Limulus amoebocyte lysate assay (Cambrex, Walkersvill, MD, USA) as previously described (13 (link), 17 (link)). Prior to instillation into mice or addition to cell cultures, silica particles were suspended in PBS and sonicated for >1 min (550 watts @ 20 kHz) by a cup-horn sonicator in a circulating water bath (Misonix, Inc. Farmingdale, NY, USA).

Jessop F., Hamilton R.F., Rhoderick J.F., Shaw P.K, & Holian A. (2016). Autophagy Deficiency in Macrophages Enhances NLRP3 Inflammasome Activity and Chronic Lung Disease Following Silica Exposure. Toxicology and applied pharmacology, 309, 101-110.

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Preparation of Crystalline Silica Particles

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Crystalline silica (Min-U-Sil-5, average particle size 1.5-2 μm in diameter) was obtained from Pennsylvania Sand Glass Corporation (Pittsburgh, PA), and acid washed in 1N HCl. The cSiO₂ was washed with sterile water four times and dried in an oven at 200°C. Before use, cSiO₂ particles were suspended in PBS or dispersion media (for in vivo experiments) and sonicated for at least 2 min (550 watts at 20 kHz) by a cup-horn sonicator in a circulating water bath (Misonix, Inc. Farmingdale, NY). Dispersion media consisted of PBS containing 0.6 mg/ml mouse serum albumin and 0.01 mg/ml 1,2-dipalmitoyl-sn-glycero-3-phosphocholine.

Burmeister R., Rhoderick J.F, & Holian A. (2019). Prevention of Crystalline Silica-Induced Inflammation by the Anti-malarial Hydroxychloroquine. Inhalation toxicology, 31(7), 274-284.

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Optimized Enzymatic Digestion Protocol

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For standard conditions, enzymatic digestion of BR and Selenoprotein S (10 μg) was carried out with either trypsin or chymotrypsin in 50 mM ammonium bicarbonate (ABC, Fisher Scientific) buffer without any sonication. In the improved method, the same amount of model protein was sonicated on ice in 50 mM ABC containing 0%, 10% or 30% MeOH, without detergent, or with 0.01% RapiGest, 0.01% SDS, or 0.1% SDS. The sonication was performed with a cup horn sonicator (Misonix, Farmingdale, NY) set to 42 W output energy. Samples were subsequently subjected to reduction with 100 mM DTT for 25 min at 95 °C followed by alkylation with 150 mM iodoacetamide for 30 min at room temperature in the dark. Although BR does not contain disulfide bonds, the reduction step was incorporated to keep the protocol consistent. After a second sonication on ice for 5 min, enzymatic digestions were carried out with trypsin or chymotrypsin at 37 °C for 16 hours at an enzyme/substrate mass ratio of 1:20. The digest was then acidified with 10% formic acid (Acros Organics, Fair Lawn, NJ), and incubated at room temperature for 2 hours followed by centrifugation at 16000 g for 5 min.

Min L., Choe L.H, & Lee K.H. (2015). Improved protease digestion conditions for membrane protein detection. Electrophoresis, 36(15), 1690-1698.

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Corneocyte Stability Analysis in KCs

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Corneocyte stability was investigated as described previously (Fischer et al., 2014) . Briefly, KCs were seeded in six-well plates and transfected with siRNAs as described above. CNFN-deficient and control skin models were dissolved in 2% SDS, and the suspensions were sonicated using a cup horn sonicator (Misonix, Farmingdale, NY) at an amplitude of 50%, a power of 2 W, and cycles of 0.5 seconds. Every 30 seconds, aliquots were removed, and KCs were counted with a counting chamber under the microscope. The percentage of undamaged corneocytes at any time point were calculated relative to nonsonicated corneocyte counts. Criteria for intact KCs were shape, size, and reflection in the bright microscope. A representative example is shown in Supplementary Figure S6b.

Wagner T., Beer L., Gschwandtner M., Eckhart L., Kalinina P., Laggner M., Ellinger A., Gruber R., Kuchler U., Golabi B., Tschachler E, & Mildner M. (2019). The Differentiation-Associated Keratinocyte Protein Cornifelin Contributes to Cell-Cell Adhesion of Epidermal and Mucosal Keratinocytes. The Journal of investigative dermatology, 139(11).

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