Wet electrophoretic transfer unit

Manufactured by Bio-Rad

The Wet Electrophoretic Transfer Unit is a laboratory instrument used to transfer proteins or nucleic acids from a gel to a membrane for further analysis. It provides a controlled environment for the transfer process, ensuring efficient and consistent results.

Automatically generated - may contain errors

Lab products found in correlation

Mini protean tgx precast gel, by Bio-Rad (2 mentions) Polyvinylidene difluoride membrane, by Thermo Fisher Scientific (1 mentions) Mammalian protein extraction reagent buffer, by Thermo Fisher Scientific (1 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Cleaved caspase 3 no 9661, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Chemidoc imager, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions)

Close spelling variants of this product

Electrophoretic transfer unit Wet transfer unit Wet transfer Transfer unit

2 protocols using wet electrophoretic transfer unit

Fludarabine-Induced Protein Signaling in TSC2-null Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein lysis of TSC2-null 621-101 cells treated with Fludarabine was done using mammalian protein extraction reagent buffer (Thermo Fisher Scientific, no. 78501) containing protease and phosphatase inhibitors. Lysates were resolved on 4 to 20% Mini-PROTEAN TGX Precast gels (Bio-Rad, no. 4561094) and transferred using a wet electrophoretic transfer unit (Bio-Rad) onto polyvinylidene difluoride membranes (Thermo Fisher Scientific). After 1 hour of blocking with 5% nonfat dry milk, membranes were incubated with primary antibodies at 4°C overnight. Primary antibodies include: phospho-STAT1 (no. 8826), STAT1 (no. 14994), phospho-STAT3 (no. 9145), STAT3 (no. 30835), PBX1 (no. 4342), cleaved caspase-3 (no. 9661), PARP (no. 9532), cyclin D1 (no. 55506), phospho-RIPK1 (no. 44590), and β-actin (no. 3700) from Cell Signaling Technology. Secondary antibody incubation was done using horseradish peroxidase–linked antibodies for 2 hours. Signal was detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, no. PI34580).

Olatoke T., Wagner A., Astrinidis A., Zhang E.Y., Guo M., Zhang A.G., Mattam U., Kopras E.J., Gupta N., Smith E.P., Karbowniczek M., Markiewski M.M., Wikenheiser-Brokamp K.A., Whitsett J.A., McCormack F.X., Xu Y, & Yu J.J. (2023). Single-cell multiomic analysis identifies a HOX-PBX gene network regulating the survival of lymphangioleiomyomatosis cells. Science Advances, 9(19), eadf8549.

+ Open protocol

+ Expand

Endothelial Cell and Platelet Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blots were performed as previously described^{39 (link)}. In brief, endothelial cells or platelets were lysed with Laemmli sample buffer (Bio-Rad), boiled for 5 min at 95 °C, resolved on 4–20% Mini-PROTEAN TGX Precast gels (Bio-Rad), and transferred using a wet electrophoretic transfer unit (Bio-Rad) onto nitrocellulose membranes. After 1-h blocking with 5% nonfat milk in PBS containing 0.05% Tween 20 at room temperature, the membranes were hybridized with primary antibodies followed by HRP-conjugated secondary antibodies and enhanced chemiluminescence detection using a Bio-Rad Chemidoc Imager and ImageLab software (Bio-Rad) (see Supplementary Fig. 3).

Bennett J.A., Mastrangelo M.A., Ture S.K., Smith C.O., Loelius S.G., Berg R.A., Shi X., Burke R.M., Spinelli S.L., Cameron S.J., Carey T.E., Brookes P.S., Gerszten R.E., Sabater-Lleal M., de Vries P.S., Huffman J.E., Smith N.L., Morrell C.N, & Lowenstein C.J. (2020). The choline transporter Slc44a2 controls platelet activation and thrombosis by regulating mitochondrial function. Nature Communications, 11, 3479.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!