Biosciences. Anti-caspase 8 (52183, for immunofluorescence), anti-RIPK3 (56164),
and anti-adenine nucleotide translocase (ANT, 109864) antibodies were from
Abcam. Anti-CypD (AP1035) antibody was from Calbiochem. Anti-hexokinase II
(2106), anti-BAX (2772), anti-Bcl-xL (2762), anti-caspase 3 (9662),
anti-caspase 9 (9502), anti-BID (2002), anti-GAPDH (2118), and anti-caspase 8
(Asp387, 14071, for flowcytometry) were from Cell Signaling Technology.
Anti-VDAC (SP5361P) antibody was from Acris Antibodies. Active caspase 3 for
flowcytometry was from BD. Anti-human mitochondria antibody (HMS-0100) was from
Immunovision. Dylight 650-conjugated Donkey anti-rabbit IgG antibody and Alexa
fluro 488-conjugated Donkey anti-human IgG antibody were from Jackson Immuno
Research Laboratory. The selective caspase 8 inhibitor z-IETD-FMK (FMK007) was
from R&D Systems. Gammabind protein G sepharose (17-0885-01) was from GE
Health Care. Cyclosporin A (30024), Tiron (89460) and Protease Inhibitor
Cocktail (P2714) from Calbiochem and Phosphatase Inhibitor Cocktail 1 (P2850)
was from SIGMA-ALDRICH. Mitochondria/Cytosol Fractionation Kit (ThermoFisher
Scientific Mitochondrial Isolation kit #89874) and MitoTracker Red
CMXRos (M7512), MitoTracker Red CM-H2XRos (M7513), and LIVE/DEAD
Fixable Dead Cell Stain Kits (MP34955) from Invitrogen.