To determine the vesicle size of different ILEP formulations, a sample was diluted and investigated using dynamic light scattering on a piece of zeta sizer equipment (Malvern, Worcestershire, UK) [29 (link)].
Zetasizer equipment
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer is a laboratory instrument designed to measure the size and zeta potential of particles and molecules in a liquid sample. It uses the principles of dynamic light scattering and electrophoretic light scattering to determine these properties.
Automatically generated - may contain errors
10 protocols using zetasizer equipment
1
Measuring Vesicle Size in ILEP Formulations
2
Characterization of ATM-Loaded Solid Lipid Nanoparticles
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The average particle size diameter in (nm) was measured by Malvern Zetasizer equipment at 25 °C, backscatter detection of 173 °C, and refractive index of 1.330 (Malvern Instruments, Ltd., Malvern, UK). The formulations were diluted with deionized water in a ratio of (1:10), which provided suitable scattering intensity to measure the size of ATM-loaded SLNs. Dynamic light scattering (DLS) was used to measure the PDI of (ATM-SLNs). DLS detects vesicle distribution. The ZP was carried out in deionized water by detecting the electrophoretic mobility of the charged solid lipid nanoparticles in an electrical field, and that indicated their permeation behavior by studying their colloidal property and stability. PDI values less than 0.5 are more common to monodisperse samples, while values larger than 0.7 are common to a broad size (e.g., polydisperse) distribution of particles [57 (link),58 (link)]. The results are the mean values of three runs ± SD.
3
Glutathione-Loaded Multilayer Capsules
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Several capsule options were prepared using the layer-by-layer method (LbL). The first option was a two-layered capsule with varying glutathione contents of either 5 mg/mL H2O or 25 mg/mL H2O. The glutathione solutions prepared in this manner constituted the core of the capsules, on which the first layer of FUR was applied, and then the layer of CHIT, while mixing with a magnetic stirrer at 400 rpm. The capsules prepared in this way were named 2L5 and 2L25, respectively. GSH encapsulation was achieved by layering biopolymers (GSH/FUR/CHIT) at a ratio of 0.3/0.08/0.08. Another type of capsules was prepared by layering a glutathione core of 25 mg/mL first with a layer of negatively charged FUR, then positively charged CHIT, then FUR, and a final and fourth layer made of CHIT. These capsules were named 4L25. GSH encapsulation was achieved by layering biopolymers (GSH/FUR/CHIT/FUR/CHIT) at a ratio of 0.3/0.08/0.08/0.25/0.26. The preparation of the capsules was monitored using Malvern Zetasizer equipment, controlling the zeta potential.
4
Zeta Potential Measurement of Particles
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The zeta potential of the particles was measured by Zetasizer equipment (Malvern Panalytical Ltd., Malvern, UK). Samples were diluted 5 times using 10 mM phosphate buffer, pH 7.4. For each sample, the measurement was repeated 3 times.
5
Particle Size and Zeta Potential Analysis
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All of the samples (Col-AgNPs and AgNPs) were diluted with ultra-purified water at a concentration of 200 μg/mL. The particle size analysis of samples was performed by DLS using Zetasizer equipment at 25 °C (Malvern Panalytical Ltd., Malvern, UK). The particle size of all of the samples was performed at an angle of 90. The zeta potential of the samples was determined by electrophoretic mobility using Zetasizer at 25 °C, and the measurements were performed in triplicate.
6
Characterization of Inclusion Complex
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The particle sizes and zeta potentials of β-CD-PCL-AD/β-CD-PCL-b-PDMAEMA inclusion complex with enclosing plasmid DNA (pDNA) were measured using Malvern Zetasizer equipment at 25 °C. The data of the particle size and zeta potential were collected with detection angles of scattered light at 90° and 15°, respectively, and the dispersant was set as phosphate-buffered saline (PBS) buffer.
7
Characterization of PAC-IONs: A Comprehensive Evaluation
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Morphological, physical and chemical characteristics of the PAC-IONs were evaluated by different methods. The hydrodynamic particle diameter, size distribution and zeta potential were measured by dynamic light scattering with Zetasizer equipment (Malvern Panalytical, Almelo, The Netherlands) at room temperature. To this purpose, dried PAC-IONs were resuspended (1 mg/10 ml) in a 50:50 (v/v) ethanol–water mixture for triplicate-run analysis of size distribution. Another aliquot of dried PAC-IONs was resuspended in water (0.5 mg/10 ml) to evaluate the zeta potential using the Smoluchowski equation. Additionally, the ethanol–water suspension was also utilized for analyzing the particle morphology by transmission electron microscopy, using a JEOL 100-CX II microscope (Jeol Ltd, Tokyo, Japan).
The magnetization of the PAC-IONs was determined in a sample of dried particles under an applied MF with a Physical Property Measurement System (Quantum Design, CA, USA), using a Vibrating Sample Magnetometer and -0.5 to 0.5 T scan at room temperature (300°K). A summary of the physical properties of the PAC-IONs is included inTable 1 .
The magnetization of the PAC-IONs was determined in a sample of dried particles under an applied MF with a Physical Property Measurement System (Quantum Design, CA, USA), using a Vibrating Sample Magnetometer and -0.5 to 0.5 T scan at room temperature (300°K). A summary of the physical properties of the PAC-IONs is included in
8
Characterizing C@Ag-NPs Hydrodynamic Properties
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The hydrodynamic diameter and potential charge of the C@Ag-NPs suspensions were detected using zeta sizer equipment (Malvern, UK). Briefly, C@Ag-NPs suspensions were tenfold diluted using dist. H2O, sonicated for 15 min, and then transferred into U-type tubes at 25 °C for measurement using a zeta sizer.
9
Characterization of ZnO2 Nanoparticles
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The morphological characteristics of the NPs were observed using an FEI Talos L120C transmission electron microscope (120 kV). The X-ray diffraction (XRD) patterns of the ZnO2 NPs were measured on a Rigaku D/MAX-2250 V device. The zeta potentials of the ZnO2, ZnC and ZnCM NPs were measured with Malvern Zetasizer equipment.
10
Characterization of MnO2/Ce6 Liposomes
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The morphology and particle size of MnO 2 /Ce6@Liposomes were observed using transmission electron microscopy (TEM, JEM-1400plus, Japan). The morphology of MnO 2 /Ce6@MBs was investigated using an FL microscope (80I, Japan). The particle size distribution and zeta potential of MnO 2 /Ce6@MBs were measured by dynamic light scattering (DLS) using a zetasizer equipment (Malvern Instruments, UK). The ultraviolet absorption spectrum was detected by using an ultravioletvisible (UV-Vis) spectrometer (UV-1200, Shanghai Mipuda). The concentration of MnO 2 /Ce6@MBs was detected by using a particle counter (PSS, 780-A 7000APS, USA).
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