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Na ionophore 1 eth 227

Manufactured by Merck Group
Sourced in United States

Na+ Ionophore I-ETH 227 is a laboratory reagent used for the selective detection and quantification of sodium ions (Na+) in various sample types. It functions as an ionophore, a compound that facilitates the transport of specific ions across cell membranes or other barriers. The core function of Na+ Ionophore I-ETH 227 is to enable the measurement of sodium ion concentrations in research and analytical applications.

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2 protocols using na ionophore 1 eth 227

1

Calibrating Ion-Selective Microelectrodes

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Double/barreled Ca2+-selective and Na+-selective microelectrodes were prepared as described previously [26] (link). The Ca2+ ionophore II-ETH 129 or the Na+ Ionophore I-ETH 227 (both Fluka Sigma-Aldrich, MO, USA) were used to back-fill the Ca2+ and Na+-selective microelectrodes, respectively. Each ion-selective microelectrode was individually calibrated as described previously [26] (link). After making measurements of intracellular resting Ca2+ ([Ca2+]i) and resting Na+ concentration ([Na+]i) all microelectrodes were recalibrated, and if the two calibration curves did not agree within 3 mV, data from that microelectrode were discarded.
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2

Selective Inhibition of IP3 Receptors

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Xestospongin C, a selective IP3R blocker, was obtained from Cayman Chemical Company (MI, USA). Gadolinium chloride, Nifedipine, Ca2+ ionophore II – ETH 129, Na+- ionophore I - ETH-227 and the phospholipase C (PLC) inhibitor U-73122 were from Fluka Sigma-Aldrich (USA). All other chemicals were of the highest purity commercially available.
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