Nylon syringe membranes

Analysis of the LC-MS carried out, as described in Drouet et al. [37 (link)], on a Water 2695 Alliance coupled with a single quadrupole mass spectrometer ZQ (Waters-Micromass, Manchester, UK). LC-ESI-MS (liquid chromatography-electrospray ionization-mass spectrometry) MassLynx 4.0 software (Waters-Micromass, Manchester, UK) was used to acquire and process data. The separation was conducted using a linear gradient: from a mixture of 10:90 (v/v) to 100:0 (v/v) of solvent A (methanol) and solvent B (water + 0.05% (v/v) formic acid) at a flow rate of 1.00 mL/min during a run of 1 h. The detection was set at 350 nm. Before injection, extracts were centrifuged 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France) and the supernatant extract was filtered through 0.45 μm of nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France).

Sample (100 mg) was suspended in 1 mL aqEtOH 90% (v/v) in 5 mL quartz tubes equipped with a vapor condenser. Extraction was performed at 45 °C in a water bath under agitation (150 rpm) for 46 min. After extraction, the extract was centrifuged 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France) and the supernatant extract was filtered through 0.45 μm of nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France).

Sample (100 mg) was suspended in 1 mL 90% (v/v) aqEtOH in 5 mL quartz tubes equipped with a vapor condenser. Extraction was performed at 45 °C in USC1200TH (Prolabo, Fontenay-sous-Bois, France) ultrasonic bath operating at a 30 kHz frequency for 46 min. After extraction, the extract was centrifuged 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France) and the supernatant extract was filtered through 0.45 μm of nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France).