Heparinases 1 and 3

The following materials were purchased from the manufacturers indicated: heparin, heparan sulfate, chondroitin sulfate A, B and C, heparinases I and III, chondroitinase ABC, fluorescein isothiocyanate (FITC), GenElute PCR clean-up kit, phospholipase C phosphatidylinositol-specific (PI-PLC) from Bacillus cereus, all from Sigma-Aldrich (St. Louis, MO, USA); 2-O, 6-O and N-desulfated heparins from Amsbio (Abingdon, UK); Dulbecco’s Modified Eagle’s minimal essential medium (DMEM) and Minimum Essential Medium (MEM), fetal bovine serum, penicillin-streptomycin, and PBS-phosphate-buffered saline from Gibco-Thermo Fischer Scientific (Waltham, MA, USA); Brain-Heart Infusion broth from Pronadisa (Madrid, Spain); RNeasy Kit and RNase-Free DNase from Qiagen (Hilden, Germany); High-Capacity cDNA Reverse Transcription Kit and PowerSYBR Green PCR Master Mix from Applied Biosystems (Foster City, CA, USA). Synthetic peptides were from Abyntek Biopharma (Derio, Spain); mouse monoclonal anti-syndecan 1 (CD138) from DakoCytomation (Carpinteria, CA, USA); and rabbit anti-syndecan 2, goat anti-syndecan 3 and rabbit anti-syndecan 4 polyclonal antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All other chemicals were obtained from commercial sources and were of analytical grade.

The following materials were purchased from the manufacturers indicated: HS, chondroitin sulfates A, B, and C (CS A, CS B, and CS C), Heparinases I and III, chondroitinase ABC, fluorescein isothiocyanate (FITC), and phospholipase C phosphatidylinositol-specific (PI-PLC) from Bacillus cereus, all from Sigma-Aldrich (St. Louis, MO, USA); 90% DMEM/F-12 + GlutaMAX™ [Dulbecco's Modified Eagle's medium F-12 Nutrient Mixture (Ham)], fetal bovine serum, penicillin-streptomycin, insulin, EGF (epidermal growth factor recombinant protein), and PBS (phosphate-buffered saline), from Gibco-Thermo Fischer Scientific (Waltham, MA, USA); HiTrap Desalting column and superose 12 from GE Healthcare Life Sciences (Little Chalfont, UK); Brain-Heart Infusion broth (BHI), from Pronadisa (Madrid, Spain); RNeasy Kit and RNase-Free DNase, from Qiagen (Hilden, Germany); High-Capacity cDNA Reverse Transcription Kit and PowerSYBR Green PCR Master Mix, from Applied Biosystems (Foster City, CA, USA); synthetic peptides were from Abyntek Biopharma (Derio, Spain); mouse monoclonal anti-syndecan 1 (CD138), from DakoCytomation (Carpinteria, CA, USA); and rabbit anti-syndecan 2, goat anti-syndecan 3, and rabbit anti-syndecan 4 polyclonal antibodies, from Santa Cruz Biotechnology (Santa Cruz, CA, USA).