Equinox 55 s ft ir spectrophotometer

All manipulations were performed under aerobic conditions using materials as received (Aldrich Co). All chemicals and solvents were of reagent grade. The ligand OH-C₆H₄-CH=NC(CH₂OH)₃, H₄L was synthesized as described previously [57 (link)]. Elemental analysis for carbon, hydrogen, and nitrogen was performed on a Perkin Elmer 2400/II automatic analyzer. Infrared spectra were recorded as KBr pellets in the range 4000–400 cm⁻¹ on a Bruker Equinox 55/S FT-IR spectrophotometer. Variable-temperature magnetic susceptibility measurements were carried out on polycrystalline samples of 1–4 by using a SQUID magnetometer (Quantum Design MPMS 5.5). Diamagnetic corrections were estimated from Pascal′s constants. The program PHI was used for simulations of the magnetic susceptibility data of 3 [58 (link)].

UV spectra were recorded on an Aminco/OLIS UV-Vis spectrophotometer. IR spectra were measured with a Bruker Equinox 55/S FT–IR spectrophotometer. NMR spectra were obtained in CD₃OD (δ_H 3.34 ppm, δ_C 49.0 ppm) with a Bruker Avance 600 III MHz (Billerica, MA, USA) spectrometer equipped with a ¹H{¹³C/¹⁵N/³¹P} cryoprobe, a Bruker Avance III 500 MHz (Billerica, MA, USA) spectrometer equipped with a ¹³C/¹⁵N{¹H} cryoprobe, and a Bruker Avance III HD 400 MHz (Billerica, MA, USA) spectrometer. HRMS data were acquired with a Bruker MaXis™ 4G ESI-QTOF (Billerica, MA, USA) mass spectrometer. RP HPLC was performed using a Shimadzu Prominence HPLC system and a Phenomenex Gemini C18 column (250 × 30 mm). UHPLC-HRMS was acquired using a Bruker MaXis™ 4G ESI-QTOF (Billerica, MA, USA) mass spectrometer coupled with a Waters Acquity UPLC system operated by Bruker Hystar software and a C18 column (Phenomenex Kinetex 2.6 μm, 2.1 mm × 100 mm). Bruker timsTOF Pro instrument (Billerica, MA, USA) was used for the trapped ion mobility MS analysis using direct infusion with 0.003 mL/min of flow rate and ESI+ ionization source. Nebulizer gas 0.4 bar, dry gas 3.5 L/min, source temperature 220 °C, ESI voltage 4200V (+). MS spectra were collected using the following parameters: tims ramp time = 350 ms, PASEF on, scan range (m/z, 20–1000; 1/k₀, 0.70–1.00 V·s/cm².