For immunostaining, 7 µm tissue sections of the spleens were stained using PE-conjugated anti-IL-1R, PE-conjugated anti-p-STAT3 (Y705), PE-conjugated anti-p-STAT3 (S727), PE-conjugated anti-IL-1R, PE-conjugated anti-IL-1β, FITC-conjugated anti-Foxp3, PE-conjugated anti-IL-17, APC-conjugated anti-CD25, FITC conjugated anti-CD4 (all from eBioscience, San Diego, CA) and with DAPI (Sigma) overnight at 4°C. The stained sections were analyzed using a Zeiss microscope (LSM 510 Meta; Carl Zeiss, Oberkochen, Germany) at 3400 magnification.
Yang E.J., Lee J., Lee S.Y., Kim E.K., Moon Y.M., Jung Y.O., Park S.H, & Cho M.L. (2014). EGCG Attenuates Autoimmune Arthritis by Inhibition of STAT3 and HIF-1α with Th17/Treg Control. PLoS ONE, 9(2), e86062.
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