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Anti hcd45ro pecy7

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Anti-hCD45RO-PECY7 is a fluorescently labeled monoclonal antibody that binds to the CD45RO isoform of the human CD45 protein. CD45RO is a marker of memory T cells. This antibody can be used for the identification and analysis of memory T cells in flow cytometry applications.

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Lab products found in correlation

Live dead fixable violet dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Software version 7, by FlowJo (1 mentions) Facsaria iiu, by BD (1 mentions) Goat anti human igg pe, by Southern Biotech (1 mentions) Lsrii system, by BD (1 mentions) Anti cd69 pe, by BioLegend (1 mentions) Facscalibur, by BD (1 mentions) Streptavidin pe, by BD (1 mentions)

2 protocols using anti hcd45ro pecy7

1

Multiparametric Flow Cytometry Phenotyping

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After a 6-hr culture, the cell populations were stained with a live/dead fixable violet dead cell stain kit (Invitrogen, Carlsbad, CA, USA) for distinction of dead cells according to the manufacturer's instructions, and the following monoclonal antibodies were used: anti-hCD3-V500, anti-hCD4-PerCP, anti-hCD8-Alexa 700, anti-hCD69-APCCy-7, anti-hCD45RO-PECY7, or CD45RA-PECY7 / anti-hCD62L (BioLegend, San Diego, CA and BD biosciences San Jose, CA, EUA). Flow cytometric analysis was performed using a FACSAria IIu flow cytometer (BD Biosciences) and the FlowJo software version 7.5 (FlowJo LLC, Ashland, OR, USA).
de Carvalho F.M., Rodrigues L.S., Duppre N.C., Alvim I.M., Ribeiro-Alves M., Pinheiro R.O., Sarno E.N., Pessolani M.C, & Pereira G.M. (2017). Interruption of persistent exposure to leprosy combined or not with recent BCG vaccination enhances the response to Mycobacterium leprae specific antigens. PLoS Neglected Tropical Diseases, 11(5), e0005560.
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2

Multiparametric Flow Cytometry Analysis

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Anti-hCD25-PE, anti-CD69-PE, anti-hCD8-APC, anti-hCD45-PECy7, anti-hCD4-PE, anti-hCD4-BV421, anti-hCD62L-PercpCy5.5, anti-hPD1-BV421 and anti-hCD45RO-PECy7, were from Biolegend. Goat anti-human IgG-PE was from SouthernBiotech. Streptavidin-PE, anti-hCD4-APC and anti-hCD25-APC were from BD biosciences. All FACS analyses were carried out using FACSCalibur or LSRII system (BD Biosciences) and analyzed using FlowJo (FLOWJO).
Wu Z., Guo H.F., Xu H, & Cheung N.K. (2018). Development of a tetravalent anti-GPA33/anti-CD3 bispecific antibody for colorectal cancers. Molecular cancer therapeutics, 17(10), 2164-2175.
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