Cml elisa kit

CML was purchased from PolyPeptide Laboratories (San Diego, USA). Oxidized low-density lipoprotein (oxLDL) was acquired from Yiyuan Biotechnology (Guangzhou, China). The CML ELISA kit was provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). RAGE and galectin-3 siRNA were purchased from RiboBio Biotechnology (Guangzhou, China), smooth muscle (SM) antiactin was purchased from Sigma-Aldrich (St. Louis, MO, USA), and antibodies targeting RAGE, galectin-3, β-actin, and CD68 were obtained from Abcam (USA).

CML was purchased from PolyPeptide Laboratories (San Diego, USA). Oxidized low density lipoprotein (oxLDL) was acquired from Yiyuan Biotechnology (Guangzhou, China). RAW264.7 macrophages and A7r5 aortic vascular smooth muscle cells (VSMC) were obtained from ATCC (USA). CML ELISA kit, calcium assay, and ALP activity kits were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). von Kossa staining kit was acquired from Shunbai Biologicals Inc. (Shanghai, China). SB203580 (p38MAPK inhibitor) and DPI (NADPH oxidase inhibitor) were obtained from Sigma-Aldrich Co. LLC (USA). Anti-Nε-carboxymethyl-Lysine antibody was provided by Abcam (UK). The antibodies against RAGE, NADPH oxidase 4 (Nox4), cbfα1, P-p38MAPK, ALP, β-actin, and all secondary antibodies were provided by Santa Cruz (USA). All other chemicals and reagents were of analytical grade.