Cy3 conjugated goat anti rabbit igg

In order to further verify the specific location of SETD2 and H3K36me3 distribution in cells, microscopic confocal observation was used. The third generation of BDEs was chosen as the experimental subject. A total of 1×10⁵ cells/ml cell suspension was prepared from DMEM/F12 medium containing 10% calf serum, and inoculated in confocal culture dishes with clean sterile cover slides, and cultured in a cell incubator at 37°C with 5% CO₂ overnight. The slides were washed twice with PBS and 2 µl rabbit anti-mouse SETD2 (ABclonal Biotech Co., Ltd.; cat. no. A11757) and rabbit anti-H3K36me3 (ABclonal Biotech Co., Ltd.; cat. no. A2366) were added and incubated at room temperature for 40 min. The slides were washed twice with PBS and then 4 µl Cy3-conjugated goat anti-rabbit IgG (ABclonal Biotech Co., Ltd.; cat. no. AS007) was added at room temperature for 30 min. The slides were washed with PBS three times, before adding 2 µl DAPI dye solution for 5 min and washing with PBS another three times. The slides were sealed with a drop of 90% glycerin. An upper confocal microscope (Leica Microsystems, Inc.) was used to view the images and scan them using the Leica TCS SP8X system (Leica Microsystems, Inc.).