Antibodies used for multispectral imaging analysis are detailed in Key resources table . Staining of murine and human melanoma for multispectral imaging has previously been described (Mauldin et al., 2020 ). In brief, 4-μm thick sections were cut from formalin fixed paraffin embedded murine tumor specimens or human melanoma biopsies, and murine spleen and human lymph node biopsies was used as a positive control. Antigen retrieval was performed using AR9 buffer (PerkinElmer) according to the manufacturer instructions. OPAL Multiplex IHC Staining (PerkinElmer) was performed according to the manufacturer instructions. Slides were mounted using prolong diamond antifade (Life Technologies) and scanned at low magnification using the Vectra 3.0 system and software (PerkinElmer). Regions of interest were identified in Phenochart software, and high-powered magnification images were acquired with the Vectra 3.0 system (Akoya Biosciences). These images were spectrally unmixed using single stain positive controls and analyzed using the InForm software (PerkinElmer). Human specimen images were quantified using HALO software (Indica Labs, Albuquerque, NM) and murine specimens were quantified by eye.
Opal multiplex ihc staining
Manufactured by PerkinElmer
The OPAL Multiplex IHC Staining is a lab equipment product by PerkinElmer. It is designed for the simultaneous detection of multiple protein markers in a single tissue section using immunohistochemistry (IHC) techniques.
Automatically generated - may contain errors
Lab products found in correlation
Inform software, by PerkinElmer (2 mentions)
Prolong diamond antifade, by Thermo Fisher Scientific (2 mentions)
Vectra 3.0 system and software, by PerkinElmer (2 mentions)
Vectra 3.0 system, by Akoya Biosciences (2 mentions)
Ar9 buffer, by PerkinElmer (2 mentions)
Halo software, by Indica Labs (2 mentions)
2 protocols using opal multiplex ihc staining
1
Multispectral Imaging of Melanoma
2
Multispectral Imaging of Melanoma
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Antibodies used for multispectral imaging analysis are detailed in Key resources table . Staining of murine and human melanoma for multispectral imaging has previously been described (Mauldin et al., 2020 ). In brief, 4-μm thick sections were cut from formalin fixed paraffin embedded murine tumor specimens or human melanoma biopsies, and murine spleen and human lymph node biopsies was used as a positive control. Antigen retrieval was performed using AR9 buffer (PerkinElmer) according to the manufacturer instructions. OPAL Multiplex IHC Staining (PerkinElmer) was performed according to the manufacturer instructions. Slides were mounted using prolong diamond antifade (Life Technologies) and scanned at low magnification using the Vectra 3.0 system and software (PerkinElmer). Regions of interest were identified in Phenochart software, and high-powered magnification images were acquired with the Vectra 3.0 system (Akoya Biosciences). These images were spectrally unmixed using single stain positive controls and analyzed using the InForm software (PerkinElmer). Human specimen images were quantified using HALO software (Indica Labs, Albuquerque, NM) and murine specimens were quantified by eye.
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