Rebel t2i

Live material was studied using stereo and compound light microscopes Microscopes used were: optical Leitz Orthoplan 2 microscope with Canon Rebel T2i digital camera (Fig. 2a, c, e, f); differential interference contrast Leitz dialux 20 microscope with DS-Fi1 Nikon digital camera driven by the Nikon NIS-F v.4.0 software (Fig. 2b); optical Nikon Eclipse microscope with Cool Pix 4300 Nikon digital camera; optical Zeiss Axiostar microscope with Power Shot G6 Canon digital camera (Fig. 2d, g); differential interference contrast Nikon Eclipse 80i microscope with Canon EOS 5D mk III digital camera (Fig. 2h). Individuals from each identified morphotype (maximum 20 individuals per morphotype) were preserved in ethanol 90% for further molecular analyses. The taxonomic effort was focused on species belonging to the phyla Annelida, Gastrotricha, Mollusca, Nematoda, Nemertea, Platyhelminthes, and Xenacoelomorpha (Fig. 2). We followed the recent phylogenetic hypothesis and consensus that sipunculans are annelids, and include results from both taxa together in the phylum Annelida^{40 (link)}.

High resolution photographs of the twenty-one real objects were taken using a Canon Rebel T2i DSLR camera with constant F-stop and shutter speed. The photos were edited to remove background and resized to fit within a 600 × 600 pixel square. Thus, similar to previous behavioral and fMRI studies investigating object processing (e.g.^{24 (link),26 (link),31 (link),35 (link)}, the 2-D images had the same physical size on the screen (Fig. 1C). The 2-D images were displayed on a 27 inch ACER G27HL LCD monitor from a Dell Latitude E6430 computer and Logitech K120 keyboard and mouse.