V plex human biomarkers

Interleukin-10 (IL-10), interleukin-8 (IL-8), and transforming growth factor-beta isoform (TGF-β1, TGF-β2, TGF-β3) concentrations were measured in the conditioned media of cell and bacteria coculture under anaerobic conditions at 1, 6 and 24 h. IL-8 and IL-10 were analyzed using commercially available V-PLEX human biomarkers (Meso Scale Diagnostics, Rockville, MD, USA) multiplex assay. TGF-β1, TGF-β2, and TGF- β3 were analyzed using the multiplex assays (U-PLEX) for biomarker group 2 (Meso Scale Diagnostics).

The concentrations of inflammatory proteins in saliva samples were analyzed from the last six healthy control subjects from the original group of 16 controls with the purpose to investigate the possibility to detect these proteins in the samples. The samples were analyzed using a commercially available V-PLEX human biomarkers (Meso Scale Diagnostics, Rockville, MD, USA) multiplex assay. The assay contained 39 biomarkers (see Table 4) and was based on an electrochemiluminescent detection method and performed exactly according to the manufacturer’s recommendations. Data were collected and analyzed using MESO QUICKPLEX SQ 120 instrument equipped with DISCOVERY WORKBENCH^® data analysis software (Meso Scale Diagnostics, Rockville, MD, USA).
The precisions based on both intra- and interassay coefficient of variations were <10% within the detection limits. The detection limit for each investigated biomarker is shown in Table 4 [24 (link),25 (link)].
Total protein concentration was measured using 2D Quant-kit (GE Healthcare Biosciences AB, Uppsala, Sweden), according to the manufacturer’s protocol.