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Accel sirnas

Manufactured by Horizon Discovery
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Accel siRNAs are small interfering RNAs designed for efficient gene silencing. They are optimized for enhanced potency and specificity, enabling effective knockdown of target genes.

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Lab products found in correlation

Chemidoc imaging system, by Bio-Rad (2 mentions) Biolux gaussia luciferase assay kit, by New England Biolabs (2 mentions) Ab23750, by Abcam (2 mentions) Gaussia luciferase, by GeneCopoeia (2 mentions) Hpa002926, by Merck Group (2 mentions)

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SiRNAs (161 citations)

3 protocols using accel sirnas

1

Actin-Binding Proteins Targeted Silencing

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A total of 96 Accel siRNAs targeting actin-binding proteins (including control) were made to order (Dharmacon). Lists of targets and accession numbers are in the supplementary materials (supplemental Table 4).
El-Mansi S., Robinson C.L., Kostelnik K.B., McCormack J.J., Mitchell T.P., Lobato-Márquez D., Rajeeve V., Cutillas P., Cutler D.F., Mostowy S, & Nightingale T.D. (2022). Proximity proteomics identifies septins and PAK2 as decisive regulators of actomyosin-mediated expulsion of von Willebrand factor. Blood, 141(8), 930-944.
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2

Evaluating KLF4 Regulation of Gaussia Luciferase

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A KLF4 promoter-reporter plasmid that expresses the secretable Gaussia Luciferase was purchased from Genecopoeia, and transfected into plated MOVAS cells. 24 hours post-transfection, siRNAs targeting either KLF4 or non-target control were added to MOVAS cells according to manufacturer’s protocol (Dharmacon Accel siRNAs). 72 hours post-siRNA treatment, media was replaced with serum-free media containing recombinant proteins or tumor conditioned media. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). Reporter plasmid activity is reported in luminescence as compared to empty plasmid control. Western Blots were also performed after TCM treatment of KLF4 knocked down vSMCs to probe for KLF4 (HPA002926, Sigma) and fibronectin (ab23750, Abcam), and were imaged using the BioRad ChemiDoc imaging system.
Murgai M., Ju W., Eason M., Kline J., Beury D., Kaczanowska S., Miettinen M.M., Kruhlak M., Lei H., Shern J.F., Cherepanova O.A., Owens G.K, & Kaplan R.N. (2017). KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nature medicine, 23(10), 1176-1190.
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3

Evaluating KLF4 Regulation of Gaussia Luciferase

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A KLF4 promoter-reporter plasmid that expresses the secretable Gaussia Luciferase was purchased from Genecopoeia, and transfected into plated MOVAS cells. 24 hours post-transfection, siRNAs targeting either KLF4 or non-target control were added to MOVAS cells according to manufacturer’s protocol (Dharmacon Accel siRNAs). 72 hours post-siRNA treatment, media was replaced with serum-free media containing recombinant proteins or tumor conditioned media. 16 hours after protein-containing treatment, media was removed and assayed for luciferase activity using the BioLux Gaussia Luciferase Assay Kit (New England BioLabs). Reporter plasmid activity is reported in luminescence as compared to empty plasmid control. Western Blots were also performed after TCM treatment of KLF4 knocked down vSMCs to probe for KLF4 (HPA002926, Sigma) and fibronectin (ab23750, Abcam), and were imaged using the BioRad ChemiDoc imaging system.
Murgai M., Ju W., Eason M., Kline J., Beury D., Kaczanowska S., Miettinen M.M., Kruhlak M., Lei H., Shern J.F., Cherepanova O.A., Owens G.K, & Kaplan R.N. (2017). KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis. Nature medicine, 23(10), 1176-1190.
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