Clarus 600 c

The amount of rhynchophorol recovered was determine using a gas chromatograph (Clarus 680), coupled to a mass spectrometer detector (Clarus 600C), with an ELITE-5MS capillary column (Perkin Elmer/USA). Samples (1 µL) were injected through a CTC Combipal automatic injector (Pal System/Switzerland). The run conditions were: helium carrier gas with 1 mL min⁻¹ flow, 50 mL split, and injector temperature of 150 °C. The initial temperature of the oven was 50 °C for 3 min with a heat ramp of 10 °C min⁻¹ up to 200 °C, held for 1 min. The mass spectrometry detector was configured to operate with ionization of 70 eV in scanning mode (SCAN), in the mass range of 25–500 m/z. The temperatures were fixed at 200 °C for the ionization source and 180 °C for the quadrupole. The interface with the mass detector was kept at 200 °C.

The fatty acid composition of mango (M. indica and M. sylvatica) and cocoa butter (Deodorized and non-Deodorised) samples were done by GC (PerkinElmer Clarus 680)-MS (PerkinElmer Clarus 600 C). Five to seven mg of frozen butter dissolved in 1 ml of heptane and 0.05 ml of 1 N Methanolic NaOH were shaken at room temperature for two minutes. After 2 minutes when the two layers were separated the lower layer was discarded and the supernatant) used for GC-MS analysis. The analysis was done in triplicate. On the other hand, a direct infusion mass spectrometry method (API 150 EX MS System) used for the determination of Triglycerides. The nebulizer gas was N₂. Scanning done for mass 100 to 1000 in an ESI Positive mode with a flow rate of 90 μl/min. Samples were run once for triglyceride profiling2 7 (link) and percentage of triglycerides were calculated based on the peak intensity.

The GC–MS analysis of OSEO was carried out on PerkinElmer Clarus 600 C (Waltham, MA, USA) analytical system equipped with DB-5MS (30 m × 0.25 mm; 0.25 μm film thickness) merged silica capillary column and attached to flame-ionization detector (FID). OSEO was diluted in acetone (10 μL/mL) and 1 μL solution was injected in a split-mode (1:30). Working conditions were as follows: carrier gas was He (1 mL/min); temperatures were set as follows: injector at 250°C and sensor at 280°C, whilst the column temperature was linearly scheduled 40–280°C at 4°C/min. Mass spectra were documented in EI mode (70 eV) with a range of m/z 40–450. Turbo Mass software application was adapted to operate and acquire data from the GC–MS. The detection of the individual components was achieved by comparison of their mass spectra (MS) with those from accessible libraries (NIST/Wiley) and experimentally determined retention indices (RI) with data from the literature (Adams, 2007 ). The percentage of the constitution of the individual components was derived out from the peak areas, devoid of correction factors.

The catalytic tests were performed under MW irradiation in a focused microwave Anton Paar Monowave 300 discover reactor (5–10 W) using a 10 mL capacity reaction tube with a 13 mm internal diameter, fitted with a rotational system and an IR (Infrared) temperature detector. Gas chromatography (GC) measurements were carried out using a FISONS Instruments GC 8000 series gas chromatograph with a DB-624 (J&W) capillary column (flame ionization detector or FID detector) and the Jasco-Borwin v.1.50 software. The temperature of injection was 240 ˚C. The initial temperature was maintained at 120 ˚C for 1 min then raised 10 ˚C/min to 200 ˚C and held at this temperature for 1 min. Helium was used as the carrier gas. GC-MS analyses were performed using a Perkin-Elmer Clarus 600 C instrument (He as the carrier gas). The ionization voltage was 70 eV. Gas chromatography was conducted in the temperature-programming mode using a SGE BPX5 column (30 m × 0.25 mm × 0.25 μm). Reaction products were identified by comparison of their retention times with known reference compounds and by comparing their mass spectra to fragmentation patterns obtained from the NIST spectral library stored in the computer software of the mass spectrometer.

The analysis of FAMEs (Figure 2) was carried out on a “Clarus 600” system PerkinElmer, Inc. (Waltham, MA, USA) equipped with a quadrupole analyzer “Clarus 600 C” mass-selective detector (Waltham, MA, USA). The conditions were adopted from Radenkovs et al. [22 (link)].

The chemical constituent of CMEO was revealed using GC-MS (PerkinElmer Clarus 600 C, Waltham, United States) attached with DB-5MS silica fused column (30 m × 0.25 mm; 0.25 μm film thickness), flame-ionization detector (FID), and Turbo Mass software program. Briefly, CMEO was diluted with acetone (10 μL/mL) and 1 μL was injected in a split mode of 1:30. Helium (He) was carrier gas at a delivery rate of 1 mL/min. The mass spectra of compounds were determined at an m/z range of 40–450 and ionization energy of 70 eV under vacuum. Identification of the compounds was carried out matching the mass spectra and retention indices of n-alkanes (C-9 to C-24) with NIST05.LIB/Wiley 8th Edition and literature of Adams (2007) , respectively. The quantification of compounds was attained from GC peak areas with devoid of FID response correction factors.