Methanol of lc ms grade

Manufactured by Merck Group

Sourced in United States, Germany

Methanol of LC-MS grade is a high-purity solvent used in liquid chromatography-mass spectrometry (LC-MS) applications. It serves as a mobile phase component for the separation and analysis of various chemical compounds. This solvent meets the stringent purity requirements necessary for sensitive LC-MS instrumentation.

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LC-MS grade methanol LC-MS/MS grade methanol LC-grade methanol MS-grade methanol Methanol LC–MS LC-MS grade Methanol

7 protocols using methanol of lc ms grade

Quantification of Ligandrol (LGD-4033) and Metabolites

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The standard of ligandrol (LGD-4033) was purchased from Toronto Research Chemicals; TRC (Toronto, ON, Canada). Stock solution of standard substance was prepared at the concentration of 1 mg/mL and its working solutions (100 µg/mL, 10 µg/mL, and 1 µg/mL) in methanol and stored at −20 °C.
Due to unavailability of certified reference materials of LGD-4033 metabolites, the reference urine collected from volunteers who ingested 50 µg of LGD-4033 dissolved in 120 mL of drinkable yogurt was utilized in the experiment [14 (link)].
LC/MS-grade of methanol and acetonitrile were purchased from MerckMillipore (Darmstadt, Germany) and Fisher Chemical (Hampton, NH, USA), respectively. Disodium phosphate and monosodium phosphate ≥ 99% purity were supplied by Honeywell (Charlotte, NC, USA), while potassium carbonate and potassium bicarbonate, both HPLC grade, were purchased at POCH S.A. (Gliwice, Poland). In turn, β-glucuronidase (Escherichia coli) and HPLC-grade methyl tert-butyl ether were supplied by Roche (Basel, Switzerland) and J.T.Baker (Philipsburg, NJ, USA), respectively. The Millipore DirectQ UV3 system (Darmstadt, Germany) was used as the source of water (R > 18 MΩ/cm). Sample preparation of dietary supplement samples followed the protocol described in [19 (link),20 (link)].

Kwiatkowska D., Wicka M., Grucza K., Konarski P., Drapała A, & Kaliszewski P. (2023). Variation of Sequential Ligandrol (LGD-4033) Metabolite Levels in Routine Anti-Doping Urine Samples Detected with or without Other Xenobiotics. Molecules, 28(18), 6486.

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Quantitative Analysis of Ginsenosides

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Twenty-six reference standards (purity ≥ 98.0%) of ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2(R), Rg2(S), Rg3(R), Rg3(S), Rg5, Rg6, Rh1(R), Rh1(S), Rh2(R), Rh2(S), F1, F2, F3, F4, K, Mc, XVII, Y, and PPT(S)) were purchased from AMBO (Ambo institute, Seoul, Korea). HPLC grade methanol was purchased from Merck (Darmstadt, Germany) while LC-MS grade of methanol and formic acid were obtained from Merck (Darmstadt, Germany) and Sigma-Aldrich (St. Louis, Mo, USA), respectively.

Lee J., Han H., Yuan X., Park E., Lee J., & Kim J. (2021). A rapid, simultaneous and quantitative analysis of 26 ginsenosides in white and red Panax ginseng using LC–MS/MS. Applied Biological Chemistry, 64(1).

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LC/MS Analytical Reagents Protocol

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The solvents acetonitrile and methanol of LC/MS grade as well as ammonium formate and formic acid (mobile phase additives) were purchased from Sigma Aldrich, St. Louis, MO, USA and Merck, Darmstadt, Germany. Ultrapure Water (18.2 MΩ cm) was obtained from a Milli-Q purification system (Merck, Darmstadt, Germany). Saline solution (NaCl 0.9%) was purchased from VIOSER S.A.

Deda O., Virgiliou C., Orfanidis A, & Gika H.G. (2019). Study of Fecal and Urinary Metabolite Perturbations Induced by Chronic Ethanol Treatment in Mice by UHPLC-MS/MS Targeted Profiling. Metabolites, 9(10), 232.

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Quantification of NVP and Metabolites

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Standard, NVP was attained from TargetMol (Shanghai, China). Furthermore, 2-OH NVP and 3-OH NVP were supplied by Toronto Research Chemicals (Toronto, ON, Canada). NVP-d3 as internal standard (IS) of NVP and 2-OH NVP-d3 as IS of 2-OH NVP and 3-OH NVP were supplied by Toronto Research Chemicals (Toronto, ON, Canada). Ammonium acetate of LC-MS grade was obtained from Tedia (Fairfield, OH, USA), methanol of LC-MS grade was obtained from Sigma Aldrich (St. Louis, MO, USA). Deionized water was purchased from A.S. Watson Group (Hong Kong, China).
NVP, 2-OH NVP and 3-OH NVP were separately dissolved in methanol as stock solutions at concentrations of 1 mg/mL, 0.5 mg/mL and 50 μg/mL, respectively. The mixed working solution of the three compounds was needed to dilute the stock solution to the expected concentrations with methanol, such as 0.8–10,000 ng/mL for NVP and 0.4–250 ng/mL for 2-OH NVP and 3-OH NVP. The IS working solutions with high concentration were diluted with methanol to 50 ng/mL of NVP-d3 and 200 ng/mL of 2-OH NVP-d3. All of the above stock solutions were stored in the refrigerator.

Yang H., Chu L., Wu Y., Wang W., Yang J., Zhang Q., Qiao S., Li X., Shen Z., Zhou Y., Liu S, & Deng H. (2020). LC-MS/MS Quantification of Nevirapine and Its Metabolites in Hair for Assessing Long-Term Adherence. Molecules, 25(23), 5692.

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Carotenoid Quantification in Plasma

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The analyzed samples were −80°C stored K3-EDTA fasting plasma that had been drawn at baseline. Handling of all samples and standards was always done under cool conditions and avoiding exposure to light. Carotenoid standards: astaxanthin, canthaxanthin, E-β-apo-8′-carotenal, α-carotene, β-carotene, fucoxanthin, and lycopene were obtained from Sigma-Aldrich (St. Louis, MO, USA). Lutein was purchased from Cayman Chemical (Ann Arbor, MI, USA), zeaxanthin and β-cryptoxanthin were provided by Extrasynthese (Genay, Lyon, France). 13-Z-β-carotene and 9-Z-β-carotene were purchased from Carbosynth (Newbury, Berkshire, UK).
Methanol of LC-MS grade, n-hexane, ethanol, and methyl tert-butyl ether (MTBE) of HPLC grade, blank human plasma, and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. Ammonium acetate (AMAC) and acetic acid of HPLC grade were purchased from Panreac Quimica SLU (Barcelona, Spain). Ultrapure water (Milli-Q) was generated by a Millipore system (Bedford, MA, USA).

Marhuenda-Muñoz M., Domínguez-López I., Langohr K., Tresserra-Rimbau A., Martínez González M.Á., Salas-Salvadó J., Corella D., Zomeño M.D., Martínez J.A., Alonso-Gómez A.M., Wärnberg J., Vioque J., Romaguera D., López-Miranda J., Estruch R., Tinahones F.J., Lapetra J., Serra-Majem L., Bueno-Cavanillas A., Tur J.A., Martín-Sánchez V., Pintó X., Delgado-Rodríguez M., Matía-Martín P., Vidal J., Vázquez C., Daimiel L., Ros E., Toledo E., Fernández de la Puente Cervera M., Barragán R., Fitó M., Tojal-Sierra L., Gómez-Gracia E., Zazo J.M., Morey M., García-Ríos A., Casas R., Gómez-Pérez A.M., Santos-Lozano J.M., Vázquez-Ruiz Z., Atzeni A., Asensio E.M., Gili-Riu M.M., Bullon V., Moreno-Rodriguez A., Lecea O., Babio N., Peñas Lopez F., Gómez Melis G, & Lamuela-Raventós R.M. (2022). Circulating carotenoids are associated with favorable lipid and fatty acid profiles in an older population at high cardiovascular risk. Frontiers in Nutrition, 9, 967967.

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Carotenoid Analysis in Fasting Plasma

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K3-EDTA fasting plasma samples from the baseline blood extractions were analyzed. These samples were drawn in the first visit of the study, just after being randomly assigned to an intervention group and stored at −80 °C until use. All samples and standards were always handled avoiding exposure to light and under cool conditions. Carotenoid standards: astaxanthin, canthaxanthin, E-β-apo-8′-carotenal, α-carotene, β-carotene, fucoxanthin, and lycopene were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lutein was provided by Cayman Chemical (Ann Arbor, MI, USA), zeaxanthin and β-cryptoxanthin were purchased from Extrasynthese (Genay, Lyon, France). 13-Z-β-carotene and 9-Z-β-carotene were purchased from Carbosynth (Newbury, Berkshire, UK). Standards were stored in powder form at −20 °C and protected from light.
Methanol of LC-MS grade, n-hexane, ethanol and methyl tert-butyl ether (MTBE) of HPLC grade, blank human plasma and butylated hydroxytoluene (BHT) were obtained from Sigma-Aldrich. Ammonium acetate (AMAC) and acetic acid of HPLC grade were purchased from Panreac Quimica SLU (Barcelona, Spain). Ultrapure water (Milli-Q) was generated by a Millipore system (Bedford, MA, USA).

Marhuenda-Muñoz M., Rinaldi de Alvarenga J.F., Hernáez Á., Tresserra-Rimbau A., Martínez-González M.Á., Salas-Salvadó J., Corella D., Malcampo M., Martínez J.A., Alonso-Gómez Á.M., Wärnberg J., Vioque J., Romaguera D., López-Miranda J., Estruch R., Tinahones F.J., Lapetra J., Serra-Majem J.L., Bueno-Cavanillas A., Tur J.A., Sánchez V.M., Pintó X., Delgado-Rodríguez M., Matía-Martín P., Vidal J., Vázquez C., Daimiel L., Ros E., Serra-Mir M., Vázquez-Ruiz Z., Nishi S.K., Sorlí J.V., Zomeño M.D., Zulet M.A., Vaquero-Luna J., Carabaño-Moral R., Notario-Barandiaran L., Morey M., García-Ríos A., Gómez-Pérez A.M., Santos-Lozano J.M., Buil-Cosiales P., Basora J., Portolés O., Schröder H., Abete I., Salaverria-Lete I., Toledo E., Babio N., Fitó M., Martínez-Huélamo M, & Lamuela-Raventós R.M. (2021). High Fruit and Vegetable Consumption and Moderate Fat Intake Are Associated with Higher Carotenoid Concentration in Human Plasma. Antioxidants, 10(3), 473.

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Analytical-grade solvent preparation

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Pure water from a Milli-Q water purification system (Millipore, Bedford, MA, USA) was used. Acetonitrile and methanol of LC-MS grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Formic acid eluent additive for the mobile phase was purchased from Merck (Darmstadt, Germany).

, & Gumus Z.P. (2023). Assessment of Toxic Pyrrolizidine and Tropane Alkaloids in Herbal Teas and Culinary Herbs Using LC-Q-ToF/MS. Foods, 12(19), 3572.

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